Stretching of DNA molecules on mica surfaces by magnetic field

2017 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO) Pub Date : 2017-08-01 DOI:10.1109/3M-NANO.2017.8286339

Feifei Wang, Ying Wang, Tingting Huang, Fenfen Guo, Jinyun Liu, Zhengxun Song, Z. Weng, Zuobin Wang

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引用次数: 1

Abstract

This paper presents a method for the stretching of DNA molecules on mica surfaces by magnetic field. In the experiment, the magnetic fields with different intensities were generated using a cylindrical coil and were used to study their effects on DNA molecules. The magnetic field intensity was controlled by the electric current flowing through the coil. The imaging of DNA molecules was performed by atomic force microscope (AFM) in the same position on the mica surface after the treatment with the magnetic field. It was found that the DNA molecules were gradually straightened with the increase of magnetic field intensity, and the straightening performance was better than that of the molecular combing method in the experiment. In addition, the degree of DNA straightening was related to the speed at which DNA molecules were dropped to the mica surface, the amount of DNA charges and the direction of magnetic field.

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磁场对云母表面DNA分子的拉伸

本文提出了一种利用磁场对云母表面DNA分子进行拉伸的方法。在实验中，使用圆柱形线圈产生不同强度的磁场，并用于研究它们对DNA分子的影响。磁场强度由流经线圈的电流来控制。利用原子力显微镜(AFM)对经磁场处理后的云母表面相同位置的DNA分子进行成像。结果发现，随着磁场强度的增加，DNA分子逐渐被拉直，且实验中的拉直性能优于分子梳理方法。此外，DNA的矫直程度与DNA分子落向云母表面的速度、DNA的电荷量和磁场方向有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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2017 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO)

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