Detection of class 1 integron-associated gene cassettes of multi-drug resistant Salmonella strains isolated from food at conventional markets in Ho Chi Minh city
Anh Vu Truong Huynh, Khue Tu Nguyen Hoang, Ha Huynh Yen, Hai Chu Van
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引用次数: 0
Abstract
After Salmonella strains were isolated from food samples collected randomly at conventional markets in several districts in Ho Chi Minh city, we evaluated antibiotic resistance by Kirby-Bauer methods, and the serotype name was assigned according to ISO/TR 6579-3:2014 and class 1 integron was investigated by PCR technique. As a result, there were seven distinguished serovars, including S. Kentucky (8 strains); S. Infantis (4 strains); S. Agona and S. Potsdam (2 strains); S. Saintpaul, S. Braenderup, S. Indiana (01 strain); OMF:1,z6:UT and 7:1,z6:UT (01 strain). The rate of multidrug-resistance Salmonella serovars carrying class 1 integron was 100% (21/21). The gene cassette region of class 1 integron accounted for 85.71% (18/21). The presence of mobile genetic factors in Salmonella in the study suggests that the bacteria can transmit or receive antibiotic resistance genes from other bacterial species in the natural environment. In addition, the research results provide scientific evidence for management decisions and raise awareness of effective antibiotic use in Ho Chi Minh City, Vietnam.
从胡志明市多个地区的传统市场随机采集的食品中分离沙门氏菌，采用Kirby-Bauer法进行耐药性鉴定，并根据ISO/TR 6579-3:2014进行血清型命名，采用PCR技术检测1类整合子。结果，共有7种不同的血清型，包括肯塔基沙门氏菌(8株);婴儿链球菌(4株);S. Agona和S. Potsdam(2株);S. saint - paul, S. Braenderup, S. Indiana(01菌株);OMF:1,z6:UT和7:1,z6:UT(01菌株)。携带1类整合子的多药耐药血清型沙门氏菌检出率为100%(21/21)。1类整合子基因盒区占85.71%(18/21)。研究中沙门氏菌中移动遗传因子的存在表明，该细菌可以在自然环境中传播或接受来自其他细菌物种的抗生素抗性基因。此外，研究结果为管理决策提供了科学依据，并提高了越南胡志明市有效使用抗生素的认识。
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