Inter-laboratory validation by event-specific qPCR methods for the detection of genetically modified insect and herbicide-tolerant maize DBN9501

IF 1.4 3区农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Journal of Consumer Protection and Food Safety Pub Date : 2023-07-20 DOI:10.1007/s00003-023-01450-z

Jing Wang, Bing Xiao, Ruiying Zhang, Haitao Guan, Yang Yang, Yijia Ding, Ran Yuan, Xiaolei Zhang, Lingyan Li, Jingang Liang, Hongtao Wen

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Abstract

DBN9501 is a new maize transgenic event characterised by being resistance to insects and herbicides. To meet genetically modified (GM)-labeling requirements and monitor the unintended release of genetically modified organisms (GMOs), developing a creditable and applicable method for identifying and quantifying GM events is essential. Herein we developed an event-specific method and validated it through inter-laboratory ring trials using blind samples. The limit of detection (LOD) and limit of quantification (LOQ) of copy number ratio were confirmed at 0.05% and 0.1%, respectively. The quantitative bias ranged from -3.52 to 10.38%, and the relative standard deviation (RSD) of the method was < 25%. Furthermore, the expanded uncertainty for the blind samples S1 − S5 was 0.22%, 0.10%, 0.05%, 0.03%, and 0.02%. These results demonstrated that the event-specific quantitative PCR method could identify and quantify GM DBN9501 for further routine lab analysis.

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用事件特异性qPCR方法检测转基因抗虫抗除草剂玉米DBN9501的实验室间验证

DBN9501是玉米转基因新事件，具有抗虫、抗除草剂的特点。为了满足转基因(GM)的标签要求和监测转基因生物的意外释放，开发一种可信和适用的方法来识别和量化转基因事件是必不可少的。在此，我们开发了一种特定事件的方法，并通过使用盲样本的实验室间环试验对其进行了验证。样品拷贝数比的检出限和定量限分别为0.05%和0.1%。定量偏差范围为-3.52 ~ 10.38%，方法的相对标准偏差(RSD)为<25%。盲样S1 ~ S5的扩展不确定度分别为0.22%、0.10%、0.05%、0.03%和0.02%。这些结果表明，事件特异性定量PCR方法可以鉴定和定量GM DBN9501，为进一步的常规实验室分析提供依据。

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来源期刊

Journal of Consumer Protection and Food Safety Veterinary-Food Animals

CiteScore

3.70

自引率

4.20%

发文量

审稿时长

>12 weeks

期刊介绍： The JCF publishes peer-reviewed original Research Articles and Opinions that are of direct importance to Food and Feed Safety. This includes Food Packaging, Consumer Products as well as Plant Protection Products, Food Microbiology, Veterinary Drugs, Animal Welfare and Genetic Engineering. All peer-reviewed articles that are published should be devoted to improve Consumer Health Protection. Reviews and discussions are welcomed that address legal and/or regulatory decisions with respect to risk assessment and management of Food and Feed Safety issues on a scientific basis. It addresses an international readership of scientists, risk assessors and managers, and other professionals active in the field of Food and Feed Safety and Consumer Health Protection. Manuscripts – preferably written in English but also in German – are published as Research Articles, Reviews, Methods and Short Communications and should cover aspects including, but not limited to: · Factors influencing Food and Feed Safety · Factors influencing Consumer Health Protection · Factors influencing Consumer Behavior · Exposure science related to Risk Assessment and Risk Management · Regulatory aspects related to Food and Feed Safety, Food Packaging, Consumer Products, Plant Protection Products, Food Microbiology, Veterinary Drugs, Animal Welfare and Genetic Engineering · Analytical methods and method validation related to food control and food processing. The JCF also presents important News, as well as Announcements and Reports about administrative surveillance.