Humoral Immune Response of Immunized Sows with Recombinant Proteins of Enterotoxigenic Escherichia coli

D. A. Pereira, C. Silva, M. Ono, O. Vidotto, M. C. Vidotto
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引用次数: 6

Abstract

Enteric disorders in pigs are related to the fimbriae F4 (K88), F5 (K99), F6 (987P), F41 and F18 of enterotoxigenic Escherichia coli (ETEC). Immunization of sows with adhesins is important to stimulate the production of antibodies and the consequent transfer of these to the piglets via colostrum to prevent diarrhea during the neonate period and after weaning. The objective of this study was to evaluate the immune response of the sows immunized with recombinant ETEC proteins (F4, F5, F6, F18 and F41). The immune response of the sows immunized with the recombinant proteins was compared with a commercial vaccine containing ETEC bacterins. The study was performed on a commercial farm and included nine pregnant sows divided into three groups: G1 was vaccinated with recombinant proteins (n = 3); G2 was vaccinated with the commercial vaccine (n = 3); and G3 was vaccinated with sterile buffered saline (PBS) (n = 3). All the sows were fed a balanced diet without antibiotics and water ad libitum. The recombinant fimbriae stimulated the specific humoral immune response of the immunized sows. There was a statistically significant increase in the levels of antibodies to the fimbriae F4 (K88), F5 (K99), F6 (987P) and F18 in the sows vaccinated with the recombinant proteins compared with the control group. The colostrum IgG titers for all fimbriae in all the immunized sows were significantly increased compared to the control group. Additionally, all the piglets exhibited significantly increased antibody levels relative to all fimbriae when compared with those in the unimmunized control group, demonstrating successful antibody transfer via colostrum of the sows to the piglets.
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产肠毒素大肠杆菌重组蛋白免疫母猪的体液免疫反应
猪肠道疾病与产肠毒素大肠杆菌(ETEC)菌毛F4 (K88)、F5 (K99)、F6 (987P)、F41和F18有关。用黏附素免疫母猪对于刺激抗体的产生和随后通过初乳将这些抗体转移到仔猪以预防新生儿期和断奶后的腹泻是重要的。本研究的目的是评价重组ETEC蛋白(F4, F5, F6, F18和F41)免疫母猪的免疫应答。用重组蛋白免疫母猪的免疫反应与含有ETEC细菌的商业疫苗进行了比较。该研究在一个商业农场进行,包括9头妊娠母猪,分为三组:G1接种重组蛋白(n = 3);G2接种市售疫苗(n = 3);G3接种无菌缓冲生理盐水(PBS) (n = 3)。所有母猪均饲喂不含抗生素的平衡饲粮和随意饮水。重组菌毛刺激了免疫母猪的特异性体液免疫反应。与对照组相比,接种重组蛋白的母猪毛膜F4 (K88)、F5 (K99)、F6 (987P)和F18抗体水平均有统计学意义的升高。与对照组相比,所有免疫组母猪初乳各菌膜IgG滴度均显著升高。此外,与未免疫对照组相比,所有仔猪相对于所有毛的抗体水平均显著增加,表明抗体通过母猪初乳成功转移到仔猪。
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