Evaluation of the possibility of hypothermic and subornorthomeric storage of placental mesenchymal stem cells in alginate carriers

Abstract

Mesenchymal stem cells (MSCs) are actively used in regenerative medicine to treat neuropa -thies, diabetes mellitus, burns, ulcers, infertility, premature conditions are able to maintain metabolism at a level sufficient for survival. The morphological study of cells in alginate microspheres was not informative, because of the difficulty cells visualizing in a three-dimensional structure and the density of alginate. Rapid screening tests may include trypan blue staining and MTT test. Conclusions. Placental cells in alginate microsphers can be stored in subnormothermic (+20°C) conditions no more than 48 hours. Storage more than 48 hours in subnormothermic conditions, or 24 hours in hypothermic conditions leads to a sharp metabolic activity decreasing.