М.Y. Rubtsova, N. Fursova, G. Presnova, M. Ulyashova, V. Grigorenko, А. Egorov
{"title":"HYBRIDIZATION ANALYSIS ON COLORIMETRIC BIOCHIPS FOR QUANTITATIVE DETERMINATION OF BETA-LACTAMASE GENES IN ANTIBIOTIC RESISTANT BACTERIA","authors":"М.Y. Rubtsova, N. Fursova, G. Presnova, M. Ulyashova, V. Grigorenko, А. Egorov","doi":"10.37747/2312-640x-2021-19-252-254","DOIUrl":null,"url":null,"abstract":"A method of hybridization analysis on colorimetric biochips for the quantitative determination of nucleic acids has been developed. The assay conditions and the method of processing digital images of biochips using different color models have been optimized. The method was applied for the quantitative determination of DNA and mRNA of serine beta-lactamases in Gram-negative bacteria resistant to beta-lactam antibiotics.","PeriodicalId":13077,"journal":{"name":"http://eng.biomos.ru/conference/articles.htm","volume":"1 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"http://eng.biomos.ru/conference/articles.htm","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.37747/2312-640x-2021-19-252-254","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
A method of hybridization analysis on colorimetric biochips for the quantitative determination of nucleic acids has been developed. The assay conditions and the method of processing digital images of biochips using different color models have been optimized. The method was applied for the quantitative determination of DNA and mRNA of serine beta-lactamases in Gram-negative bacteria resistant to beta-lactam antibiotics.