Towards portable flow cytometry: study on the use of air-sheath-based volume-efficient two-phase microfluidic systems

D. Huh, A. Tkaczyk, Hsien-Hung Wei, J. Grotberg, S. Takayama
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引用次数: 4

Abstract

Reports experimental studies on air-liquid two-phase stratified flows in polymeric microfluidic channels for volume-efficient and cost-effective flow cytometry. Different flow behaviors of liquid core streams sheathed by air flows were investigated by varying surface chemistry and geometry of poly(dimethylsiloxane) (PDMS) channels. Fluorescent beads and myoblast cells carried by stable liquid flows were analyzed by a digital photo-multiplier tube (PMT)-based optical detection system to analyze the number of particles in a sample.
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迈向便携式流式细胞术:基于空气护套的体积高效两相微流体系统的研究
报道了用于高效体积和低成本流式细胞术的聚合微流体通道中气液两相分层流动的实验研究。通过改变聚二甲基硅氧烷(PDMS)通道的表面化学和几何形状,研究了被空气包裹的液芯流的不同流动行为。采用基于数字光倍增管(PMT)的光学检测系统对稳定液体流动携带的荧光珠和成肌细胞进行分析,以分析样品中的颗粒数量。
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