Regeneration of Plantlets from Rhizome Bud Explants of Lasia spinosa (Lour.) Thwaites- A Medicinal Plants of Assam

Abstract

ABASTRACT In numerable medicinal plants are commercially propagated through tissue culture for large production of elite material (Rhizome buds of Lasia spinosa {Lour.}). Thwaites could be induced on Murashige and Skoog (MS) medium supplemented with different concentrations and combinations of kinetin (kin) and 6-benzyl amino purine (BAP) alone and in combination with Naphthaleneacetic acid (NAA). Lasia is one of the traditionally important plants of Assam, which is employed in the treatment of gastrointestinal diseases, respiratory diseases and skin infections. This plant is also a rich source of dietary fibers, reported containing polyphenols, ascorbic acid and hydrocyanic acid. The present study aimed to establish producible protocol for in vitro regeneration of Lasia spinosa using rhizome bud explants. For shoot proliferation, among the various concentrations, 3.0 mgL BAP showed the highest shoot regeneration frequency of 88.2±2.8%. The highest number of shoot were recorded as 1.9 ± 0.45 in L. spinosa, but the highest shoot length (4.5±0.07 cm) was observed at reduced concentration of BAP (1.0 mgL). Plantlets rooted in 1⁄2 strength MS medium augmented with 0.1–1.0 mgL either NAA or IBA for L. spinosa for root formation. The highest percentage (79.5±2.6%), maximum number of rootlets/ shoot let (4.0±0.46) and mean length of rootlets (3.25±0.06 cm) were observed in L. spinosa. Our findings have paved a way for future investigation on another mode of regeneration like haploid production, anther culture etc and also for the commercial and rapid propagation of L. spinosa.