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Guideline for the verification of digital PCR methods in analytical GMO testing 转基因生物分析检测中数字 PCR 方法验证指南
IF 2.4 3区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-06-25 DOI: 10.1007/s00003-024-01516-6
Christopher Weidner, Jakob Frenzel, Daniela Bartsch, Hans-Ulrich Waiblinger, Joachim Mankertz

The international working group “Development of methods for identification of foodstuffs produced by means of genetic engineering techniques” was established pursuant to § 64 of the German Food and Feed Code (LFGB). It has developed a guideline for implementing and verifying digital PCR methods previously validated as real-time PCR techniques. While event-specific methods in testing of genetically modified organisms (GMO) have traditionally relied on real-time PCR, new digital PCR technologies offer significant advantages and therefore, leading to an increased use by control laboratories. This guideline provides practical recommendations for transferring the real-time PCR to digital PCR and for verifying the digital PCR method. The guideline is applicable to analysis of GMO in food, feed and seed and is freely available on the website of the Federal Office of Consumer Protection and Food Safety (BVL).

根据《德国食品和饲料法典》(LFGB)第 64 条,成立了 "基因工程技术食品鉴别方法开发 "国际工作组。该工作组为实施和验证先前作为实时 PCR 技术验证的数字 PCR 方法制定了指导原则。虽然转基因生物 (GMO) 检测中的特定事件检测方法传统上依赖于实时 PCR,但新的数字 PCR 技术具有显著优势,因此控制实验室越来越多地使用这种方法。本指南提供了将实时 PCR 转为数字 PCR 以及验证数字 PCR 方法的实用建议。该指南适用于食品、饲料和种子中转基因生物的分析,可在联邦消费者保护和食品安全办公室 (BVL) 的网站上免费获取。
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引用次数: 0
Comprehensive analysis of 19 cannabinoids in commercial CBD oils: concentrations, profiles, and safety implications 全面分析商用 CBD 油中的 19 种大麻素:浓度、特征和安全影响
IF 2.4 3区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-06-20 DOI: 10.1007/s00003-024-01513-9
Niklas Lindekamp, Stefan Weigel, Benjamin Sachse, Bernd Schäfer, Sascha Rohn, Nadja Triesch

Nineteen cannabinoids, including Δ9-THC (tetrahydrocannabinol) and CBD (cannabidiol), were quantified in 22 CBD oils, 2 CBG (cannabigerol) oils, and 2 CBN (cannabinol) oils, marketed as food supplements, aroma oils, or cosmetic preparations. Analyses were conducted using high-performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS/MS). The declared concentrations of CBD (or CBG, CBN) in the oils ranged from 2.5 to 20%. Actual concentrations compared to declared concentrations ranged from 81 to 226%. CBD concentrations of up to 9 mg per drop were found. Δ9-THC was detected in 20 of 26 samples, with concentrations ranging from 5 to 1576 mg/kg (mean = 536 mg/kg). Considering the highest daily intake suggested by one manufacturer (20 drops) and a body weight of 70 kg, the measured Δ9-THC concentration in 50% (n = 13) of the products would exceed the acute reference dose (ARfD) of 1 µg/kg body weight (bw) derived for Δ9-THC by the European Food Safety Authority (EFSA). For 7 samples, only 2 drops of oil would be sufficient to exceed the ARfD of Δ9-THC.

对 22 种 CBD 油、2 种 CBG(大麻酚)油和 2 种 CBN(大麻酚)油中的 19 种大麻素(包括 Δ9-THC(四氢大麻酚)和 CBD(大麻二酚))进行了定量分析,这些油在市场上作为食品补充剂、芳香油或化妆品制剂销售。分析采用高效液相色谱-串联质谱法(HPLC-MS/MS)进行。精油中 CBD(或 CBG、CBN)的申报浓度介于 2.5% 和 20% 之间。与申报浓度相比,实际浓度从 81% 到 226% 不等。发现每滴 CBD 的浓度高达 9 毫克。在 26 个样本中的 20 个样本中检测到了Δ9-四氢大麻酚,浓度从 5 毫克/千克到 1576 毫克/千克不等(平均值 = 536 毫克/千克)。考虑到一家生产商建议的最高日摄入量(20 滴)和 70 千克的体重,50% 的产品(n = 13)中测得的 Δ9-THC 浓度将超过欧洲食品安全局(EFSA)为 Δ9-THC 制定的急性参考剂量(ARfD),即 1 微克/千克体重。在 7 个样本中,只需 2 滴油就足以超过 Δ9-THC 的急性参考剂量。
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引用次数: 0
RNA-based crop protection: potential, challenges, and practical examples 基于 RNA 的作物保护:潜力、挑战和实例
IF 2.4 3区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2024-06-12 DOI: 10.1007/s00003-024-01507-7
Aline Koch
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引用次数: 0
Amtliche Untersuchungsergebnisse von Einweg-E-Zigaretten aus dem Jahr 2022 in Deutschland 德国 2022 年起对一次性电子烟的官方测试结果
IF 2.4 3区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-06-09 DOI: 10.1007/s00003-024-01510-y
Frauke Kirsch, Eva-Maria Kröner, R. Lange, J. Niemeyer, Miriam Laible, J. Seidel, Katrin Paulus, Annette Stephani, S. Tamosaite, K. Dünnebier
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引用次数: 0
Correction: 121. ALS-Sitzung 更正:第 121 届 ALS 会议
IF 2.4 3区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2024-05-20 DOI: 10.1007/s00003-024-01504-w
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引用次数: 0
Malawi’s ‘anything goes’ food market: weak regulations on nutrition and health claims 马拉维 "随心所欲 "的食品市场:营养和健康声明监管薄弱
IF 2.4 3区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2024-04-27 DOI: 10.1007/s00003-024-01509-5
Rutendo Bell, Aggrey Pemba Gama, Limbikani Matumba

Regulations on nutrition and health claims are useful to protect consumers from deceptive practices of unscrupulous food manufacturers. Consumer protection is best achieved when relevant regulations are available and enforced. So far, no study has analyzed the robustness of regulations on nutrition and health claims in Malawi. In the present study, Malawi’s regulation was compared with Codex Alimentarius as well as European, U.S American, and the Republic of South Africa’s (RSA) regulations on nutrition and health claims. The study revealed that Malawi does not have regulations or guidelines on health claims and therefore, any health claim can be made. Likewise, Malawi’s regulation on nutrition claims was found to be the least demanding and less elaborate. Out of 11 nutrition claims related requirements, the Malawi regulation satisfied only 3. The lack of comprehensive nutrition and health claims regulations in Malawi may give an opportunity to dishonest manufacturers to mislead and deceive consumers. False claims can also derail government’s efforts towards addressing some nutrient deficiencies through food fortification programs. Currently, it is difficult to effectively regulate nutrition and health claims in Malawi. Therefore, the findings are a wake-up call for Malawi and other countries that may be in a similar situation to take the necessary remedial actions.

有关营养和健康声明的法规有助于保护消费者免受不良食品制造商欺骗行为的侵害。有了相关法规并加以执行,才能最好地保护消费者。迄今为止,还没有研究分析过马拉维营养与健康声明法规的健全性。在本研究中,马拉维的法规与《食品法典》以及欧洲、美国和南非共和国(RSA)的营养与健康声明法规进行了比较。研究表明,马拉维没有关于健康声称的法规或准则,因此可以提出任何健康声称。同样,马拉维关于营养声称的规定也是要求最低、最不详细的。在 11 项与营养声称有关的要求中,马拉维的法规只满足了 3 项要求。马拉维缺乏全面的营养和健康声称法规可能会给不诚实的制造商提供误导和欺骗消费者的机会。虚假声明还可能破坏政府通过食品营养强化计划解决某些营养素缺乏问题的努力。目前,马拉维很难对营养和健康声明进行有效监管。因此,这些发现为马拉维和其他可能面临类似情况的国家敲响了警钟,要求它们采取必要的补救措施。
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引用次数: 0
Efficiency of working coveralls and chemical resistant gloves in reducing operator exposure to pesticides 工作服和防化手套在减少操作员接触杀虫剂方面的效率
IF 2.4 3区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2024-04-25 DOI: 10.1007/s00003-024-01506-8
C. J. Kuster, F. Kluxen, E. Felkers, Neil Morgan, Nicola J. Hewitt, Julien Durand-Réville
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引用次数: 0
SocialLab: Durch Forschung und Zusammenarbeit hin zu einer nachhaltigen landwirtschaftlichen Nutztierhaltung 社会实验室:通过研究与合作实现可持续畜牧业
IF 2.4 3区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2024-04-23 DOI: 10.1007/s00003-024-01484-x
Marie von Meyer-Höfer, M. Hartmann, Peter Kenning, J. Luy, Marcus Mergenthaler, J. Roosen, A. Spiller, I. Christoph-Schulz
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引用次数: 0
Work package 1: Monitoring of the social acceptance of livestock farming 工作包 1:监测社会对畜牧业的接受程度
IF 2.4 3区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2024-04-22 DOI: 10.1007/s00003-024-01485-w
I. Christoph-Schulz, Ivica Faletar, Monika Hartmann, Peter Kenning, Jörg Luy, Marcus Mergenthaler, Jutta Roosen, Achim Spiller, Marie von Meyer-Höfer
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引用次数: 0
Zukunft durch Beteiligung – landwirtschaftliche Tierhaltung im Dialog 参与成就未来--对话中的农业畜牧业
IF 2.4 3区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2024-04-17 DOI: 10.1007/s00003-024-01483-y
Marie von Meyer-Höfer, Philipp von Gall, Bea Bardusch, Ivica Faletar, Marcus Mergenthaler, Jörg Luy, Inken Christoph-Schulz

This article presents a future workshop on agricultural livestock farming, which—as part of the SocialLab II project—developed and applied innovative methods including participating interest groups in the political transformation of livestock farming. This future workshop examines the desirable developments for agricultural livestock farming from the perspective of all interest groups. The interests in animal welfare, environmental and climate protection were taken into account, as well as the economic and practical requirements of the companies. Challenging for dialogs in the field of transforming animal husbandry are the sheer number of different demands from social groups. On the one hand, more and more interest groups are voicing new demands, for example in the area of climate protection. On the other hand, previously homogeneous interest groups, such as those from agriculture and from animal welfare, are becoming increasingly diversified in terms of goals, strategies and values. Animals are increasingly recognized as a group affected by politics with their own demands, although their political representation is still hardly regulated. This paper includes methodological features of the future workshop on agricultural livestock farming, the identified target images for agricultural livestock farming, effective approaches to stakeholder dialog and insights into the aspects of appreciation and communication in dialog between stakeholders. From a large number of positions and submissions, 3 future scenarios of livestock farming in Germany were identified: (i) higher standards, (ii) system change and reduction and (iii) alternatives to livestock farming. In future, policy measures and dialog formats in the field of livestock farming should disclose where they fit into this spectrum of objectives. Alliances and compromises between the visions of the future are possible in various combinations. Agreement on minimum legal requirements in animal welfare seems particularly urgent, as these form the starting point of the debate. In future, dialog formats should be more open about their contribution to the debate, whereby three functional areas of dialog formats can be distinguished.

作为社会实验室 II 项目的一部分,该项目开发并应用了创新方法,让利益集团参与到畜牧业的政治变革中来。该未来研讨会从所有利益集团的角度探讨了农业畜牧业的理想发展。动物福利、环境和气候保护方面的利益,以及企业的经济和实际需求都被考虑在内。畜牧业转型领域的对话所面临的挑战是来自社会群体的大量不同需求。一方面,越来越多的利益群体提出了新的要求,例如在气候保护方面。另一方面,以往同质化的利益群体,如农业利益群体和动物福利利益群体,在目标、战略和价值观方面正变得越来越多样化。动物作为受政治影响的一个群体,有着自己的诉求,这一点正日益得到认可,尽管它们的政治代表权还很难得到规范。本文包括未来农业畜牧业研讨会的方法论特点、已确定的农业畜牧业目标形象、利益相关者对话的有效方法以及对利益相关者对话中的欣赏和沟通方面的见解。从大量的立场和意见中,确定了德国畜牧业的三种未来情景:(i) 更高的标准,(ii) 系统变革和减少,(iii) 畜牧业的替代方案。今后,畜牧业领域的政策措施和对话形式应明确其与这些目标的契合点。未来愿景之间的联盟和妥协可以通过各种组合实现。就动物福利方面的最低法律要求达成一致似乎尤为迫切,因为这构成了辩论的起点。今后,对话形式应更加开放地参与辩论,对话形式可分为三个功能领域。
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Journal of Consumer Protection and Food Safety
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