Isolation and identification of steroidal saponins in Taiwanese Rhizoma Paridis cultivar (Paris formosana Hayata).

Abstract

台灣蚤休(Paris formosana Hayata)具有九個類固醇皂苷，其化學結構是利用液－液萃取配合高效能液相層析儀進行分離純化，再經液相層析質譜儀與核磁共振儀進行結構鑑定得知。此九個類固醇皂苷包括三個furostanol glycosides: 26-O-β-D-glucopyranosyl-22α-methoxyl-(25R)-furost-5-en-3β, 26-diol 3-O-α-L-rhamnopyranosyl-(1→4)-O-α-L-rhamnopyranosyl-(1→4)-O-[α-L-rhamnopyranosyl-(1→2)]-β-D-glucopyranoside, 26-O-β-D-glucopyranosyl-22α-methoxyl-(25R)-furost-5-en-3β, 26-diol 3-O-α-L-rhamnopyranosyl-(1→2)-O-[α-L-arabinofuranosyl-(1→4)]-β-Dglucopyranoside和26-O-β-D-glucopyranosyl-22α-methoxyl-(25R)-furost-5-en-3β, 26-diol 3-O-α-L-rhamnopyranosy-(1→2)-O-[β-D-glucopyranosyl (1→3)]-β-D-glucopyranoside，二個pennogenyl glycosides (spirostanol glycosides): 25 (R)-spirost-5-en-3β, 17-diol 3-O-α-L-rhamnopyranosy-(1→4)-O-α-L-rhamnopyranosyl-(1→4)-O-[α-L-rhamnopyranosyl-(1→2)]-β-D-glucopyranoside和(25R)-spirost-5-en-3β, 17-diol 3-O-α-L-rhamnopyranosyl-(1→2)-[α-L-arabinofuranosyl-(1→4)]-β-D-glucopyranoside以及四個diosgenyl glycosides (spirostanol glycosides): (25R)-spirost-5-en-3β-ol 3-O-α-L-rhamnopyranosyl-(1→4)-O-α-L-rhamnopyranosyl-(1→4)-O-[α-L-rhamnopyranosyl-(1→2)]-β-D-glucopyranoside, 3-O-α-L-rhamnopyranosyl-(1→2)-[β-D-glucopyranoside-(1→3)]-β-D-glucopyranoside, (25R)-spirost-5-en-3β-ol 3-O-α-L-rhamnopyranosyl-(1→2)-[α-L-arabinofuranosyl-(1→4)]-β-D-glucopyranoside (25R)-spirost-5-en-3β-ol和(25R)-spirost-5-en-3β-ol 3-O-α-Lrhamnopyranosyl-(1→2)-O-β-D-glucopyranoside.