{"title":"Preparation of .BETA. Chain Dominant Collagen of Bovine Achilles Tendon with Pepsin.","authors":"M. Wada, Naohito Koremura, T. Hasegawa","doi":"10.1271/NOGEIKAGAKU1924.74.775","DOIUrl":null,"url":null,"abstract":"牛アキレス腱を10% NaOHによる4°C, 12時間部分加水分解物を洗浄して得られた不溶性残渣から中性塩および酸可溶性コラーゲンを徹底的に除去した.これをペプシン処理してペプシン可溶控コラーゲンを得た.このペプシン可溶性コラーゲンをSephadex G-25, Superdex 200によるゲルろ過クロマグラフィーにより脱塩・分離を行いβ鎖近似コラーゲンを約30%得た.","PeriodicalId":9443,"journal":{"name":"Bulletin of the Agricultural Chemical Society of Japan","volume":"59 1","pages":"775-779"},"PeriodicalIF":0.0000,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bulletin of the Agricultural Chemical Society of Japan","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1271/NOGEIKAGAKU1924.74.775","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
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