Comparative Efficacy of Different Inactivated Hydro-Pericardium Syndrome Vaccines Prepared from Infected Liver and Vero Cell Line Adapted Adeno Type 4 Virus

M. Mehmood, Huma Anwarul-Haq, F. Amin, S. Hussain, Ejaz Rafique, M. Ghani, M. Ismail, Fareeha Ghaffar
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Abstract

Hydro-Pericardium Syndrome (HPS) is viral problem of commercial poultry caused by aviadeno virus type-4. In Pakistan the problems have been controlled by administering inactivated infected liver homogenate vaccine (ILHV). The use of liver based HPS vaccines remained potential threat for having hypersensitivity reactions in poultry. The current study was carried out to compare the serological potency of HPS ILHV to vero cell line adopted vaccine in term of anti HPS-ELISA antibody titers. 14 HPS virus vaccines were prepared based on different concentration of antigen, type of adjuvants and source of virus substrate. Total of 160 birds were divided into 16 groups each containing 10 birds. At day of 14th age each bird of every group was injected with 0.3 ml dose of respective vaccine. It was observed that HPS infected liver based vaccine having 1 × 105.6, 1 × 105.6 and 1 × 103.6 bird lethal dose 50 induced 1092.10, 875.25 and 702.2 anti-HPS ELISA antibody titer respectively. The 20, 25 and 30 doses/gm HPS infected liver vaccine induced 110.4, 1071.9 and 1037.8 anti-HPS ELISA antibody titer respectively. Montanide based tissue culture HPS vaccine showed significantly higher 1148.45 anti-HPS ELISA antibody titer to aluminium hydroxide based vaccine (137.2) (P 5.6 TCID50 is serological potent against field infection. The vaccines based on such formulation could be prepared in future for effective immuno-prophylaxis against HPS virus.
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感染肝脏与Vero细胞系适应腺病毒4型病毒制备的不同心包水综合征灭活疫苗的疗效比较
心包积水综合征(HPS)是由4型禽腺病毒引起的商品家禽病毒性疾病。在巴基斯坦,这些问题已通过注射灭活的受感染肝匀浆疫苗得到控制。使用肝基HPS疫苗仍有可能对家禽产生过敏反应。本研究从抗HPS- elisa抗体滴度的角度比较了HPS- ILHV与vero细胞系疫苗的血清学效力。根据不同抗原浓度、佐剂种类和病毒底物来源制备了14种HPS病毒疫苗。160只鸟被分成16组,每组10只。14日龄时,每组每只鸡分别注射0.3 ml剂量的疫苗。结果表明,HPS感染肝基疫苗具有1 × 105.6、1 × 105.6和1 × 103.6鸟致死量50,抗HPS ELISA抗体效价分别为1092.10、875.25和702.2。20、25和30剂量/gm HPS感染肝疫苗的抗HPS ELISA抗体效价分别为110.4、1071.9和1037.8。基于Montanide的组织培养HPS疫苗的抗HPS ELISA抗体效价(1148.45)显著高于基于氧化铝的疫苗(137.2)(p5.6) (TCID50具有抗野外感染的血清学效力)。基于该配方的疫苗可用于未来对HPS病毒进行有效的免疫预防。
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