Effects of alcohol-graded concentrations on total thiols and some thiol utilizing enzymes

Bayero Journal of Pure and Applied Sciences Pub Date : 2022-12-09 DOI:10.4314/bajopas.v15i1.29

D. I. Akinloye, R. Ugbaja, A. Akamo, M. A. Toriola, A. Adewale, E. I. Ugwor, A. S. James

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Abstract

Excessive intake of alcohol has been documented to initiate different pathological conditions. Although various researchers have reported these associations, the modulatory effects on endogenous thiols are not well studied. This study investigated the effects of alcohol-graded concentrations on some thiol utilizing enzymes in rats. Adults’ male rats were divided into four main groups and treated with distilled water, 30 %, 40 % and 50 % alcohol (3.20 g / Kg body weight). Five rats from each group were sacrificed at the end of 1, 7, 14, 21, and 28 day(s) of the experiment. Assay of glutathione peroxidase and glutathione S-transferase specific activities along with total thiols levels were carried out. Alcohol administration resulted in an upregulation of the activities of glutathione peroxidase and glutathione S-transferase with concomitant depletion of total thiols concentrations. Conclusively, this study affirms that graded dosages of alcohol administration to rats induced perturbations in the thiol utilizing system in a non-time dependent consistent manner.

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醇级浓度对总硫醇和部分硫醇利用酶的影响

过量饮酒已被证明会引发不同的病理状况。尽管许多研究人员已经报道了这些关联，但内源性硫醇的调节作用尚未得到很好的研究。本研究探讨了不同浓度酒精对大鼠体内某些硫醇利用酶的影响。将成年雄性大鼠分为4个主要组，分别用蒸馏水、30%、40%和50%酒精(3.20 g / Kg体重)处理。实验1、7、14、21、28 d时，每组各处死5只大鼠。测定谷胱甘肽过氧化物酶和谷胱甘肽s -转移酶特异性活性与总硫醇水平的关系。酒精管理导致谷胱甘肽过氧化物酶和谷胱甘肽s -转移酶活性的上调，并伴随总硫醇浓度的消耗。最后，本研究证实，给大鼠分级剂量的酒精以非时间依赖的一致方式诱导巯基利用系统的扰动。

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Bayero Journal of Pure and Applied Sciences

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