Analysis of genetic diversity of passion fruit (Passiflora edulis Sims) genotypes grown in Kenya by sequence-related amplified polymorphism (SRAP) markers

Peter Oluoch , Evans N. Nyaboga , Joel L. Bargul
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引用次数: 9

Abstract

Passion fruit (Passiflora edulis [Sims]) is currently ranked third among fruit exports from Kenya and has great potential since demand for both fresh fruit and processed juice is on a continuous increase. Although assessment of genetic variability of germplasm is indispensable for improvement and development of superior cultivars, little information is available on the genetic diversity of passion fruit cultivated in Kenya. The objective of this study was to determine the genetic diversity of passion fruit genotypes from major growing regions in Kenya using sequence-related amplified polymorphism (SRAP) markers. Twenty four SRAP primer combinations were screened using three passion fruit genotypes and only seven that displayed polymorphic and stable amplification profiles were used to analyze 22 genotypes. The seven primer combinations amplified a total of 931 clear bands with an average of 133 bands per primer pair, of which 610 (65.5%) bands were polymorphic. The similarity coefficients among the 22 passion fruit germplasms ranged from 0.51 to 1.0 with an average of 0.755. The 22 passion fruit genotypes were classified into two groups by cluster analysis using unweighted pair-group method with arithmetic mean (UPGMA) with 12% similarity. Shannon's diversity index was 0.0934 and Nei's gene diversity index was 0.1370 in the present study. The study findings demonstrate the existence of a considerable amount of genetic variability among passion fruit genotypes grown in different regions of Kenya. This indicates the potential application of these genotypes in breeding programs by exploiting the use of molecular markers for selection of specific agronomic traits.

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利用序列相关扩增多态性(SRAP)标记分析肯尼亚百香果基因型的遗传多样性
百香果(Passiflora edulis [Sims])目前在肯尼亚水果出口中排名第三,由于对新鲜水果和加工果汁的需求持续增长,百香果具有巨大的潜力。虽然种质遗传变异的评估对优良品种的改良和开发是必不可少的,但关于肯尼亚栽培的百香果遗传多样性的资料很少。本研究的目的是利用序列相关扩增多态性(SRAP)标记确定肯尼亚主要产区百香果基因型的遗传多样性。从3个百香果基因型中筛选出24个SRAP引物组合,22个基因型中只有7个表现出多态性和稳定的扩增谱。7个引物组合共扩增出931条清晰条带,平均每对引物扩增出133条,其中610条(65.5%)为多态性条带。22份百香果种质间的相似系数为0.51 ~ 1.0,平均为0.755。采用UPGMA聚类分析方法将22个百香果基因型分为两组,相似度为12%。Shannon的基因多样性指数为0.0934,Nei的基因多样性指数为0.1370。研究结果表明,在肯尼亚不同地区种植的百香果基因型之间存在相当数量的遗传变异。这表明这些基因型在利用分子标记选择特定农艺性状方面具有潜在的育种应用前景。
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