Maureen G. Friedman , Shlomo Ilan , Simona Kahane , Nani Kosashvili , Yona Bir , David Lieberman
{"title":"A simple ELISA capable of distinguishing between IgG antibodies to Chlamydia trachomatis and Chlamydia pneumoniae","authors":"Maureen G. Friedman , Shlomo Ilan , Simona Kahane , Nani Kosashvili , Yona Bir , David Lieberman","doi":"10.1016/S1386-2618(97)00007-X","DOIUrl":null,"url":null,"abstract":"<div><p>Simple assays which reliably distinguish between past infection with <em>Chlamydia pneumoniae</em> and with <em>Chlamydia trachomatis</em> are needed. We developed an enzyme-linked immunosorbent assay (ELISA) for this purpose by reducing antigen cross reactive lipopolysaccharide epitopes with deoxycholate treatment and releasing antibodies of low affinity with a 6 M urea wash step. Paired serum samples from 212 patients with non-<em>C. pneumoniae</em>-associated community acquired pneumonia and single serum samples from 61 healthy students, 61 women with gynaecological complaints, and 100 blood donors were tested in the assay system. Excellent positive/negative correlation with the microimmunofluorescence (MIF) test was shown. This urea ELISA assay may be useful in assessment of past infection with these organisms, especially in epidemiological studies.</p></div>","PeriodicalId":100988,"journal":{"name":"Opportunistic Pathogens","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1997-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1386-2618(97)00007-X","citationCount":"3","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Opportunistic Pathogens","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S138626189700007X","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 3
Abstract
Simple assays which reliably distinguish between past infection with Chlamydia pneumoniae and with Chlamydia trachomatis are needed. We developed an enzyme-linked immunosorbent assay (ELISA) for this purpose by reducing antigen cross reactive lipopolysaccharide epitopes with deoxycholate treatment and releasing antibodies of low affinity with a 6 M urea wash step. Paired serum samples from 212 patients with non-C. pneumoniae-associated community acquired pneumonia and single serum samples from 61 healthy students, 61 women with gynaecological complaints, and 100 blood donors were tested in the assay system. Excellent positive/negative correlation with the microimmunofluorescence (MIF) test was shown. This urea ELISA assay may be useful in assessment of past infection with these organisms, especially in epidemiological studies.
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