Evaluation effect of nanocarriers of AmB on pathogenics fungal

Abstract

nm. The cytotoxicity assay demonstrated that NanoAmB and nanocarriers without AmB does not show cytotoxicity against mammalian cells at concentrations ranging from 10 μg/mL to 0.1 μg/mL. However NanoAmB demonstrated that cytotoxic effect against C. albicans and C. neoformans after 24 hours in all concentration analyzed (10 μg/mL to 0.1 μg/mL) (p<0.05). However, nanocarriers without AmB showed a cytotoxic effect from 10 to 2.5 μg/Ml. NanoAmB showed an effect from 1.25 to 0.05 μg/mL in both fungal species and a partial effect at concentration up to 0.025 μg/mL only in C. albicans (p<0.05). Concentrations lower than 0.025 showed no cytotoxic effect. Conclusion: Nanocarriers acted as an enhancing agent, potentializing the inhibitory growth effects of AmB on pathogenic fungi. Other novel antifungal therapeutic strategies using NanoAmB, isolated or in combination with mAbs, should be considered in the future.