Subarachnoid hemorrhage (SAH) remains a potentially devastating cerebrovascular disease with a high morbidity and mortality rate, irrespective of treatment. The disease still has a 40-50% mortality rate with a 70% rate of cerebral vasospasm in those patients. The release of cytokines has been implicated in the development and progression of SAH. In this paper, we will explore the role of cytokines in aneurysmal subarachnoid hemorrhage (aSAH), including their effects on the inflammatory response, cerebral vasospasm, blood-brain barrier disruption, and neuronal damage. We also identify the role of the glymphatic system in progression of aSAH. The review will also briefly touch upon current research on potential therapeutic targets aimed at modulating cytokine activity in patients with aSAH. This review aims to give an in-depth review of the cytokines involved in aSAH and serve as a catalyst to research directed towards treatment options for aSAH.
{"title":"Cytokines and subarachnoid hemorrhage","authors":"FNU Ruchika, Siddharth Shah, Maliya Delawan, Neupane Durga, Brandon Lucke-Wold","doi":"10.59400/ivd.v3i1.55","DOIUrl":"https://doi.org/10.59400/ivd.v3i1.55","url":null,"abstract":"Subarachnoid hemorrhage (SAH) remains a potentially devastating cerebrovascular disease with a high morbidity and mortality rate, irrespective of treatment. The disease still has a 40-50% mortality rate with a 70% rate of cerebral vasospasm in those patients. The release of cytokines has been implicated in the development and progression of SAH. In this paper, we will explore the role of cytokines in aneurysmal subarachnoid hemorrhage (aSAH), including their effects on the inflammatory response, cerebral vasospasm, blood-brain barrier disruption, and neuronal damage. We also identify the role of the glymphatic system in progression of aSAH. The review will also briefly touch upon current research on potential therapeutic targets aimed at modulating cytokine activity in patients with aSAH. This review aims to give an in-depth review of the cytokines involved in aSAH and serve as a catalyst to research directed towards treatment options for aSAH.","PeriodicalId":8089,"journal":{"name":"Annals of the symposium: vaccines, biopharmaceuticals, in vitro diagnosis, management, other related themes","volume":"266 ","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135872411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Subarachnoid hemorrhage (SAH) remains a potentially devastating cerebrovascular disease with a high morbidity and mortality rate, irrespective of treatment. The disease still has a 40-50% mortality rate with a 70% rate of cerebral vasospasm in those patients. The release of cytokines has been implicated in the development and progression of SAH. In this paper, we will explore the role of cytokines in aneurysmal subarachnoid hemorrhage (aSAH), including their effects on the inflammatory response, cerebral vasospasm, blood-brain barrier disruption, and neuronal damage. We also identify the role of the glymphatic system in progression of aSAH. The review will also briefly touch upon current research on potential therapeutic targets aimed at modulating cytokine activity in patients with aSAH. This review aims to give an in-depth review of the cytokines involved in aSAH and serve as a catalyst to research directed towards treatment options for aSAH.
{"title":"Cytokines and subarachnoid hemorrhage","authors":"FNU Ruchika, Siddharth Shah, Maliya Delawan, Neupane Durga, Brandon Lucke-Wold","doi":"10.59400/ivd.v1i1.55","DOIUrl":"https://doi.org/10.59400/ivd.v1i1.55","url":null,"abstract":"Subarachnoid hemorrhage (SAH) remains a potentially devastating cerebrovascular disease with a high morbidity and mortality rate, irrespective of treatment. The disease still has a 40-50% mortality rate with a 70% rate of cerebral vasospasm in those patients. The release of cytokines has been implicated in the development and progression of SAH. In this paper, we will explore the role of cytokines in aneurysmal subarachnoid hemorrhage (aSAH), including their effects on the inflammatory response, cerebral vasospasm, blood-brain barrier disruption, and neuronal damage. We also identify the role of the glymphatic system in progression of aSAH. The review will also briefly touch upon current research on potential therapeutic targets aimed at modulating cytokine activity in patients with aSAH. This review aims to give an in-depth review of the cytokines involved in aSAH and serve as a catalyst to research directed towards treatment options for aSAH.","PeriodicalId":8089,"journal":{"name":"Annals of the symposium: vaccines, biopharmaceuticals, in vitro diagnosis, management, other related themes","volume":"22 8","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135809196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Renato Carvalho, I. Santos, Lucas Silva, A. Sobrinho, A. Bergamo
Objective: Development of an analytical UPLC method to identify each one of 15 amino acids in a mixture used for MMR vaccine production, for implementation in Bio-Manguinhos physicochemical quality control. Methodology: Amino acids mixture was derivatized with AQC (6-aminoquinolyl-N-hydroxysuccinimidyl carbamate) for detection by Photodiode Array (PDA). Analysis was performed by UPLC reverse-phase, in a gradient with mobile phases A as 140mM sodium acetate and 17 mM triethylamine (TEA) and B as 60% (volume) acetonitrile in water with column at 37 o C. The influence of pH were evaluated at range of 3,4 – 6,8 in mobile phase A and the identification of amino acids was made by comparison to reference standards. A full factorial design with 3 factors (pH, mass of sodium acetate and volume of TEA) and 2 levels with center point, was conducted to evaluate robustness. Selectivity was evaluated against other amino acids absent in the mixture. Results: All 15 amino acids were efficiently separated with a total run time of 19 min. The use of lower pH values allowed initial peaks with better symmetry and sharper. Isoleucine/Leucine were separated with Resolution > 1,5 in all experiments. Pareto’s graphic showed that pH was the only significant factor. From experimental data an optimization was made and was achieved a critical pair resolution of R > 1,6. The optimized conditions was: pH = 3,444; 140,7 mM sodium acetate; and 16,8 mM TEA. The method was selective in relation to Asparagine, Aspartic Acid, Glutamic Acid and Cysteine, and could be evaluated as internal standards for quantification. AQC derivatization products shown to be stable for more than 3 weeks for a qualitative analysis. Conclusion: The method developed shown to be ideal for quality control analysis considering its short derivatization step with a long product stability, speed and robustness for qualitative analysis, and the potential for quantify pure and mixed amino acid. This method is now ready to be validated and implemented at Bio-Manguinhos physicochemical quality control routine.
目的:建立一种高效液相色谱(UPLC)分析方法，用于MMR疫苗生产混合物中15种氨基酸的鉴定，用于生物曼金诺的理化质量控制。方法:用AQC(6-氨基喹啉- n -羟基琥珀酰氨基甲酸酯)衍生化氨基酸混合物，用光电二极管阵列(PDA)检测。采用UPLC反相分析，流动相a为140mM乙酸钠和17mm三乙胺(TEA)，流动相B为60%(体积)乙腈，柱温为37℃。流动相a在3、4 ~ 6、8范围内评价pH值的影响，并与参比标准品进行氨基酸鉴定。采用3个因素(pH、乙酸钠质量和TEA体积)和2个中心点水平的全因子设计来评价稳健性。对混合物中不存在的其他氨基酸进行选择性评价。结果:所有15种氨基酸均有效分离，总运行时间为19 min。使用较低的pH值可以使初始峰具有更好的对称性和更清晰。异亮氨酸/亮氨酸的分离分辨率均> 1、5。帕累托图显示pH值是唯一重要的因素。根据实验数据进行优化，获得了R > 1,6的临界对分辨率。优化条件为:pH = 3444;140、7 mM醋酸钠;16.8 mM TEA。该方法对天冬氨酸、天冬氨酸、谷氨酸和半胱氨酸具有选择性，可作为定量的内标。AQC衍生化产品在定性分析中稳定超过3周。结论:该方法衍生化步骤短，产品稳定性好，定性分析速度快，稳健性好，具有定量分析纯和混合氨基酸的潜力，是一种理想的质量控制分析方法。该方法现已准备好在Bio-Manguinhos理化质量控制程序中进行验证和实施。
{"title":"Development of amino acids identifying method by UPLC-PDA in mixture solution for MMR vaccine production","authors":"Renato Carvalho, I. Santos, Lucas Silva, A. Sobrinho, A. Bergamo","doi":"10.35259/isi.2022_52204","DOIUrl":"https://doi.org/10.35259/isi.2022_52204","url":null,"abstract":"Objective: Development of an analytical UPLC method to identify each one of 15 amino acids in a mixture used for MMR vaccine production, for implementation in Bio-Manguinhos physicochemical quality control. Methodology: Amino acids mixture was derivatized with AQC (6-aminoquinolyl-N-hydroxysuccinimidyl carbamate) for detection by Photodiode Array (PDA). Analysis was performed by UPLC reverse-phase, in a gradient with mobile phases A as 140mM sodium acetate and 17 mM triethylamine (TEA) and B as 60% (volume) acetonitrile in water with column at 37 o C. The influence of pH were evaluated at range of 3,4 – 6,8 in mobile phase A and the identification of amino acids was made by comparison to reference standards. A full factorial design with 3 factors (pH, mass of sodium acetate and volume of TEA) and 2 levels with center point, was conducted to evaluate robustness. Selectivity was evaluated against other amino acids absent in the mixture. Results: All 15 amino acids were efficiently separated with a total run time of 19 min. The use of lower pH values allowed initial peaks with better symmetry and sharper. Isoleucine/Leucine were separated with Resolution > 1,5 in all experiments. Pareto’s graphic showed that pH was the only significant factor. From experimental data an optimization was made and was achieved a critical pair resolution of R > 1,6. The optimized conditions was: pH = 3,444; 140,7 mM sodium acetate; and 16,8 mM TEA. The method was selective in relation to Asparagine, Aspartic Acid, Glutamic Acid and Cysteine, and could be evaluated as internal standards for quantification. AQC derivatization products shown to be stable for more than 3 weeks for a qualitative analysis. Conclusion: The method developed shown to be ideal for quality control analysis considering its short derivatization step with a long product stability, speed and robustness for qualitative analysis, and the potential for quantify pure and mixed amino acid. This method is now ready to be validated and implemented at Bio-Manguinhos physicochemical quality control routine.","PeriodicalId":8089,"journal":{"name":"Annals of the symposium: vaccines, biopharmaceuticals, in vitro diagnosis, management, other related themes","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79182058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V. Rhodes, Ana Ajorio, A. Rodrigues, Luciana Costa, R. Miranda
{"title":"Virucidal activity evaluation of hydrogen peroxide wipes against the AZD1222-chimpanzee adenovirus in active pharmaceutical ingredient and the recombinant COVID-19 vaccine","authors":"V. Rhodes, Ana Ajorio, A. Rodrigues, Luciana Costa, R. Miranda","doi":"10.35259/isi.2022_52270","DOIUrl":"https://doi.org/10.35259/isi.2022_52270","url":null,"abstract":"","PeriodicalId":8089,"journal":{"name":"Annals of the symposium: vaccines, biopharmaceuticals, in vitro diagnosis, management, other related themes","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76276255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Patrícia Dalmina de Oliveira, R. Pedro, L. Lignani, G. Teixeira, C. Cordeiro
{"title":"Adverse event profile of ChAdOx1 nCoV-19 vaccine (AZD1222) in Brazil: Comparison between Bio-Manguinhos/Fiocruz post-marketing surveillance database and clinical trials","authors":"Patrícia Dalmina de Oliveira, R. Pedro, L. Lignani, G. Teixeira, C. Cordeiro","doi":"10.35259/isi.2022_52199","DOIUrl":"https://doi.org/10.35259/isi.2022_52199","url":null,"abstract":"","PeriodicalId":8089,"journal":{"name":"Annals of the symposium: vaccines, biopharmaceuticals, in vitro diagnosis, management, other related themes","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87477208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I. Horbach, Renata Santos, M. Pereira, Ivanildo Pedro Sousa Junior, V. Rocha
{"title":"Evaluation of Humoral Response to SARS-CoV-2 after Two-Doses of the ChAdOx1 nCoV-19 Vaccine (Astrazeneca) in a Cohort from Rio de Janeiro, Brazil","authors":"I. Horbach, Renata Santos, M. Pereira, Ivanildo Pedro Sousa Junior, V. Rocha","doi":"10.35259/isi.2022_52257","DOIUrl":"https://doi.org/10.35259/isi.2022_52257","url":null,"abstract":"","PeriodicalId":8089,"journal":{"name":"Annals of the symposium: vaccines, biopharmaceuticals, in vitro diagnosis, management, other related themes","volume":"36 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89128656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Luca Andrade, Aline Albuquerque, Andrielly Costa, Disraeli Vasconcelos, G. Sartori
and reorganizing the side chain of R86 and E84. This conformation change has a prohibitive effect on the interaction between PD-1 PD-L1 given the collision between the F strand, the FG loop, and the N-terminal residues of PD-L1 with the C’D loop of PD-1. Conclusion: Our results reveal a new conformation in PD-1 that prohibits interaction with PD-L1 and can be used as a reference for the formulation of alternative mAbs to block the interaction between the two proteins. From here, we are able, supported by the structural information obtained, to start prospecting for new antibodies targeting the PD-1 and PD-L1 pathway.
{"title":"Discovery and characterization of sites through molecular dynamics with probes and virtual screening to propose new immunobiological targeting the PD-1","authors":"Luca Andrade, Aline Albuquerque, Andrielly Costa, Disraeli Vasconcelos, G. Sartori","doi":"10.35259/isi.2022_52287","DOIUrl":"https://doi.org/10.35259/isi.2022_52287","url":null,"abstract":"and reorganizing the side chain of R86 and E84. This conformation change has a prohibitive effect on the interaction between PD-1 PD-L1 given the collision between the F strand, the FG loop, and the N-terminal residues of PD-L1 with the C’D loop of PD-1. Conclusion: Our results reveal a new conformation in PD-1 that prohibits interaction with PD-L1 and can be used as a reference for the formulation of alternative mAbs to block the interaction between the two proteins. From here, we are able, supported by the structural information obtained, to start prospecting for new antibodies targeting the PD-1 and PD-L1 pathway.","PeriodicalId":8089,"journal":{"name":"Annals of the symposium: vaccines, biopharmaceuticals, in vitro diagnosis, management, other related themes","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89613305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Evandro Dias, A. Durans, B. Succar, P. Pêgo, S. Simone
{"title":"Prototype of an ELISA on the HRP system using a chimeric polyprotein for the diagnosis of chronic Trypanosoma cruzi infection","authors":"Evandro Dias, A. Durans, B. Succar, P. Pêgo, S. Simone","doi":"10.35259/isi.2022_52174","DOIUrl":"https://doi.org/10.35259/isi.2022_52174","url":null,"abstract":"","PeriodicalId":8089,"journal":{"name":"Annals of the symposium: vaccines, biopharmaceuticals, in vitro diagnosis, management, other related themes","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89249360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tamiris A C Barros, Daniele Cunha, J. Melgaço, L. Tubarão, Jane Silva
{"title":"The cellular response elicited by ChAdOx1 nCoV-19 (Astrazeneca) vaccine in a cohort from Rio de Janeiro, Brazil with or without previous SARS-CoV-2 infection","authors":"Tamiris A C Barros, Daniele Cunha, J. Melgaço, L. Tubarão, Jane Silva","doi":"10.35259/isi.2022_52206","DOIUrl":"https://doi.org/10.35259/isi.2022_52206","url":null,"abstract":"","PeriodicalId":8089,"journal":{"name":"Annals of the symposium: vaccines, biopharmaceuticals, in vitro diagnosis, management, other related themes","volume":"418 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79494556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. Assis, Priscilla Soares, P. Martins, C. Corsini, S. Gomes
{"title":"Analysis of vaccine efficacy against COVID-19 in oncology patients under active treatmen","authors":"J. Assis, Priscilla Soares, P. Martins, C. Corsini, S. Gomes","doi":"10.35259/isi.2022_52285","DOIUrl":"https://doi.org/10.35259/isi.2022_52285","url":null,"abstract":"","PeriodicalId":8089,"journal":{"name":"Annals of the symposium: vaccines, biopharmaceuticals, in vitro diagnosis, management, other related themes","volume":"33 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85471939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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