Evaluation of Radical Scavenging Activity of Ethnomedicinal Syrup “Nitya Yevvana Kashayam”

D. Sripriya, M. C. Shekara
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引用次数: 1

Abstract

This study aimed to evaluate the antioxidant compounds and their free radical scavenging capacity from Crude aqueous extract of ethnomedicated syrup “ Nitya Yevvana Kashayam (NYK)”. Estimation of antioxidant activity compounds viz ., Total Phenolic Content (TPC), Total Flavonoids (TF) and Flavonol content (T.fl) were optimized by calorimetric methods respectively. DPPH and ABTS scavenging assay were used for the determination of free radical scavenging potentiality. Results reported that TPC (272.30 ± 0.44 mg GAE/gm DW), TF (158.65 ±0.82 mg QCE/gm DW) and the radical scavenging activity measurement, done with DPPH assay (EC 50 = 22.24 ± 0.18 µg/mL) and ABTS assay (EC 50 = 18.64 ± 1.35 µg/mL), present in NYK proved to be a good source of natural antiradical scavenger and therefore, recommended for utilization as potent medicine supporting the traditional ethnic claim.
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民族药糖浆“山茱萸”清除自由基活性的评价
本研究旨在评价民族药糖浆“Nitya yevana Kashayam”(NYK)粗水提物的抗氧化成分及其清除自由基的能力。分别用量热法优化了抗氧化活性化合物总酚含量(TPC)、总黄酮含量(TF)和黄酮醇含量(T.fl)的测定方法。采用DPPH和ABTS清除率测定自由基清除率。结果表明,山楂中TPC(272.30±0.44 mg GAE/gm DW)、TF(158.65±0.82 mg QCE/gm DW)和DPPH (ec50 = 22.24±0.18µg/mL)和ABTS (ec50 = 18.64±1.35µg/mL)的自由基清除活性测定证明,山楂中TPC是一种良好的天然抗自由基清除剂,可作为民族传统主张的有效药物利用。
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