Pub Date : 2024-05-12DOI: 10.25040/ecpb2024.01.028
O. A. Nakonechna, V. Y. Prokopyuk, О. S. Sydorenko, V. S. Hoidina, D. O. Yankovska
The article analyzes the effect of different concentrations of GdYVO4 :Eu3+ nanoparticles solution, which were pre-activated by ultraviolet light and not activated, on metabolic activity of the MCF-7 cancer cell culture. It was found that the degree of changes in the state of breast cancer cells depended on the concentration of nanoparticles, namely, the toxic effect was observed under conditions of exposure to high concentrations.
{"title":"The effect of different concentrations of yttrium gadolinium orthovanadate nanoparticles on breast cancer cell line MCF-7","authors":"O. A. Nakonechna, V. Y. Prokopyuk, О. S. Sydorenko, V. S. Hoidina, D. O. Yankovska","doi":"10.25040/ecpb2024.01.028","DOIUrl":"https://doi.org/10.25040/ecpb2024.01.028","url":null,"abstract":"The article analyzes the effect of different concentrations of GdYVO4 :Eu3+ nanoparticles solution, which were pre-activated by ultraviolet light and not activated, on metabolic activity of the MCF-7 cancer cell culture. It was found that the degree of changes in the state of breast cancer cells depended on the concentration of nanoparticles, namely, the toxic effect was observed under conditions of exposure to high concentrations.","PeriodicalId":12101,"journal":{"name":"Experimental and Clinical Physiology and Biochemistry","volume":"108 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140986723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-12DOI: 10.25040/ecpb2024.01.040
O. A. Nakonechna, O. Kyslov
At present, more than 30 types of polypropylene surgical meshes are available in the world, but the statistics regarding the secondary inflammatory process after implantation still remain disappointing. Since polypropylene surgical meshes do not decompose well in the body, they can stimulate the surrounding tissuesto develop an inflammatory process that causes adhesions. For the past 50 years, tantalum has been successfully used to produce biocompatible medical implants in surgery, orthopedics, and dentistry. Its excellent anti-inflammatory and antibacterial properties have been repeatedly noted, indicating the possibility of its use as a coating for mesh implants. In order to better predict the outcome after implantation of biomedical materials, it is important to determine the content of acute phase proteins, namely C-reactive protein, haptoglobin and ceruloplasmin. The aim of the study is to determine the content of acute phase proteins, namely C-reactive protein, haptoglobin and ceruloplasmin in the blood of rats after implantation of surgical meshes coated on the basis of tantalum, tantalum oxide and tantalum nitride. Materials and methods. The experimental group included 40 male rats of the WAG population. With the help of surgical intervention, a polypropylene surgical mesh measuring 15x15 mm was implanted between the abdominal wall and various sections of the colon. 28 days after surgery, the experimental animals were decapitated by cervical dislocation. Blood samples were used to determine the content of C-reactive protein, the content of haptoglobin and the content of ceruloplasmin. Results and discussion. The content of C-reactive protein in the blood of rats was statistically 260 % higher in the experimental group implanted with uncoated surgical mesh and 228.8 % higher in the experimental group implanted with polypropylene surgical mesh with tantalum nitride coating in compared with the results in the group of intact animals. Haptoglobin content was statistically 110.5 % higher and ceruloplasmin was statistically 52.6 % higher in the uncoated surgical mesh group compared to the results of the intact animal group. We obtained similar results in the experimental group, which was implanted with a polypropylene surgical mesh with a coating based on tantalum nitride. The content of haptoglobin was 130 % higher and the content of ceruloplasmin was 50.6 % higher statistically compared to the results of the group of intact animals. The C-reactive protein content was 113.3 % and 95.5 % higher, respectively, in the groups implanted with polypropylene surgical meshes with a coating based on tantalum and tantalum oxide compared to the results in the group of intact animals. The content of haptoglobin in the tantalum-based coating group was 83.8 % higher and the content of ceruloplasmin was 32.6 % higher compared to the results obtained in the group of intact animals. In the tantalum oxide- based group, the haptoglobin content was 60.1 % higher a
目前，世界上有 30 多种聚丙烯手术网，但有关植入后继发炎症过程的统计数据仍然令人失望。由于聚丙烯手术网片在体内不能很好地分解，因此会刺激周围组织发生炎症过程，导致粘连。在过去的 50 年中，钽已被成功地用于生产外科、整形外科和牙科的生物相容性医疗植入物。其卓越的消炎和抗菌特性已被反复提及，这表明它有可能用作网状植入物的涂层。为了更好地预测生物医学材料植入后的效果，确定急性期蛋白（即 C 反应蛋白、血红蛋白和脑磷脂）的含量非常重要。本研究的目的是测定大鼠植入以钽、氧化钽和氮化钽为基础涂层的手术网片后血液中急性期蛋白（即 C 反应蛋白、高铁血红蛋白和脑磷脂）的含量。材料和方法实验组包括 40 只雄性 WAG 大鼠。在外科手术的帮助下，将 15x15 毫米的聚丙烯手术网植入腹壁和结肠的不同部分之间。手术 28 天后，通过颈椎脱位将实验动物斩首。血液样本用于测定 C 反应蛋白含量、高铁血红蛋白含量和脑磷脂含量。结果与讨论据统计，植入无涂层手术网片的实验组大鼠血液中的 C 反应蛋白含量比完整动物组高 260%，植入带氮化钛涂层聚丙烯手术网片的实验组大鼠血液中的 C 反应蛋白含量比完整动物组高 228.8%。据统计，与完整动物组的结果相比，未涂层手术网片组的aptoglobin 含量高 110.5 %，ceruloplasmin 含量高 52.6 %。我们在实验组也得到了类似的结果，实验组植入了带有氮化钛涂层的聚丙烯手术网。与完整动物组的结果相比，血红蛋白含量高出 130%，脑磷脂含量高出 50.6%。与完整动物组的结果相比，植入带有钽和氧化钽涂层的聚丙烯手术网的动物组的 C 反应蛋白含量分别高出 113.3 % 和 95.5 %。与完整动物组的结果相比，钽涂层组的血红蛋白含量高出 83.8%，脑磷脂含量高出 32.6%。与完整动物组的结果相比，氧化钽涂层组的高铁血红蛋白含量高出 60.1%，脑磷脂含量高出 29.3%。结论根据研究结果，涂有钽和氧化钽的聚丙烯手术用网格与未涂网格和涂有氮化钽的网格相比，能显著减轻炎症反应。关键炎症标志物水平的降低也证明了这一点，表明钽和氧化钽涂层的生物相容性和抗炎效果得到了改善。
{"title":"Determination of acute phase proteins in the blood of rats after implantation of polypropylene surgical mesh with a coating based on tantalum and its derivatives","authors":"O. A. Nakonechna, O. Kyslov","doi":"10.25040/ecpb2024.01.040","DOIUrl":"https://doi.org/10.25040/ecpb2024.01.040","url":null,"abstract":"At present, more than 30 types of polypropylene surgical meshes are available in the world, but the statistics regarding the secondary inflammatory process after implantation still remain disappointing. Since polypropylene surgical meshes do not decompose well in the body, they can stimulate the surrounding tissuesto develop an inflammatory process that causes adhesions. For the past 50 years, tantalum has been successfully used to produce biocompatible medical implants in surgery, orthopedics, and dentistry. Its excellent anti-inflammatory and antibacterial properties have been repeatedly noted, indicating the possibility of its use as a coating for mesh implants. In order to better predict the outcome after implantation of biomedical materials, it is important to determine the content of acute phase proteins, namely C-reactive protein, haptoglobin and ceruloplasmin. The aim of the study is to determine the content of acute phase proteins, namely C-reactive protein, haptoglobin and ceruloplasmin in the blood of rats after implantation of surgical meshes coated on the basis of tantalum, tantalum oxide and tantalum nitride. Materials and methods. The experimental group included 40 male rats of the WAG population. With the help of surgical intervention, a polypropylene surgical mesh measuring 15x15 mm was implanted between the abdominal wall and various sections of the colon. 28 days after surgery, the experimental animals were decapitated by cervical dislocation. Blood samples were used to determine the content of C-reactive protein, the content of haptoglobin and the content of ceruloplasmin. Results and discussion. The content of C-reactive protein in the blood of rats was statistically 260 % higher in the experimental group implanted with uncoated surgical mesh and 228.8 % higher in the experimental group implanted with polypropylene surgical mesh with tantalum nitride coating in compared with the results in the group of intact animals. Haptoglobin content was statistically 110.5 % higher and ceruloplasmin was statistically 52.6 % higher in the uncoated surgical mesh group compared to the results of the intact animal group. We obtained similar results in the experimental group, which was implanted with a polypropylene surgical mesh with a coating based on tantalum nitride. The content of haptoglobin was 130 % higher and the content of ceruloplasmin was 50.6 % higher statistically compared to the results of the group of intact animals. The C-reactive protein content was 113.3 % and 95.5 % higher, respectively, in the groups implanted with polypropylene surgical meshes with a coating based on tantalum and tantalum oxide compared to the results in the group of intact animals. The content of haptoglobin in the tantalum-based coating group was 83.8 % higher and the content of ceruloplasmin was 32.6 % higher compared to the results obtained in the group of intact animals. In the tantalum oxide- based group, the haptoglobin content was 60.1 % higher a","PeriodicalId":12101,"journal":{"name":"Experimental and Clinical Physiology and Biochemistry","volume":"118 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140986320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-12DOI: 10.25040/ecpb2024.01.055
O. Dulo
Anaerobic productivity is characterized by the body’s ability to perform muscle work due to anaerobic processes of energy supply and is characterized by their power and capacity. Data on the age-related dynamics of the body’s anaerobic productivity are contradictory. Studying the anaerobic capabilities of girls in the mountainous regions of Zakarpattia Oblast, we understand that the somatotype and functional capabilities of the body are formed in this category of persons in conditions of relative hypoxia. Szymczak R.K. et al researching the effect on the physiology of the body of a long stay at a very high altitude (over 3500 m), male climbers note that in conditions of hypoxia, the anaerobic productivity of the body decreases, the maximum respiratory volume increases, and the relative content of the fat component of the body weight decreases. Therefore, the study of indicators of anaerobic productivity of the body’s energy supply in healthy girls may allow developing individual and population medical prognosis in the future. The level of physical health was assessed by indicators of anaerobic productivity of the body, assessing the ability of women living in mountainous regions to adapt to performing physical work in an anaerobic mode. For this purpose, we used the Wingat anaerobic test method described by Yu.M. Furman et al, the method of Shogy A., Cherebetin G. and somatotype was determined according to Heath-Carter. Having studied the anaerobic capabilities of 102 girls aged 16 to 20, residents of the mountainous regions of the Transcarpathian region. we noted that the performance of physical work in the anaerobic regime of the body’s energy supply depends on the somatotype. The power of anaerobic alactate and lactate processes turned out to be the highest in girls with an endomesomorphic somatotype. The power of anaerobic alactate and lactate processes turned out to be the lowest in girls with an ectomorphic somatotype. Studying the level of anaerobic productivity of females based on the relative indicator of the capacity of anaerobic lactate processes of energy supply showed that girls with a balanced somatotype have the lowest level of anaerobic capacity compared to girls of other somatotypes.
{"title":"Anaerobic capaсity of female residents of the mountainous districts of Zakarpattia in the postpubertal period of ontogenesis","authors":"O. Dulo","doi":"10.25040/ecpb2024.01.055","DOIUrl":"https://doi.org/10.25040/ecpb2024.01.055","url":null,"abstract":"Anaerobic productivity is characterized by the body’s ability to perform muscle work due to anaerobic processes of energy supply and is characterized by their power and capacity. Data on the age-related dynamics of the body’s anaerobic productivity are contradictory. Studying the anaerobic capabilities of girls in the mountainous regions of Zakarpattia Oblast, we understand that the somatotype and functional capabilities of the body are formed in this category of persons in conditions of relative hypoxia. Szymczak R.K. et al researching the effect on the physiology of the body of a long stay at a very high altitude (over 3500 m), male climbers note that in conditions of hypoxia, the anaerobic productivity of the body decreases, the maximum respiratory volume increases, and the relative content of the fat component of the body weight decreases. Therefore, the study of indicators of anaerobic productivity of the body’s energy supply in healthy girls may allow developing individual and population medical prognosis in the future. The level of physical health was assessed by indicators of anaerobic productivity of the body, assessing the ability of women living in mountainous regions to adapt to performing physical work in an anaerobic mode. For this purpose, we used the Wingat anaerobic test method described by Yu.M. Furman et al, the method of Shogy A., Cherebetin G. and somatotype was determined according to Heath-Carter. Having studied the anaerobic capabilities of 102 girls aged 16 to 20, residents of the mountainous regions of the Transcarpathian region. we noted that the performance of physical work in the anaerobic regime of the body’s energy supply depends on the somatotype. The power of anaerobic alactate and lactate processes turned out to be the highest in girls with an endomesomorphic somatotype. The power of anaerobic alactate and lactate processes turned out to be the lowest in girls with an ectomorphic somatotype. Studying the level of anaerobic productivity of females based on the relative indicator of the capacity of anaerobic lactate processes of energy supply showed that girls with a balanced somatotype have the lowest level of anaerobic capacity compared to girls of other somatotypes.","PeriodicalId":12101,"journal":{"name":"Experimental and Clinical Physiology and Biochemistry","volume":"102 34","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140987488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-12DOI: 10.25040/ecpb2024.01.005
I. Fomenko, T. Bondarchuk
Hydrogen sulfide (H2S) belongs to the family of «gasotransmitters» can by synthesized by enzymatic systems and also formed non-enzymatically. At physiological concentrations, it regulates a range of biological functions in various organs and tissues. H2 S is involved in biochemical changes that play an important role in the pathogenesis of diseases such as cancer, COVID-19, diabetes mellitus, and neurodegenerative pathologies. In carcinogenesis, H2S influences cancer cell proliferation, inhibits cancer cell apoptosis, regulates the cell cycle, intracellular signaling pathways, stimulates angiogenesis, and autophagy of cancer cells. In lung inflammation caused by COVID-19, H2S disrupts disulfide bonds in mucus, reducing its viscosity, blocks NF-κB pathway activation, preventing the onset of a «cytokine storm», promotes Nrf2 activation, increasing the expression of antioxidant molecules and enzymes, activates potassium channels, and blocks Na+/K+-ATPase, promoting electrolyte absorption. In the pancreas, H 2 S regulates insulin secretion and plays a significant role in insulin sensitivity regulation in insulin-responsive tissues. It inhibits glucose uptake and glycogen accumulation, which is crucial in diabetes mellitus. In adipose tissue, H 2 S promotes adipogenesis, inhibits lipolysis, and regulates the secretion of adiponectin and MCP-1 in type 2 diabetes. In neural tissue, H2S acts as a neuromodulator, increases GABA expression, induces Ca2+ concentration increase, participates in long-term potentiation, neurotransmitter modulation, affects NADPH levels, and exerts epigenetic effects. Understanding the role of H2 S may be crucial in developing effective therapy strategies for various diseases.
硫化氢（H2S）属于 "气体递质 "家族，可以由酶系统合成，也可以非酶生成。在生理浓度下，它能调节各种器官和组织的一系列生物功能。H2 S 参与生化变化，在癌症、COVID-19、糖尿病和神经退行性病变等疾病的发病机制中发挥重要作用。在致癌过程中，H2S 影响癌细胞增殖，抑制癌细胞凋亡，调节细胞周期、细胞内信号通路，刺激血管生成和癌细胞自噬。在 COVID-19 引起的肺部炎症中，H2S 可破坏粘液中的二硫键，降低粘度；阻断 NF-κB 通路的激活，防止 "细胞因子风暴 "的发生；促进 Nrf2 的激活，增加抗氧化分子和酶的表达；激活钾通道；阻断 Na+/K+-ATP 酶，促进电解质的吸收。在胰腺中，H 2 S 可调节胰岛素分泌，并在胰岛素反应组织的胰岛素敏感性调节中发挥重要作用。它能抑制葡萄糖摄取和糖原累积，这对糖尿病至关重要。在脂肪组织中，H 2 S 促进脂肪生成，抑制脂肪分解，调节 2 型糖尿病患者体内脂肪连素和 MCP-1 的分泌。在神经组织中，H2 S 可作为神经调节剂，增加 GABA 的表达，诱导 Ca2+ 浓度增加，参与长期电位、神经递质调节，影响 NADPH 水平，并发挥表观遗传效应。了解 H2 S 的作用可能对开发治疗各种疾病的有效策略至关重要。
{"title":"Hydrogen sulfide: biological and pathochemistry","authors":"I. Fomenko, T. Bondarchuk","doi":"10.25040/ecpb2024.01.005","DOIUrl":"https://doi.org/10.25040/ecpb2024.01.005","url":null,"abstract":"Hydrogen sulfide (H2S) belongs to the family of «gasotransmitters» can by synthesized by enzymatic systems and also formed non-enzymatically. At physiological concentrations, it regulates a range of biological functions in various organs and tissues. H2 S is involved in biochemical changes that play an important role in the pathogenesis of diseases such as cancer, COVID-19, diabetes mellitus, and neurodegenerative pathologies. In carcinogenesis, H2S influences cancer cell proliferation, inhibits cancer cell apoptosis, regulates the cell cycle, intracellular signaling pathways, stimulates angiogenesis, and autophagy of cancer cells. In lung inflammation caused by COVID-19, H2S disrupts disulfide bonds in mucus, reducing its viscosity, blocks NF-κB pathway activation, preventing the onset of a «cytokine storm», promotes Nrf2 activation, increasing the expression of antioxidant molecules and enzymes, activates potassium channels, and blocks Na+/K+-ATPase, promoting electrolyte absorption. In the pancreas, H 2 S regulates insulin secretion and plays a significant role in insulin sensitivity regulation in insulin-responsive tissues. It inhibits glucose uptake and glycogen accumulation, which is crucial in diabetes mellitus. In adipose tissue, H 2 S promotes adipogenesis, inhibits lipolysis, and regulates the secretion of adiponectin and MCP-1 in type 2 diabetes. In neural tissue, H2S acts as a neuromodulator, increases GABA expression, induces Ca2+ concentration increase, participates in long-term potentiation, neurotransmitter modulation, affects NADPH levels, and exerts epigenetic effects. Understanding the role of H2 S may be crucial in developing effective therapy strategies for various diseases.","PeriodicalId":12101,"journal":{"name":"Experimental and Clinical Physiology and Biochemistry","volume":"104 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140987228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-12DOI: 10.25040/ecpb2024.01.032
N. R. Hrytsevich, V. V. Vereschaka
It is known that metabolic syndrome (MS) prolongs the healing time of the wound surface of various genesis. We hypothesized that this may be related to changes in the biophysical properties of the skin. The aim of the work was to investigate the biophysical properties of skin (content of moisture, collagen, fused gelatin in the skin, and skin thermal fusion temperature) in rats with MS. Materials and methods. Research was conducted on 40 white non-linear rats (20 – males, 20 – females). After birth, males and females were divided into 2 groups. The first group served as a control for the second groups, in which MS was simulated by administering monosodium glutamate at a dose of 4.0 mg/kg on the 2nd, 4th, 6th, 8th, and 10th postnatal days. At the age of 4 months, the animals were decapitated under ether anesthesia. Biochemical studies were conducted to confirm the development of MS. The percentage of moisture, collagen content, destructive changes in collagen fibers and resistance of skin collagen to thermal factors were determined in native skin. The results. 4-month-old rats injected with monosodium glutamate in the early neonatal period developed MS as evidenced by abdominal obesity, insulin resistance, and dyslipidemia. In comparison with animals of the control group, in the skin of male and female rats with MS, the moisture content decreased, and the collagen content did not change. At the same time, the percentage of melted gelatin doubled, which indicates the depth of destructive changes in collagen fibers. Thermal welding of skin samples of male and female rats with signs of MS occurred at rather low temperatures compared to rats of the control group. Conclusion. Changes in the biophysical properties of the skin, which are registered in rats with MS, may be the reason for the later closure of the wound surface.
{"title":"Biophysical properties of the skin of rats with metabolic syndrome","authors":"N. R. Hrytsevich, V. V. Vereschaka","doi":"10.25040/ecpb2024.01.032","DOIUrl":"https://doi.org/10.25040/ecpb2024.01.032","url":null,"abstract":"It is known that metabolic syndrome (MS) prolongs the healing time of the wound surface of various genesis. We hypothesized that this may be related to changes in the biophysical properties of the skin. The aim of the work was to investigate the biophysical properties of skin (content of moisture, collagen, fused gelatin in the skin, and skin thermal fusion temperature) in rats with MS. Materials and methods. Research was conducted on 40 white non-linear rats (20 – males, 20 – females). After birth, males and females were divided into 2 groups. The first group served as a control for the second groups, in which MS was simulated by administering monosodium glutamate at a dose of 4.0 mg/kg on the 2nd, 4th, 6th, 8th, and 10th postnatal days. At the age of 4 months, the animals were decapitated under ether anesthesia. Biochemical studies were conducted to confirm the development of MS. The percentage of moisture, collagen content, destructive changes in collagen fibers and resistance of skin collagen to thermal factors were determined in native skin. The results. 4-month-old rats injected with monosodium glutamate in the early neonatal period developed MS as evidenced by abdominal obesity, insulin resistance, and dyslipidemia. In comparison with animals of the control group, in the skin of male and female rats with MS, the moisture content decreased, and the collagen content did not change. At the same time, the percentage of melted gelatin doubled, which indicates the depth of destructive changes in collagen fibers. Thermal welding of skin samples of male and female rats with signs of MS occurred at rather low temperatures compared to rats of the control group. Conclusion. Changes in the biophysical properties of the skin, which are registered in rats with MS, may be the reason for the later closure of the wound surface.","PeriodicalId":12101,"journal":{"name":"Experimental and Clinical Physiology and Biochemistry","volume":"109 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140986766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-12DOI: 10.25040/ecpb2024.01.020
O. V. Babenko, I. Vasylyeva, N. V. Yarmysh, O. A. Nakonechna
The high frequency of prevalence and the decrease in the quality of life of patients determine the urgency of studying the peculiarities of the etiology and pathogenesis of inflammatory bowel diseases. The difficulty of finding new non-invasive markers lies in the lack of a clear understanding of the molecular mechanisms of the pathogenesis of these diseases. One of the promising directions in the diagnosis of chronic inflammatory diseases is the identification of specific miRNA molecules in blood serum, which act as powerful negative regulators of gene expression.
{"title":"Modern ideas about the pathogenetic mechanisms of the development of inflammatory bowel diseases","authors":"O. V. Babenko, I. Vasylyeva, N. V. Yarmysh, O. A. Nakonechna","doi":"10.25040/ecpb2024.01.020","DOIUrl":"https://doi.org/10.25040/ecpb2024.01.020","url":null,"abstract":"The high frequency of prevalence and the decrease in the quality of life of patients determine the urgency of studying the peculiarities of the etiology and pathogenesis of inflammatory bowel diseases. The difficulty of finding new non-invasive markers lies in the lack of a clear understanding of the molecular mechanisms of the pathogenesis of these diseases. One of the promising directions in the diagnosis of chronic inflammatory diseases is the identification of specific miRNA molecules in blood serum, which act as powerful negative regulators of gene expression.","PeriodicalId":12101,"journal":{"name":"Experimental and Clinical Physiology and Biochemistry","volume":"104 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140987625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-12DOI: 10.25040/ecpb2024.01.049
I. Burlaka, I. Mityuryayeva
A wide variety of both calcium-dependent and calcium-non-dependent actions are attributed to the vitamin and hormone vitamin D. One of the most vital components of the human body, vitamin D is essential to both health and illness. It is a member of the fat-soluble secosteroid family, which is obtained from diets or direct sun exposure, which is what turns 7-hydroxycholesterol into the precursor of vitamin D. Bio-activation is an alternate phase that provides an active version of vitamin D that contributes to several notable processes like detoxification, fertility, glucose regulation, bone remodelling, and calcium regulation. Numerous research investigations examine the discernible function of vitamin D in kidney illness. The basic physiological and pathological roles of vitamin D in the kidneys of both diabetics and non-diabetics were examined in this study. In this study we analyzed a basic physiological and pathological roles of Vitamin D in kidney in terms of diabetic and non-diabetic proteinuric kidney diseases. Further research in this field is of high importance.
维生素 D 是人体最重要的成分之一，对健康和疾病都至关重要。它是脂溶性类固醇家族中的一员，可从饮食或阳光直接照射中获得，它将 7-羟基胆固醇转化为维生素 D 的前体。生物活化是一个交替阶段，它提供了一种活性维生素 D，有助于解毒、生育、葡萄糖调节、骨骼重塑和钙调节等多个显著过程。许多研究调查探讨了维生素 D 在肾脏疾病中的明显功能。本研究探讨了维生素 D 在糖尿病患者和非糖尿病患者肾脏中的基本生理和病理作用。本研究分析了维生素 D 在糖尿病和非糖尿病蛋白尿肾脏疾病中的基本生理和病理作用。该领域的进一步研究具有重要意义。
{"title":"Vitamin D in kidney: a two-edged sword?","authors":"I. Burlaka, I. Mityuryayeva","doi":"10.25040/ecpb2024.01.049","DOIUrl":"https://doi.org/10.25040/ecpb2024.01.049","url":null,"abstract":"A wide variety of both calcium-dependent and calcium-non-dependent actions are attributed to the vitamin and hormone vitamin D. One of the most vital components of the human body, vitamin D is essential to both health and illness. It is a member of the fat-soluble secosteroid family, which is obtained from diets or direct sun exposure, which is what turns 7-hydroxycholesterol into the precursor of vitamin D. Bio-activation is an alternate phase that provides an active version of vitamin D that contributes to several notable processes like detoxification, fertility, glucose regulation, bone remodelling, and calcium regulation. Numerous research investigations examine the discernible function of vitamin D in kidney illness. The basic physiological and pathological roles of vitamin D in the kidneys of both diabetics and non-diabetics were examined in this study. In this study we analyzed a basic physiological and pathological roles of Vitamin D in kidney in terms of diabetic and non-diabetic proteinuric kidney diseases. Further research in this field is of high importance.","PeriodicalId":12101,"journal":{"name":"Experimental and Clinical Physiology and Biochemistry","volume":" 70","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141128784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-30DOI: 10.25040/ecpb2023.03.042
H. S. Dubetska, E. Y. Sklyrov
The article presents the results of own research on the study of the incidence of osteoporosis and osteopenia in men with different levels of uric acid and the influence of hyperuricemia on the mineral density of bone tissue. The aim of the study was to examine the structural and functional state of bone tissue (indicators of mineral density) in men with different levels of uric acid and to establish the incidence of osteoporosis and osteopenia in patients with hyperuricemia. Materials and methods of research. 140 men aged 50–80 years were examined. Determination of the level of uric acid in the serum was performed using the uricase-peroxidase method. Determination of bone mineral density was performed by DXA on the device «Prodigy». Results. It has been established that the incidence of osteoporosis and osteopenia in men with hyperuricemia is lower compared to men with normouricemia. When dividing by age groups 50–59 years, 60–69 years, 70–79 years in men with hyperuricemia, osteoporosis was detected only in the age group 70–79 years and its incidence was 14% at the level of the lumbar spine (L1–L4) and 14% at the level of the femoral neck (Neack Mean). According to the quarterly distribution according to the level of uric acid, men with the highest level of uric acid had the highest indicators of bone mineral density. Conclusions. Patients with a high level of uric acid have high levels of bone mineral density, so they are less likely to develop diseases such as osteoporosis and osteopenia, which indicates the protective role of uric acid for bone tissue and may be of practical importance when deciding on the issue of drug correction of hyperuricemia.
{"title":"Systemic osteoporosis and osteopenia in men with hyperuricemia","authors":"H. S. Dubetska, E. Y. Sklyrov","doi":"10.25040/ecpb2023.03.042","DOIUrl":"https://doi.org/10.25040/ecpb2023.03.042","url":null,"abstract":"The article presents the results of own research on the study of the incidence of osteoporosis and osteopenia in men with different levels of uric acid and the influence of hyperuricemia on the mineral density of bone tissue. The aim of the study was to examine the structural and functional state of bone tissue (indicators of mineral density) in men with different levels of uric acid and to establish the incidence of osteoporosis and osteopenia in patients with hyperuricemia. Materials and methods of research. 140 men aged 50–80 years were examined. Determination of the level of uric acid in the serum was performed using the uricase-peroxidase method. Determination of bone mineral density was performed by DXA on the device «Prodigy». Results. It has been established that the incidence of osteoporosis and osteopenia in men with hyperuricemia is lower compared to men with normouricemia. When dividing by age groups 50–59 years, 60–69 years, 70–79 years in men with hyperuricemia, osteoporosis was detected only in the age group 70–79 years and its incidence was 14% at the level of the lumbar spine (L1–L4) and 14% at the level of the femoral neck (Neack Mean). According to the quarterly distribution according to the level of uric acid, men with the highest level of uric acid had the highest indicators of bone mineral density. Conclusions. Patients with a high level of uric acid have high levels of bone mineral density, so they are less likely to develop diseases such as osteoporosis and osteopenia, which indicates the protective role of uric acid for bone tissue and may be of practical importance when deciding on the issue of drug correction of hyperuricemia.","PeriodicalId":12101,"journal":{"name":"Experimental and Clinical Physiology and Biochemistry","volume":"434 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139203376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-30DOI: 10.25040/ecpb2023.03.005
N. Pokrovska, H. Sklyarova, N. Denysenko, I. Fomenko, E. Sklyarov, L. Kobylinska
Background. The development of the coronavirus disease (COVID-19) occurs against the background of a systemic inflammatory response and can cause an adverse effect on the cardiovascular system. For better diagnosis of disease prognosis, and treatment, determination of sST2 and vWF is import- ant. These markers play an important role in the genesis of thrombosis in the pathophysiology of COVID- 19. Aim of the study. To analyze the prognostic value of sST2 and vWF levels in blood serum of patients with arterial hypertension (AH) and COVID-19. Materials and methods. 71 patients with AH and 16 practically healthy individuals, who were included in the control group, were examined. Patients were divided into two groups: group 1–36 patients with AH who were admit- ted to the hospital with a hypertensive crisis. Group 2 included 35 patients with AH and polysegmental pneumonia on the background of COVID-19. All patients underwent anthropometry, determination of biochemical blood tests, coagulogram, echocardiography (EchoCG), level of sST2 and vWF using ELISA in blood serum. Venous blood sampling was carried out on the 5-7th day after hospitalization against the background of the treatment according to current protocols. Results. The level of sST2 was the highest in the group of patients with AH and COVID-19, while the level of vWF was slightly lower than in patients with AH, which is probably due to the therapy with glucocorticosteroids and low molecular weight heparins. A positive correlation was found between the sST2 level and the size of the left atrium, left ventricle and ejection fraction in patients with AH, while a positive correlation with age was noted in the group of patients with AH and COVID-19 (p < 0.05). Therefore, the level of sST2 was associated with structural changes in the myocardium, which are characteristic of AH and heart failure, which in turn are associated with age-related changes. A positive correlation was established between vWF and the thickness of the posterior wall of the left ventricle, the size of the right ventricle, in patients with AH, while in patients of the group 2, a correlation was found between vWF and the level of leukocytes (p < 0.05). Conclusion. The levels of sST2 and vWF are important markers of disease severity in patients with cardiovascular pathology, as well as COVID-19. In patients with AH and in combination with COVID-19, a significant increase in the concentration of sST2 and vWF was observed compared to practically healthy individuals. Nevertheless, the presence of COVID-19 did not contribute to the emergence of significantly higher values of these markers compared to the group of individuals with AH, which is probably due to the specifics of the infectious pathology treatment.
{"title":"Prognostic value of sST2 and vWF determination in blood serum in patients with arterial hypertension and covid-19","authors":"N. Pokrovska, H. Sklyarova, N. Denysenko, I. Fomenko, E. Sklyarov, L. Kobylinska","doi":"10.25040/ecpb2023.03.005","DOIUrl":"https://doi.org/10.25040/ecpb2023.03.005","url":null,"abstract":"Background. The development of the coronavirus disease (COVID-19) occurs against the background of a systemic inflammatory response and can cause an adverse effect on the cardiovascular system. For better diagnosis of disease prognosis, and treatment, determination of sST2 and vWF is import- ant. These markers play an important role in the genesis of thrombosis in the pathophysiology of COVID- 19. Aim of the study. To analyze the prognostic value of sST2 and vWF levels in blood serum of patients with arterial hypertension (AH) and COVID-19. Materials and methods. 71 patients with AH and 16 practically healthy individuals, who were included in the control group, were examined. Patients were divided into two groups: group 1–36 patients with AH who were admit- ted to the hospital with a hypertensive crisis. Group 2 included 35 patients with AH and polysegmental pneumonia on the background of COVID-19. All patients underwent anthropometry, determination of biochemical blood tests, coagulogram, echocardiography (EchoCG), level of sST2 and vWF using ELISA in blood serum. Venous blood sampling was carried out on the 5-7th day after hospitalization against the background of the treatment according to current protocols. Results. The level of sST2 was the highest in the group of patients with AH and COVID-19, while the level of vWF was slightly lower than in patients with AH, which is probably due to the therapy with glucocorticosteroids and low molecular weight heparins. A positive correlation was found between the sST2 level and the size of the left atrium, left ventricle and ejection fraction in patients with AH, while a positive correlation with age was noted in the group of patients with AH and COVID-19 (p < 0.05). Therefore, the level of sST2 was associated with structural changes in the myocardium, which are characteristic of AH and heart failure, which in turn are associated with age-related changes. A positive correlation was established between vWF and the thickness of the posterior wall of the left ventricle, the size of the right ventricle, in patients with AH, while in patients of the group 2, a correlation was found between vWF and the level of leukocytes (p < 0.05). Conclusion. The levels of sST2 and vWF are important markers of disease severity in patients with cardiovascular pathology, as well as COVID-19. In patients with AH and in combination with COVID-19, a significant increase in the concentration of sST2 and vWF was observed compared to practically healthy individuals. Nevertheless, the presence of COVID-19 did not contribute to the emergence of significantly higher values of these markers compared to the group of individuals with AH, which is probably due to the specifics of the infectious pathology treatment.","PeriodicalId":12101,"journal":{"name":"Experimental and Clinical Physiology and Biochemistry","volume":"95 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139206957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-30DOI: 10.25040/ecpb2023.03.031
O. Dulo, N. M. Hema-Bahyna, P. P. Brekhlichuk
Aerobic and anaerobic productivity of the body are integral indicators of physical health. assessment of physical health based on indicators of aerobic and anaerobic productivity makes it possible to characterize it not only qualitatively, but also quantitatively [3]. The ability to demonstrate aerobic capabilities largely depends on the content of muscles, which are the main consumer of oxygen in the body [10]. Fat does the role of a regulator of metabolic processes in the body and is the main source of energy during long-term work of low intensity, which is performed due to aerobic energy supply mechanisms of muscle activity. To clarify the degree of influence of somatotype components and components of body weight on the functional capabilities of young men living in the mountainous districts of Zakarpattia, we conducted a correlation analysis. Researches was conducted in 124 young men of the post-puberty period of ontogenesis aged 17 to 21 years, residents of the mountainous districts of the Zakarpattia region. Physical health status was assessed by indicators of the aerobic productivity of the body, namely, the maximum oxygen consumption was measured (VО 2 max ) using the bicycle ergometry method. To evaluate the level of aerobic productivity, the Ya. P. Pyarnat’s rating scale was used [14]. Indicators of anaerobic productivity of the body were studied by: measuring the power of anaerobic alactic energy supply processes by the Peak Power Output in 10 s (WAnT 10 ); the power of anaerobic lactic energy supply processes by the Peak Power Output in 30 s (WAnT 30 ), using the Wingate anaerobic test described by Yu. M. Furman et al. [15]. The anaerobic lactic productivity of the organism was measured by the Peak Power Output (PPO) in 1 min using A. Shogy and G. Cherebetin’s method [16]. The component body mass composition was determined using the impedance method with the application of Somatotype was determined by the Heath-Carter method, which provides a three-component (fat, muscle and bone) anthropometric assessment [17]. Omron BF511 Body Composition Monitor to estimate the percentage of fat mass (subcutaneous and visceral fat) and the percentage of skeletal muscle [18]. The statistical processing of the material was carried out in Excel 7.0 and SPSS version 10.0 using Student’s t-test to find out the reliability of the difference between the average values. In young men, divided by somatotype, it was established that in representatives of the endomesomorphic somatotype, higher values of the fat component largely cause lower values of VO 2 max rel. to (r = -0.706; p < 0.001), correspondingly lower level of aerobic performance. Higher values of BMI in young men of endomesomorphic and mesomorphic somatotypes cause higher values of the absolute VO2 max indicator, as indicated by the correlation (r = 0.727; p < 0.001 and r = 0.880; p < 0.001, respectively). The degree of development of the components of the somatotype does not have a significant im
{"title":"Correlations Between the Functional State of the Organism and the Constitutional Characteristics of the Composition of Body Weight Males the Mountain Districts of Zakarpattia","authors":"O. Dulo, N. M. Hema-Bahyna, P. P. Brekhlichuk","doi":"10.25040/ecpb2023.03.031","DOIUrl":"https://doi.org/10.25040/ecpb2023.03.031","url":null,"abstract":"Aerobic and anaerobic productivity of the body are integral indicators of physical health. assessment of physical health based on indicators of aerobic and anaerobic productivity makes it possible to characterize it not only qualitatively, but also quantitatively [3]. The ability to demonstrate aerobic capabilities largely depends on the content of muscles, which are the main consumer of oxygen in the body [10]. Fat does the role of a regulator of metabolic processes in the body and is the main source of energy during long-term work of low intensity, which is performed due to aerobic energy supply mechanisms of muscle activity. To clarify the degree of influence of somatotype components and components of body weight on the functional capabilities of young men living in the mountainous districts of Zakarpattia, we conducted a correlation analysis. Researches was conducted in 124 young men of the post-puberty period of ontogenesis aged 17 to 21 years, residents of the mountainous districts of the Zakarpattia region. Physical health status was assessed by indicators of the aerobic productivity of the body, namely, the maximum oxygen consumption was measured (VО 2 max ) using the bicycle ergometry method. To evaluate the level of aerobic productivity, the Ya. P. Pyarnat’s rating scale was used [14]. Indicators of anaerobic productivity of the body were studied by: measuring the power of anaerobic alactic energy supply processes by the Peak Power Output in 10 s (WAnT 10 ); the power of anaerobic lactic energy supply processes by the Peak Power Output in 30 s (WAnT 30 ), using the Wingate anaerobic test described by Yu. M. Furman et al. [15]. The anaerobic lactic productivity of the organism was measured by the Peak Power Output (PPO) in 1 min using A. Shogy and G. Cherebetin’s method [16]. The component body mass composition was determined using the impedance method with the application of Somatotype was determined by the Heath-Carter method, which provides a three-component (fat, muscle and bone) anthropometric assessment [17]. Omron BF511 Body Composition Monitor to estimate the percentage of fat mass (subcutaneous and visceral fat) and the percentage of skeletal muscle [18]. The statistical processing of the material was carried out in Excel 7.0 and SPSS version 10.0 using Student’s t-test to find out the reliability of the difference between the average values. In young men, divided by somatotype, it was established that in representatives of the endomesomorphic somatotype, higher values of the fat component largely cause lower values of VO 2 max rel. to (r = -0.706; p < 0.001), correspondingly lower level of aerobic performance. Higher values of BMI in young men of endomesomorphic and mesomorphic somatotypes cause higher values of the absolute VO2 max indicator, as indicated by the correlation (r = 0.727; p < 0.001 and r = 0.880; p < 0.001, respectively). The degree of development of the components of the somatotype does not have a significant im","PeriodicalId":12101,"journal":{"name":"Experimental and Clinical Physiology and Biochemistry","volume":"66 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139200747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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