Increased level of Urinary 8-oxo-Gsn in Patients with Heart Failure

Abstract

Background: The level of RNA oxidation in patients with heart failure (HF) and its effect have not been well established. The aim of this study was to investigate the relationship between urinary 8-oxo-7, 8-dihydroguanosine (8-oxo-Gsn), a marker for oxidative stress to RNA and HF. Methods: A total of 191 HF patients from March 2014 to March 2018 and 155 healthy controls were included in this study, and their morning urine was collected. The level of urinary 8-oxo-Gsn was measured by ultra high-performance liquid chromatography mass spectrometry/mass spectrometry and adjusted by urinary creatinine. Heart failure with reduced ejection fraction (HFrEF) was defined as left ventricular ejection fraction (LVEF) <40%. Heart failure with preserved ejection fraction (HFpEF) was defined as LVEF ≥50% with diastolic dysfunction. Results: 8-oxo-Gsn in HF patients was significantly increased compared with age- and sexmatched healthy control subjects [4.20 (3.09–5.77) vs. 2.75 (2.59–2.85), p < 0.001]. The level of 8-oxo-Gsn was positively correlated with age (r = 0.257, p = 0.001) and N-terminal pro brain natriuretic peptide (r = 0.257, p < 0.001) and negatively correlated with diastolic blood pressure (r = −0.147, p = 0.049), hemoglobin (r = −0.181, p = 0.013), and creatinine clearance rate (r = −0.215, p = 0.004). Patients with HFrEF and HFpEF exhibited both the higher levels of 8-oxo-Gsn than control subjects (all p < 0.001) while there was no difference between HFrEF and HFpEF [4.44 (3.12–7.12) vs. 4.02 (3.10–5.67), p = 0.406]. Conclusions: The level of urinary 8-oxo-Gsn is increased in patients with HF, indicating that HF may be associated with increased oxidative damage to RNA, and urinary 8-oxoGsn be useful in the diagnosis and evaluation of HF.