Bioethanol Manufacturing from α-Cellulose Waste of Empty Palm Oil Frugs (Elaeis guineensis jack) with Hydrolysis Concetration Variations HCl and Cellulase Enzyme

Indra Masmur, Herliana Herliana, Bramwell Sitompul, E. Sitinjak
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Abstract

This study uses raw materials containing lignocellulose, namely empty fruit bunches of oil palm. Oil palm empty fruit bunches were isolated to produce cellulose, hydrolyzed into simple sugars, fermented, and distilled. From the isolation of cellulose obtained α-cellulose of 19.9612 grams (26.6149%). Then it was hydrolyzed using HCl with a concentration variation of 15%; 20%; 25%; 30%; and enzymatically hydrolyzed using cellulase to produce simple sugars which were tested qualitatively with Benedict's reagent and Tollens reagent, then quantitatively tested by the Luff Schroll method. The higher the concentration of acid used, the higher the sugar will be. The best bioethanol obtained from acid hydrolysis is using 30% HCl with ethanol content of 6.54% and enzymatic 7.32%.
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不同水解浓度盐酸和纤维素酶对棕榈空果α-纤维素废制备生物乙醇的影响
本研究使用含有木质纤维素的原料,即油棕的空果束。油棕空果束被分离出来生产纤维素,水解成单糖,发酵和蒸馏。从纤维素中分离得到α-纤维素19.9612 g(26.6149%)。然后用浓度变化为15%的HCl水解;20%;25%;30%;用纤维素酶水解得到单糖,用Benedict试剂和Tollens试剂进行定性检测,再用Luff Schroll法进行定量检测。酸的浓度越高,糖的含量就越高。盐酸用量为30%,乙醇含量为6.54%,酶解乙醇含量为7.32%时,酸水解得到的生物乙醇效果最佳。
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