Pub Date : 2024-03-21DOI: 10.47866/2615-9252/vjfc.4209
Hanh Ninh Thi, Hoa Le Vinh, Ba Tran Hong, Quan Pham Van, Long Le Thanh, Loan Pham Thi
Botulinum neurotoxin (BoNT) is produced by the bacterium Clostridium botulinum and some other bacterial strains such as C. butyricum and C. baratii. It is considered the strongest toxin known to humans and can cause botulism. Botulinum neurotoxin can be fatal in humans and most botulism cases are caused by type A and B toxins. In this study, the research team used C. botulinum strains isolated from botulism poisoning cases in Vietnam, which were identified to produce type A and B toxins, from which we successfully transformed plasmids carrying genes specific for BoNT type A and type B genes based on the reference gene sequence according to TCVN 11395:2016. These plasmids were successfully used as template DNA for PCR reactions. In the PCR reaction, plasmids used to transform genes producing botulinum toxin types A and B have a detection limit of 102 copies/µL. The study has fully verified the validation parameters, with 100% accuracy and specificity.
肉毒杆菌神经毒素（BoNT）由肉毒梭菌和其他一些细菌菌株（如丁酸杆菌和巴拉蒂肉毒杆菌）产生。它被认为是人类已知的最强毒素，可导致肉毒中毒。肉毒杆菌神经毒素可致人死亡，大多数肉毒中毒病例都是由 A 型和 B 型毒素引起的。在这项研究中，研究小组使用了从越南肉毒中毒病例中分离出的肉毒杆菌菌株，经鉴定，这些菌株可产生A型和B型毒素，根据TCVN 11395:2016的参考基因序列，我们成功地从这些菌株中转化了携带BoNT A型和B型特异性基因的质粒。这些质粒被成功用作 PCR 反应的模板 DNA。在 PCR 反应中，用于转化产生 A 型和 B 型肉毒毒素基因的质粒的检测限为 102 个拷贝/微升。该研究完全验证了验证参数，准确率和特异性均为 100%。
{"title":"Producing positive control materials for template in PCR testing to detect botulinum neurotoxin types A and B genes","authors":"Hanh Ninh Thi, Hoa Le Vinh, Ba Tran Hong, Quan Pham Van, Long Le Thanh, Loan Pham Thi","doi":"10.47866/2615-9252/vjfc.4209","DOIUrl":"https://doi.org/10.47866/2615-9252/vjfc.4209","url":null,"abstract":"Botulinum neurotoxin (BoNT) is produced by the bacterium Clostridium botulinum and some other bacterial strains such as C. butyricum and C. baratii. It is considered the strongest toxin known to humans and can cause botulism. Botulinum neurotoxin can be fatal in humans and most botulism cases are caused by type A and B toxins. In this study, the research team used C. botulinum strains isolated from botulism poisoning cases in Vietnam, which were identified to produce type A and B toxins, from which we successfully transformed plasmids carrying genes specific for BoNT type A and type B genes based on the reference gene sequence according to TCVN 11395:2016. These plasmids were successfully used as template DNA for PCR reactions. In the PCR reaction, plasmids used to transform genes producing botulinum toxin types A and B have a detection limit of 102 copies/µL. The study has fully verified the validation parameters, with 100% accuracy and specificity.","PeriodicalId":12896,"journal":{"name":"Heavy metals and arsenic concentrations in water, agricultural soil, and rice in Ngan Son district, Bac Kan province, Vietnam","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140387931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-23DOI: 10.47866/2615-9252/vjfc.4211
Hao Le Thi Hong, Anh Nguyen Le Hoang, Huyen Nguyen Thi Thanh, Giang Nguyen Thi, Trong Pham Nhu, T. Nguyen Thanh, Trung Pham Quang, Thu Nguyen Thi Minh, Chien Dinh Viet, Nguyet Nguyen Anh, Dung Tran Viet, Trang Vu Thi
A cross-sectional descriptive study design aims to investigate food safety knowledge and practices of 422 vegetarian-food processors at 126 vegetarian food production, processing and trading establishments in Hanoi City. The results showed that 24,5% food processors had an acceptable general knowledge about food safety. Their knowledge about infrastructure and hygiene, and equipment and tools is 39.2% and 31.4% respectively. There are 32.1% of studied subjects reach acceptable knowledge about hygiene practices. The proportion of vegetarian food processors with evidence of being trained knowledge in food safety is only 31.1%. Research results are expected as the basis information to further solutions for intervention and management to ensure hygiene and safety for vegetarian foods.
{"title":"Food safety knowledge and practices of vegetarian food processors in Hanoi in 2022-2023","authors":"Hao Le Thi Hong, Anh Nguyen Le Hoang, Huyen Nguyen Thi Thanh, Giang Nguyen Thi, Trong Pham Nhu, T. Nguyen Thanh, Trung Pham Quang, Thu Nguyen Thi Minh, Chien Dinh Viet, Nguyet Nguyen Anh, Dung Tran Viet, Trang Vu Thi","doi":"10.47866/2615-9252/vjfc.4211","DOIUrl":"https://doi.org/10.47866/2615-9252/vjfc.4211","url":null,"abstract":"A cross-sectional descriptive study design aims to investigate food safety knowledge and practices of 422 vegetarian-food processors at 126 vegetarian food production, processing and trading establishments in Hanoi City. The results showed that 24,5% food processors had an acceptable general knowledge about food safety. Their knowledge about infrastructure and hygiene, and equipment and tools is 39.2% and 31.4% respectively. There are 32.1% of studied subjects reach acceptable knowledge about hygiene practices. The proportion of vegetarian food processors with evidence of being trained knowledge in food safety is only 31.1%. Research results are expected as the basis information to further solutions for intervention and management to ensure hygiene and safety for vegetarian foods.","PeriodicalId":12896,"journal":{"name":"Heavy metals and arsenic concentrations in water, agricultural soil, and rice in Ngan Son district, Bac Kan province, Vietnam","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140435533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-02DOI: 10.47866/2615-9252/vjfc.4204
Bach Ta Ngoc, Viet Nguyen Xuan, Anh Nguyen Van, Trung Pham Quang, Thu Nguyen Thi Minh, Thu Phung Thi, Ky Vu Hong, Manh Do Hung, Lam Vu Dinh, Dan Nguyen Huy, Anh Trinh Xuan, Le Ngo Thi Hong
In this study, we developed a non-enzymatic electrochemical sensor using a porous graphene electrode modified with ZnO nanoparticles (ZnO/fPGE sensor) to determine xanthine (XA) content. The ZnO/fPGE sensor is fabricated using a hydrothermal method and CO2 infrared laser writing technique on a polyimide film. The morphology, structure, and properties of the ZnO/fPGE were meticulously characterized using Raman spectroscopy, field-emission scanning electron microscopy (FE-SEM), and Von-Ampe spectroscopy methods. The ZnO/fPGE sensor exhibited a broad linear response range from 1 µM to 100 µM, a low limit of detection (LOD) of 0.29 µM, high sensitivity at 7.05 µA.µM⁻¹.cm⁻², and demonstrated effective resistance to common interferences such as uric acid, ascorbic acid, dopamine, glucose, and xanthine. Notably, the ZnO/fPGE sensor has created a conducive electrical environment for the advancement of high-performance electrochemical biosensors, specifically for the precise determination of xanthine levels in meat and fish products.
{"title":"Research and development of non-enzymatic electrochemical sensors utilizing porous ZnO/graphene electrodes to determine xanthine","authors":"Bach Ta Ngoc, Viet Nguyen Xuan, Anh Nguyen Van, Trung Pham Quang, Thu Nguyen Thi Minh, Thu Phung Thi, Ky Vu Hong, Manh Do Hung, Lam Vu Dinh, Dan Nguyen Huy, Anh Trinh Xuan, Le Ngo Thi Hong","doi":"10.47866/2615-9252/vjfc.4204","DOIUrl":"https://doi.org/10.47866/2615-9252/vjfc.4204","url":null,"abstract":"In this study, we developed a non-enzymatic electrochemical sensor using a porous graphene electrode modified with ZnO nanoparticles (ZnO/fPGE sensor) to determine xanthine (XA) content. The ZnO/fPGE sensor is fabricated using a hydrothermal method and CO2 infrared laser writing technique on a polyimide film. The morphology, structure, and properties of the ZnO/fPGE were meticulously characterized using Raman spectroscopy, field-emission scanning electron microscopy (FE-SEM), and Von-Ampe spectroscopy methods. The ZnO/fPGE sensor exhibited a broad linear response range from 1 µM to 100 µM, a low limit of detection (LOD) of 0.29 µM, high sensitivity at 7.05 µA.µM⁻¹.cm⁻², and demonstrated effective resistance to common interferences such as uric acid, ascorbic acid, dopamine, glucose, and xanthine. Notably, the ZnO/fPGE sensor has created a conducive electrical environment for the advancement of high-performance electrochemical biosensors, specifically for the precise determination of xanthine levels in meat and fish products.","PeriodicalId":12896,"journal":{"name":"Heavy metals and arsenic concentrations in water, agricultural soil, and rice in Ngan Son district, Bac Kan province, Vietnam","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140462433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-24DOI: 10.47866/2615-9252/vjfc.4206
Ngoc Nguyen Nhu, Nghia Nguyen Mau
The aim of this study was to identify the biodegradation abilities of some indigenous microorganisms applied in coffee husk treatment into organic substrates. There were three useful strains in coffee husks that were isolated and identified as Bacillus thuringiensis CF2, Bacillus subtilis CF4, and 01 fungal strain Cladosporium tenuissimum CF17. The biodegradation diversity of organic compounds in coffee husks as well as nitrogen fixation and 3- Indol acetic acid (IAA) production of those strains were tested. The biomass of three strains also were collected from fermentation broth and mixed into biomass solution with 1:1:1 (v/v/v) proportion in volume and then were incubated with dry coffee husks. The results showed that the most effective incubation formula is mixing 500 mL of the biomass solution mixture with 20 kg of dry coffee husks for 8 weeks. The quality of the substrates from coffee husk waste after 08 weeks of incubation is shown through a number of indicators meeting the quality of organic substrates specified in TCVN 7185: 2002 and testing the quality of the substrates. The organic substrates from coffee husks were used to grow sprouts and accessed. The weight of sprouts obtained when sowing on coffee husk substrates reached 69.7 grams while it reached 43.2 grams on a commercial substrate in the same area of 30 cm2.
{"title":"Study on coffee husk treatment by microorganisms","authors":"Ngoc Nguyen Nhu, Nghia Nguyen Mau","doi":"10.47866/2615-9252/vjfc.4206","DOIUrl":"https://doi.org/10.47866/2615-9252/vjfc.4206","url":null,"abstract":"The aim of this study was to identify the biodegradation abilities of some indigenous microorganisms applied in coffee husk treatment into organic substrates. There were three useful strains in coffee husks that were isolated and identified as Bacillus thuringiensis CF2, Bacillus subtilis CF4, and 01 fungal strain Cladosporium tenuissimum CF17. The biodegradation diversity of organic compounds in coffee husks as well as nitrogen fixation and 3- Indol acetic acid (IAA) production of those strains were tested. The biomass of three strains also were collected from fermentation broth and mixed into biomass solution with 1:1:1 (v/v/v) proportion in volume and then were incubated with dry coffee husks. The results showed that the most effective incubation formula is mixing 500 mL of the biomass solution mixture with 20 kg of dry coffee husks for 8 weeks. The quality of the substrates from coffee husk waste after 08 weeks of incubation is shown through a number of indicators meeting the quality of organic substrates specified in TCVN 7185: 2002 and testing the quality of the substrates. The organic substrates from coffee husks were used to grow sprouts and accessed. The weight of sprouts obtained when sowing on coffee husk substrates reached 69.7 grams while it reached 43.2 grams on a commercial substrate in the same area of 30 cm2.","PeriodicalId":12896,"journal":{"name":"Heavy metals and arsenic concentrations in water, agricultural soil, and rice in Ngan Son district, Bac Kan province, Vietnam","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140496681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-16DOI: 10.47866/2615-9252/vjfc.4203
Trang Nguyen Linh, Trang Tran Thi Thu, Anh Dinh Hoang, Nguyen Nguyen Thi Thao, Hong Ha Tran Thi, Hang Do Thi Thu, Phuong Kieu Thi Lan, Cuong Dang Huu, Ket Dang Van, Huyen Nguyen Thi, Thao Le Thi Phuong, Hao Le Thi Hong, Huong Nguyen Thi Anh, Hoa Nguyen Thi Minh
Controlling the level of crop protection chemical residues in food is always a matter of concern. Certified reference materials (CRM) or reference materials (RM) are used in crop protection chemical residue analysis to validate analytical methods and evaluate the quality of measurement results. Imidacloprid is a common crop protection chemical used in tomato crops. A comprehensive study of the production, storage, transport, and utilization of CRM imidacloprid in tomato matrix was conducted. After comparing the two frozen and freeze-dried production processes, the freeze-dried production process was concluded to be superior. The CRM characterization process complies with ISO 17034:2016. The concentration of imidacloprid in tomato CRM is consistent with the maximum residue limit of imidacloprid according to the Codex and regulations of Circular 50/2016/TT-BYT of the Ministry of Health of Vietnam. At 4°C storage temperatures, freeze-dried tomato CRM was predicted to be stable for at least 552 days, while at -20°C the shelf life was at least 704 days.
{"title":"A comprehensive study on reference material of Imidacloprid in tomato matrix","authors":"Trang Nguyen Linh, Trang Tran Thi Thu, Anh Dinh Hoang, Nguyen Nguyen Thi Thao, Hong Ha Tran Thi, Hang Do Thi Thu, Phuong Kieu Thi Lan, Cuong Dang Huu, Ket Dang Van, Huyen Nguyen Thi, Thao Le Thi Phuong, Hao Le Thi Hong, Huong Nguyen Thi Anh, Hoa Nguyen Thi Minh","doi":"10.47866/2615-9252/vjfc.4203","DOIUrl":"https://doi.org/10.47866/2615-9252/vjfc.4203","url":null,"abstract":"Controlling the level of crop protection chemical residues in food is always a matter of concern. Certified reference materials (CRM) or reference materials (RM) are used in crop protection chemical residue analysis to validate analytical methods and evaluate the quality of measurement results. Imidacloprid is a common crop protection chemical used in tomato crops. A comprehensive study of the production, storage, transport, and utilization of CRM imidacloprid in tomato matrix was conducted. After comparing the two frozen and freeze-dried production processes, the freeze-dried production process was concluded to be superior. The CRM characterization process complies with ISO 17034:2016. The concentration of imidacloprid in tomato CRM is consistent with the maximum residue limit of imidacloprid according to the Codex and regulations of Circular 50/2016/TT-BYT of the Ministry of Health of Vietnam. At 4°C storage temperatures, freeze-dried tomato CRM was predicted to be stable for at least 552 days, while at -20°C the shelf life was at least 704 days.","PeriodicalId":12896,"journal":{"name":"Heavy metals and arsenic concentrations in water, agricultural soil, and rice in Ngan Son district, Bac Kan province, Vietnam","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140505924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-10DOI: 10.47866/2615-9252/vjfc.4207
Bich Vo Thi Nhu, Vu Truong Huynh Anh
In this study, the ability to detect Bacillus cereus of the new chromogenic agar media RAPID’B.cereus (RBC) was compared with the standard selective culture media Mannitol Egg Yolk Polymyxin (MYP) recommended by food authorities for isolation, identification, and quantification of Bacillus cereus. Two types of media were used to conduct the assessment on five categories of food, including cereals and cereal products; flour and starch; vegetables and vegetable products; milk and dairy products; and confectionery (n = 164). The results of the survey Bacillus cereus contamination on RBC (75.61%), MYP (70.12%), of which 46.09% of the samples didn’t meet the standards of the Ministry of Health on the maximum allowable limit of Bacillus cereus in food. Conducting performance testing of RBC according to ISO 16140-2:2016 with parameters: growth, selectivity, specificity, relative trueness and accuracy profile, the analysis results all met the criteria according to the standard: the development (PR) of the two media were 0.97 and 0.81 respectively; a high level of background microflora was present on the MYP plates while if it was not the case on the RBC plates; both media allowed the detection of Bacillus cereus with a difference of 0.14. Compared with the acceptance criteria as prescribed, the categories used in the study were all suitable for assessment in the new media with the accuracy between the two methods being equivalent.
{"title":"Evaluation of the ability to detect Bacillus cereus in food of chromogenic agar media RAPID’B.cereus according to ISO 7932:2004","authors":"Bich Vo Thi Nhu, Vu Truong Huynh Anh","doi":"10.47866/2615-9252/vjfc.4207","DOIUrl":"https://doi.org/10.47866/2615-9252/vjfc.4207","url":null,"abstract":"In this study, the ability to detect Bacillus cereus of the new chromogenic agar media RAPID’B.cereus (RBC) was compared with the standard selective culture media Mannitol Egg Yolk Polymyxin (MYP) recommended by food authorities for isolation, identification, and quantification of Bacillus cereus. Two types of media were used to conduct the assessment on five categories of food, including cereals and cereal products; flour and starch; vegetables and vegetable products; milk and dairy products; and confectionery (n = 164). The results of the survey Bacillus cereus contamination on RBC (75.61%), MYP (70.12%), of which 46.09% of the samples didn’t meet the standards of the Ministry of Health on the maximum allowable limit of Bacillus cereus in food. Conducting performance testing of RBC according to ISO 16140-2:2016 with parameters: growth, selectivity, specificity, relative trueness and accuracy profile, the analysis results all met the criteria according to the standard: the development (PR) of the two media were 0.97 and 0.81 respectively; a high level of background microflora was present on the MYP plates while if it was not the case on the RBC plates; both media allowed the detection of Bacillus cereus with a difference of 0.14. Compared with the acceptance criteria as prescribed, the categories used in the study were all suitable for assessment in the new media with the accuracy between the two methods being equivalent.","PeriodicalId":12896,"journal":{"name":"Heavy metals and arsenic concentrations in water, agricultural soil, and rice in Ngan Son district, Bac Kan province, Vietnam","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140511067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-08DOI: 10.47866/2615-9252/vjfc.4208
Thuan Nguyen Vu, Chien Vien Chinh, Trieu Tuong Quoc, Anh Hoang Khac Tuan, Hiền Nguyễn Thị Thu, H. Do Thi Thu
The cross-sectional descriptive study was conducted at 65 bottled drink water product production facilities in Dak Lak province in 2023. The objectives were to determine the prevalence of bacterial contamination in bottled drinking water products at some production facilities in Dak Lak province and analyze related factors. The results indicated that 42/65 (64,0%) of samples did not meet the bacterial requirements according to QCVN 6-1:2010/BYT. Unsatisfactory bacterial criteria included Coliform (50.5%), P. aeruginosa (50.5%), and Clostridia (4.6%). All samples were not detected for E. coli and Streptococci feacal. The main factors influencing bacterial contamination of bottled drinking water were the layout design of the production area; factory structure, means of washing and disinfecting hands, sanitary conditions of the jar, practice of personal hygiene, practice cleaning the jar, and practice of packaging the products.
{"title":"The assessment of bacterial contamination in bottled drinking water products in Dak Lak province in 2023 and its related factors","authors":"Thuan Nguyen Vu, Chien Vien Chinh, Trieu Tuong Quoc, Anh Hoang Khac Tuan, Hiền Nguyễn Thị Thu, H. Do Thi Thu","doi":"10.47866/2615-9252/vjfc.4208","DOIUrl":"https://doi.org/10.47866/2615-9252/vjfc.4208","url":null,"abstract":"The cross-sectional descriptive study was conducted at 65 bottled drink water product production facilities in Dak Lak province in 2023. The objectives were to determine the prevalence of bacterial contamination in bottled drinking water products at some production facilities in Dak Lak province and analyze related factors. The results indicated that 42/65 (64,0%) of samples did not meet the bacterial requirements according to QCVN 6-1:2010/BYT. Unsatisfactory bacterial criteria included Coliform (50.5%), P. aeruginosa (50.5%), and Clostridia (4.6%). All samples were not detected for E. coli and Streptococci feacal. The main factors influencing bacterial contamination of bottled drinking water were the layout design of the production area; factory structure, means of washing and disinfecting hands, sanitary conditions of the jar, practice of personal hygiene, practice cleaning the jar, and practice of packaging the products.","PeriodicalId":12896,"journal":{"name":"Heavy metals and arsenic concentrations in water, agricultural soil, and rice in Ngan Son district, Bac Kan province, Vietnam","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140512447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-05DOI: 10.47866/2615-9252/vjfc.4118
Huyen Trang Luu Thi
Nutritional supplements providing bioavailable essential minerals, such as zinc (Zn) and magnesium (Mg), in forms of absorbable salts (e.g., gluconate, stearate, lactate, etc.) have been widely used. However, it should be noted that alongside these supplements, some excipients, including inorganic salts like zinc sulfate and magnesium sulfate, are frequently employed in pharmaceutical and nutraceutical products. Conventional analytical methods for total metal contents could not distinguish actual concentrations of bioavailable forms, suggesting the need for simultaneous analysis of both these essential minerals and their corresponding salts. In this study, we applied the capillary electrophoresis with capacitively coupled contactless conductivity detection (CE-C4D) method to simultaneously measure zinc and gluconate in nutritional supplements. The optimal analytical parameters comprise: (1) 10 mM Tris/Ace electrolyte solution at pH 5.0; (2) silica capillary with an internal diameter of 50 μm and an effective length of 30 cm; (3) separation voltage of +15 kV; (4) hydrodynamic injection mode with siphoning technique at 10 cm height and injection time of 20 s. With these conditions, limit of detections of 1.0 mg/L for zinc and 1.5 mg/L for gluconate were achieved. The method has good repeatability (RSD < 3%) and good recovery (89-103% for zinc and 88-107% for gluconate). The validated method was applied to determine zinc and gluconate concentrations in three commercially available nutritional supplement samples. To ascertain the validity of the results, cross-validation was performed using the high-performance liquid chromatography with photodiode array detection (HPLCPDA) for gluconate and the inductively coupled plasma optical emission spectrometry (ICPOES) for zinc, thereby reinforcing the reliability of the CE-C4D method.
{"title":"Study on simultaneous determination of zinc and gluconate in nutritional supplements by CE-C4D","authors":"Huyen Trang Luu Thi","doi":"10.47866/2615-9252/vjfc.4118","DOIUrl":"https://doi.org/10.47866/2615-9252/vjfc.4118","url":null,"abstract":"Nutritional supplements providing bioavailable essential minerals, such as zinc (Zn) and magnesium (Mg), in forms of absorbable salts (e.g., gluconate, stearate, lactate, etc.) have been widely used. However, it should be noted that alongside these supplements, some excipients, including inorganic salts like zinc sulfate and magnesium sulfate, are frequently employed in pharmaceutical and nutraceutical products. Conventional analytical methods for total metal contents could not distinguish actual concentrations of bioavailable forms, suggesting the need for simultaneous analysis of both these essential minerals and their corresponding salts. In this study, we applied the capillary electrophoresis with capacitively coupled contactless conductivity detection (CE-C4D) method to simultaneously measure zinc and gluconate in nutritional supplements. The optimal analytical parameters comprise: (1) 10 mM Tris/Ace electrolyte solution at pH 5.0; (2) silica capillary with an internal diameter of 50 μm and an effective length of 30 cm; (3) separation voltage of +15 kV; (4) hydrodynamic injection mode with siphoning technique at 10 cm height and injection time of 20 s. With these conditions, limit of detections of 1.0 mg/L for zinc and 1.5 mg/L for gluconate were achieved. The method has good repeatability (RSD < 3%) and good recovery (89-103% for zinc and 88-107% for gluconate). The validated method was applied to determine zinc and gluconate concentrations in three commercially available nutritional supplement samples. To ascertain the validity of the results, cross-validation was performed using the high-performance liquid chromatography with photodiode array detection (HPLCPDA) for gluconate and the inductively coupled plasma optical emission spectrometry (ICPOES) for zinc, thereby reinforcing the reliability of the CE-C4D method.","PeriodicalId":12896,"journal":{"name":"Heavy metals and arsenic concentrations in water, agricultural soil, and rice in Ngan Son district, Bac Kan province, Vietnam","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139362587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-23DOI: 10.47866/2615-9252/vjfc.4079
Hung Le Thanh, Phuong Trieu Viet, Hoa Ngo Quynh, Hang Nguyen Thuy
At the end of 2022, Vietnam's standard system (TCVN) had 2026 national standards related to the food chain (food and feed), of which the number of test method standards accounts for nearly 70 %. The test method standards have played an extremely important role in the food chain. The harmonization of TCVN on test methods for the food chain according to international standards (ISO standards) is always a priority in the process of developing and perfecting the TCVN system. Moreover, there are also many TCVNs built on the basis of harmonizing (or referencing) methods of the International Association of Official Analytical Collaboration (AOAC International). This article analyzed a number of cases in which the AOAC method is referenced in the national standards and national technical regulations of Vietnam; the harmonization of national standards according to the AOAC method and the use of the AOAC method in a number of public and non-public laboratories. From the above analysis results, it is possible to comment on the trend of using AOAC methods in testing activities in Vietnam.
{"title":"The tendency of referencing to AOAC methods in TCVNs, QCVNs and harmonizing TCVNs in the food chain according to the AOAC methods","authors":"Hung Le Thanh, Phuong Trieu Viet, Hoa Ngo Quynh, Hang Nguyen Thuy","doi":"10.47866/2615-9252/vjfc.4079","DOIUrl":"https://doi.org/10.47866/2615-9252/vjfc.4079","url":null,"abstract":"At the end of 2022, Vietnam's standard system (TCVN) had 2026 national standards related to the food chain (food and feed), of which the number of test method standards accounts for nearly 70 %. The test method standards have played an extremely important role in the food chain. The harmonization of TCVN on test methods for the food chain according to international standards (ISO standards) is always a priority in the process of developing and perfecting the TCVN system. Moreover, there are also many TCVNs built on the basis of harmonizing (or referencing) methods of the International Association of Official Analytical Collaboration (AOAC International). This article analyzed a number of cases in which the AOAC method is referenced in the national standards and national technical regulations of Vietnam; the harmonization of national standards according to the AOAC method and the use of the AOAC method in a number of public and non-public laboratories. From the above analysis results, it is possible to comment on the trend of using AOAC methods in testing activities in Vietnam.","PeriodicalId":12896,"journal":{"name":"Heavy metals and arsenic concentrations in water, agricultural soil, and rice in Ngan Son district, Bac Kan province, Vietnam","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88133104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-21DOI: 10.47866/2615-9252/vjfc.4080
Tam Phan Thanh, Ánh Trương Nguyệt
Salted and smoked pork leg products (Ham) have become prevalent in the world for a long time. Although these foods have appeared in Vietnam for a few years, consumers warmly received them due to their tasty specificity. The quality of this product group is affected by several technological factors, including the composition of salty solution and additives, the technique of bringing meat, the heat treatment process (drying, smoking, steaming), and storage conditions. Therefore, the research of production technology to ensure food safety and hygiene are essential. Furthermore, level 2 orthogonal experimental matrix and optimization of technological factors have been applied for the best product quality. The study provides a perfect example of the production technology of bringing and drying meat products to ensure food hygiene and safety for consumers, contributing to promoting the domestic meat processing industry to develop in the direction of industrialization, safety, and integration.
{"title":"Research on production technology of smoked-dried salted pork leg meat to ensure food hygiene and safety","authors":"Tam Phan Thanh, Ánh Trương Nguyệt","doi":"10.47866/2615-9252/vjfc.4080","DOIUrl":"https://doi.org/10.47866/2615-9252/vjfc.4080","url":null,"abstract":"Salted and smoked pork leg products (Ham) have become prevalent in the world for a long time. Although these foods have appeared in Vietnam for a few years, consumers warmly received them due to their tasty specificity. The quality of this product group is affected by several technological factors, including the composition of salty solution and additives, the technique of bringing meat, the heat treatment process (drying, smoking, steaming), and storage conditions. Therefore, the research of production technology to ensure food safety and hygiene are essential. Furthermore, level 2 orthogonal experimental matrix and optimization of technological factors have been applied for the best product quality. The study provides a perfect example of the production technology of bringing and drying meat products to ensure food hygiene and safety for consumers, contributing to promoting the domestic meat processing industry to develop in the direction of industrialization, safety, and integration.","PeriodicalId":12896,"journal":{"name":"Heavy metals and arsenic concentrations in water, agricultural soil, and rice in Ngan Son district, Bac Kan province, Vietnam","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90162511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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