Pub Date : 2021-01-01DOI: 10.37747/2312-640x-2021-19-96-99
E. Kudryashova
Natural (cytochrome C) and artificial electron acceptors (1,4-benzoquinone, phenazine methosulfate, tetramethylphenylenediamine) were characterized, and the possibility of using the acceptors to determine the enzymatic activity of L-galactone-1,4-lactone dehydrogenase of Arabidopsis Thaliana in an aqueous solution and in a micellar system was investigated.
{"title":"SEARCH AND CHARACTERIZATION OF ELECTRONOACEPTORS FOR L-GALACTONE-1,4-LACTONE DEHYDROGENASE FROM ARABIDOPSIS THALIANA","authors":"E. Kudryashova","doi":"10.37747/2312-640x-2021-19-96-99","DOIUrl":"https://doi.org/10.37747/2312-640x-2021-19-96-99","url":null,"abstract":"Natural (cytochrome C) and artificial electron acceptors (1,4-benzoquinone, phenazine methosulfate, tetramethylphenylenediamine) were characterized, and the possibility of using the acceptors to determine the enzymatic activity of L-galactone-1,4-lactone dehydrogenase of Arabidopsis Thaliana in an aqueous solution and in a micellar system was investigated.","PeriodicalId":13077,"journal":{"name":"http://eng.biomos.ru/conference/articles.htm","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75441401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.37747/2312-640x-2021-19-91-92
D. Ryazantsev, E. Svirshchevskaya, E. Vodovozova
Liposomal conjugate of the mutant extracellular domain of the mouse PD-1 receptor obtained to inhibit the PD-1/PD-L1 signaling pathway.
获得了小鼠PD-1受体胞外突变域的脂质体偶联物，可抑制PD-1/PD-L1信号通路。
{"title":"PREPARATION OF A LIPOSOMAL CONJUGATE OF THE MUTANT EXTRACELLULAR DOMAIN OF THE MOUSE PD-1 RECEPTOR","authors":"D. Ryazantsev, E. Svirshchevskaya, E. Vodovozova","doi":"10.37747/2312-640x-2021-19-91-92","DOIUrl":"https://doi.org/10.37747/2312-640x-2021-19-91-92","url":null,"abstract":"Liposomal conjugate of the mutant extracellular domain of the mouse PD-1 receptor obtained to inhibit the PD-1/PD-L1 signaling pathway.","PeriodicalId":13077,"journal":{"name":"http://eng.biomos.ru/conference/articles.htm","volume":"81 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73251817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.37747/2312-640x-2021-19-199-200
V. Levitsky, D. Oshchepkov, E. Zemlyanskaya
A new structural variant of the transcription factor EIN3 binding site has been obtained. This structural variant is strongly associated with the transcriptional response to ethylene and is preferred for EIN3 binding. Based on this structural variant, a new genetic ethylene sensor was developed.
{"title":"DESIGN OF A NEW ETHYLENE PLANT HORMONE SENSOR BASED ON GENOME-WIDE DATA ANALYSIS OF EIN3 TRANSCRIPTION FACTOR BINDING","authors":"V. Levitsky, D. Oshchepkov, E. Zemlyanskaya","doi":"10.37747/2312-640x-2021-19-199-200","DOIUrl":"https://doi.org/10.37747/2312-640x-2021-19-199-200","url":null,"abstract":"A new structural variant of the transcription factor EIN3 binding site has been obtained. This structural variant is strongly associated with the transcriptional response to ethylene and is preferred for EIN3 binding. Based on this structural variant, a new genetic ethylene sensor was developed.","PeriodicalId":13077,"journal":{"name":"http://eng.biomos.ru/conference/articles.htm","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76008906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.37747/2312-640x-2021-19-231-232
R. Shamagsumova
Operational and analytical characteristics of electrochemical biosensors based on acetylcholinesterase have been considered for the determination of enzyme inhibitors. As was shown, application of the mixtures of carbon nanomaterials increases sensitivity of determination over that of single component coatings. Biosensors were tested on determination of reversible inhibitors in urea and blood serum.
{"title":"ELECTROCHEMICAL ACETYLCHOLINESTERASE SENSORS BASED ON CARBON NANOMAERIALS FOR THE DETERMINATION OF DRUGS AND INSECTICIDES","authors":"R. Shamagsumova","doi":"10.37747/2312-640x-2021-19-231-232","DOIUrl":"https://doi.org/10.37747/2312-640x-2021-19-231-232","url":null,"abstract":"Operational and analytical characteristics of electrochemical biosensors based on acetylcholinesterase have been considered for the determination of enzyme inhibitors. As was shown, application of the mixtures of carbon nanomaterials increases sensitivity of determination over that of single component coatings. Biosensors were tested on determination of reversible inhibitors in urea and blood serum.","PeriodicalId":13077,"journal":{"name":"http://eng.biomos.ru/conference/articles.htm","volume":"99 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76116807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.37747/2312-640x-2021-19-117-118
M. Khrenova, A. V. Krivitskaya
Computational methods of molecular modeling are used to simulate the reaction mechanism of hydrolysis of N-acetyl-L-aspartyl-L-glutamate catalyzed by glutamate carboxypeptidase II to N-acetylaspartate and glutamate.
{"title":"MODELING REACTION MECHANISM OF GLUTAMATE CARBOXYPEPTIDASE – A MEMBER OF THE POLYENZYME CHAIN OF N-ACETYLASPARTATE","authors":"M. Khrenova, A. V. Krivitskaya","doi":"10.37747/2312-640x-2021-19-117-118","DOIUrl":"https://doi.org/10.37747/2312-640x-2021-19-117-118","url":null,"abstract":"Computational methods of molecular modeling are used to simulate the reaction mechanism of hydrolysis of N-acetyl-L-aspartyl-L-glutamate catalyzed by glutamate carboxypeptidase II to N-acetylaspartate and glutamate.","PeriodicalId":13077,"journal":{"name":"http://eng.biomos.ru/conference/articles.htm","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79361922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.37747/2312-640x-2021-19-59-61
R. Kamyshnsky, E. Varfolomeeva, S. Landa, Y. Kil, E. Komarova, E. Putevich, A. Konevega, T. Shtam
The efficiency of delivery of exogenous functional biomolecules to human cells using plant-derived vesicles has been demonstrated in vitro. Also the distribution of loaded plant vesicles in the tissues was studied in vivo.
{"title":"PLANT-DERIVED VESICLES AS CARRIES OF EXOGENOUS FUNCTIONAL BIOMOLECULES","authors":"R. Kamyshnsky, E. Varfolomeeva, S. Landa, Y. Kil, E. Komarova, E. Putevich, A. Konevega, T. Shtam","doi":"10.37747/2312-640x-2021-19-59-61","DOIUrl":"https://doi.org/10.37747/2312-640x-2021-19-59-61","url":null,"abstract":"The efficiency of delivery of exogenous functional biomolecules to human cells using plant-derived vesicles has been demonstrated in vitro. Also the distribution of loaded plant vesicles in the tissues was studied in vivo.","PeriodicalId":13077,"journal":{"name":"http://eng.biomos.ru/conference/articles.htm","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81499858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.37747/2312-640x-2021-19-371-373
V. A. Alferov, K. Osina, A. Boronin
The leading forms of recruiting the gifted youth to solution of innovative tasks are scientific workshops and conferences. The Russian Young Scientist School-Conference “Ecotoxicology” has been held in Tula State University since 2006, and it plays an important role in training biotechnologists in environmental field of research.
{"title":"RUSSIAN SCHOOL-CONFERENCE \"ECOTOXICOLOGY\" - A KEY ELEMENT FOR ATTRACTING STUDENTS TO SOLVE INNOVATIVE TASKS OF ENVIRONMENTAL PROTECTION BIOTECHNOLOGY","authors":"V. A. Alferov, K. Osina, A. Boronin","doi":"10.37747/2312-640x-2021-19-371-373","DOIUrl":"https://doi.org/10.37747/2312-640x-2021-19-371-373","url":null,"abstract":"The leading forms of recruiting the gifted youth to solution of innovative tasks are scientific workshops and conferences. The Russian Young Scientist School-Conference “Ecotoxicology” has been held in Tula State University since 2006, and it plays an important role in training biotechnologists in environmental field of research.","PeriodicalId":13077,"journal":{"name":"http://eng.biomos.ru/conference/articles.htm","volume":"487 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84409811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.37747/2312-640x-2021-19-106-108
N. L. Eremeev, N. G. Balabushevich, D. Volodkin, N. Klyachko
A procedure has been developed for the synthesis of cross-linking aggregates of catalase by treatment with glutaraldehyde incorporated into the pores of the vaterite matrix and subsequent dissolution of the inorganic core. The resulting aggregates have a spherical shape with a narrow particle size distribution and a high specific activity of the enzyme.
{"title":"PREPARATION OF CATALASE CROSS-LINKED ENZYME AGGREGATES ON THE BASE OF VATERITE MATRIX","authors":"N. L. Eremeev, N. G. Balabushevich, D. Volodkin, N. Klyachko","doi":"10.37747/2312-640x-2021-19-106-108","DOIUrl":"https://doi.org/10.37747/2312-640x-2021-19-106-108","url":null,"abstract":"A procedure has been developed for the synthesis of cross-linking aggregates of catalase by treatment with glutaraldehyde incorporated into the pores of the vaterite matrix and subsequent dissolution of the inorganic core. The resulting aggregates have a spherical shape with a narrow particle size distribution and a high specific activity of the enzyme.","PeriodicalId":13077,"journal":{"name":"http://eng.biomos.ru/conference/articles.htm","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85926317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.37747/2312-640x-2021-19-42-44
E. V. Bizina, T. Ermolaeva
Magnetic polymers with tetracycline molecular imprints have been synthesized. The conditions for the formation of a recognition layer of a piezoelectric sensor based on Fe3O4@MIP particles have been studied. It is shown that the use of the developed nanomaterial makes it possible to reduce the time required to prepare the sensor for analysis by 25 times in comparison with traditional methods.
{"title":"MAGNETIC MIP TETRACYCLINE NANOPARTICLES – SYNTHESIS AND APPLICATION IN PIEZOELECTRIC SENSORS","authors":"E. V. Bizina, T. Ermolaeva","doi":"10.37747/2312-640x-2021-19-42-44","DOIUrl":"https://doi.org/10.37747/2312-640x-2021-19-42-44","url":null,"abstract":"Magnetic polymers with tetracycline molecular imprints have been synthesized. The conditions for the formation of a recognition layer of a piezoelectric sensor based on Fe3O4@MIP particles have been studied. It is shown that the use of the developed nanomaterial makes it possible to reduce the time required to prepare the sensor for analysis by 25 times in comparison with traditional methods.","PeriodicalId":13077,"journal":{"name":"http://eng.biomos.ru/conference/articles.htm","volume":"131 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77092049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.37747/2312-640x-2021-19-238-240
A. Orlov, V. A. Bragina, N.V. Gyteneva, M. Nikitin, B. G. Gorshkov
Highly sensitive methods of optical and magnetic measurements have been developed and tested for analytical applications, medical diagnostics, food safety control, etc. The achieved detection limits are unique for express assays: DNA - 6 × 10-19 mol (without amplification), haptens - 16 fg/ml, protein biomarkers - 4 pg/ml.
{"title":"BIOSENSING AND MEDICAL DIAGNOSTICS BASED ON ULTRA SENSITIVE OPTICAL AND MAGNETIC MEASUREMENTS","authors":"A. Orlov, V. A. Bragina, N.V. Gyteneva, M. Nikitin, B. G. Gorshkov","doi":"10.37747/2312-640x-2021-19-238-240","DOIUrl":"https://doi.org/10.37747/2312-640x-2021-19-238-240","url":null,"abstract":"Highly sensitive methods of optical and magnetic measurements have been developed and tested for analytical applications, medical diagnostics, food safety control, etc. The achieved detection limits are unique for express assays: DNA - 6 × 10-19 mol (without amplification), haptens - 16 fg/ml, protein biomarkers - 4 pg/ml.","PeriodicalId":13077,"journal":{"name":"http://eng.biomos.ru/conference/articles.htm","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80843978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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