首页 > 最新文献

Opportunistic Pathogens最新文献

英文 中文
Evaluation of new reagents for typing IgG to HSV-1 and HSV-2 HSV-1和HSV-2 IgG分型新试剂的评价
Pub Date : 1997-11-01 DOI: 10.1016/S1386-2618(97)00006-8
Fernando de Ory, Maria Eulalia Guisasola, Inmaculada Casas, José Manuel Echevarría

Until recently, the lack of suitable type specific assays has hampered the serological diagnosis of herpes simplex virus (HSV) infections, due to the high crossreactivity between types 1 and 2. The aim of the present paper is the evaluation of new commercial methods for the detection of HSV-1 and HSV-2-specific IgG using glycoprotein G as antigen (BioElisa HSV-1 and BioElisa HSV-2), in their application to viral diagnosis and seroepidemiological studies. Eighty two serum samples from HSV recent infections (30 samples from 13 cases), normal children (28 samples), and patients attended in clinics for sexually transmitted diseases (STD) (24 samples from 20 patients) were studied by such methods and the results compared with those obtained by a conventional indirect ELISA, and by the complement fixation test. The methods gave a type specific identification of the antibody response in nine of the 13 HSV patients. Positive results for anti-HSV-2 IgG were obtained in four cases among the STD patients but in none among the normal children. Nineteen of the former and seven of the latter were positive for anti-HSV-1. Only one sample was reactive in the HSV-1 assay, and negative by the indirect ELISA. Type specific HSV-1 and HSV-2 assays may help the serological diagnosis of HSV infections, since they allow the correct characterization of the antibody response. Bearing in mind the high specificity of the method for HSV-2 IgG, it might be useful in screening of populations for anti-HSV-2 and especially in prevention of the neonatal HSV-2 infection.

直到最近,由于1型和2型之间的高交叉反应性,缺乏合适的类型特异性检测阻碍了单纯疱疹病毒(HSV)感染的血清学诊断。本文的目的是评价以糖蛋白G为抗原检测HSV-1和HSV-2特异性IgG的新商业方法(BioElisa HSV-1和BioElisa HSV-2)在病毒诊断和血清流行病学研究中的应用。采用该方法对近期感染HSV(13例30份)、正常儿童(28份)和性传播疾病门诊就诊患者(20例24份)的82份血清样本进行了研究,并与常规间接ELISA法和补体固定试验结果进行了比较。该方法对13例HSV患者中9例的抗体反应进行了类型特异性鉴定。性病患者中抗hsv -2 IgG阳性4例,正常儿童中无阳性。前者19例,后者7例。仅有1份样品HSV-1检测阳性,间接ELISA检测阴性。类型特异性HSV-1和HSV-2检测可能有助于HSV感染的血清学诊断,因为它们允许抗体反应的正确表征。考虑到该方法对HSV-2 IgG的高特异性,它可能有助于筛选抗HSV-2人群,特别是预防新生儿HSV-2感染。
{"title":"Evaluation of new reagents for typing IgG to HSV-1 and HSV-2","authors":"Fernando de Ory,&nbsp;Maria Eulalia Guisasola,&nbsp;Inmaculada Casas,&nbsp;José Manuel Echevarría","doi":"10.1016/S1386-2618(97)00006-8","DOIUrl":"10.1016/S1386-2618(97)00006-8","url":null,"abstract":"<div><p>Until recently, the lack of suitable type specific assays has hampered the serological diagnosis of herpes simplex virus (HSV) infections, due to the high crossreactivity between types 1 and 2. The aim of the present paper is the evaluation of new commercial methods for the detection of HSV-1 and HSV-2-specific IgG using glycoprotein G as antigen (BioElisa HSV-1 and BioElisa HSV-2), in their application to viral diagnosis and seroepidemiological studies. Eighty two serum samples from HSV recent infections (30 samples from 13 cases), normal children (28 samples), and patients attended in clinics for sexually transmitted diseases (STD) (24 samples from 20 patients) were studied by such methods and the results compared with those obtained by a conventional indirect ELISA, and by the complement fixation test. The methods gave a type specific identification of the antibody response in nine of the 13 HSV patients. Positive results for anti-HSV-2 IgG were obtained in four cases among the STD patients but in none among the normal children. Nineteen of the former and seven of the latter were positive for anti-HSV-1. Only one sample was reactive in the HSV-1 assay, and negative by the indirect ELISA. Type specific HSV-1 and HSV-2 assays may help the serological diagnosis of HSV infections, since they allow the correct characterization of the antibody response. Bearing in mind the high specificity of the method for HSV-2 IgG, it might be useful in screening of populations for anti-HSV-2 and especially in prevention of the neonatal HSV-2 infection.</p></div>","PeriodicalId":100988,"journal":{"name":"Opportunistic Pathogens","volume":"9 1","pages":"Pages 39-41"},"PeriodicalIF":0.0,"publicationDate":"1997-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1386-2618(97)00006-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85174456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
A new specific serodiagnosis system for Lyme disease: use of synthetic peptides derived from outer surface protein C of Borrelia burgdorferi 一种新的莱姆病特异性血清诊断系统:利用伯氏疏螺旋体外表面蛋白C合成肽
Pub Date : 1997-11-01 DOI: 10.1016/S1386-2618(97)00003-2
M. Ikushima , S. Kawahashi , Y. Ohzeki , Y. Okuyama , E. Isogai , T. Arai , K. Matsui

A new specific serodiagnosis system for Lyme disease was developed using the highly specific partial peptide of outer surface protein C of Borrelia burgdorferi. Finally three peptides (OspC-I, -II and -III) were selected from the outer surface protein C (OspC) amino acid sequence of Borrelia burgdorferi sensu lato and were synthesized. OspC-I is located in the region that is conserved among species of Lyme disease spirochetes, whereas OspC-II and -III are located in the variable regions of the OspC from B. garinii type strain 20047. An enzyme-linked immunosorbent assay (ELISA) to detect antibodies against these synthetic peptides was carried out using sera from patients with Lyme disease. Furthermore, sera from patients with syphilis, tsutsugamushi disease and rheumatoid arthritis were used as control sera to demonstrate specificity of each peptide in the ELISA. The results showed that the false positive results in control sera of OspC-I, -II and -III ELISA for immunoglobulin M (IgM) antibody were 8, 22 and 16%, and those for IgG antibody were 11, 43 and 35%, respectively. These results suggested that the ELISA using OspC-I was the most specific. Therefore, sera from patients with Lyme disease were tested OspC-I ELISA. Of the 21 patients, 12 were in the acute phase and nine in the convalescent phase, 17 (81%) were positive by IgM or IgG ELISA. The sensitivities of IgM and IgG ELISA were 83 and 33% for acute-phase sera, and 22 and 78% for convalescent-phase sera, respectively, suggesting that the IgM response to OspC-I peptide was often detectable in the early stage of infection. Our data demonstrated that OspC-I was one of the common epitopes among species of Lyme disease spirochetes, and therefore this is a suitable antigen for serodiagnosis of early stage Lyme disease with high specificity.

利用伯氏疏螺旋体外表面蛋白C的高特异性部分肽,建立了莱姆病特异性血清诊断系统。最后从感应伯氏疏螺旋体的外表面蛋白C (OspC)氨基酸序列中选取OspC- i、-II和-III三个肽段合成。OspC- i位于莱姆病螺旋体物种之间的保守区域,而OspC- ii和-III位于加里尼贝氏杆菌型菌株20047的OspC的可变区域。利用莱姆病患者的血清,采用酶联免疫吸附试验(ELISA)检测针对这些合成肽的抗体。此外,梅毒、恙虫病和类风湿关节炎患者的血清作为对照血清,以验证ELISA中每个肽的特异性。结果表明,对照血清OspC-I、-II和-III免疫球蛋白M (IgM)抗体假阳性率分别为8%、22%和16%,IgG抗体假阳性率分别为11%、43%和35%。结果表明,OspC-I酶联免疫吸附试验特异性最强。因此,对莱姆病患者血清进行OspC-I ELISA检测。21例患者中,急性期12例,恢复期9例,ELISA检测IgM或IgG阳性17例(81%)。IgM和IgG ELISA对急性期血清的敏感性分别为83%和33%,对恢复期血清的敏感性分别为22%和78%,提示IgM对OspC-I肽的反应在感染早期往往可以检测到。结果表明,ospc - 1是莱姆病螺旋体中常见的抗原表位之一,具有较高的特异性,是早期莱姆病血清诊断的合适抗原。
{"title":"A new specific serodiagnosis system for Lyme disease: use of synthetic peptides derived from outer surface protein C of Borrelia burgdorferi","authors":"M. Ikushima ,&nbsp;S. Kawahashi ,&nbsp;Y. Ohzeki ,&nbsp;Y. Okuyama ,&nbsp;E. Isogai ,&nbsp;T. Arai ,&nbsp;K. Matsui","doi":"10.1016/S1386-2618(97)00003-2","DOIUrl":"10.1016/S1386-2618(97)00003-2","url":null,"abstract":"<div><p>A new specific serodiagnosis system for Lyme disease was developed using the highly specific partial peptide of outer surface protein C of <em>Borrelia burgdorferi</em>. Finally three peptides (OspC-I, -II and -III) were selected from the outer surface protein C (OspC) amino acid sequence of <em>Borrelia burgdorferi</em> sensu lato and were synthesized. OspC-I is located in the region that is conserved among species of Lyme disease spirochetes, whereas OspC-II and -III are located in the variable regions of the OspC from <em>B. garinii</em> type strain 20047. An enzyme-linked immunosorbent assay (ELISA) to detect antibodies against these synthetic peptides was carried out using sera from patients with Lyme disease. Furthermore, sera from patients with syphilis, tsutsugamushi disease and rheumatoid arthritis were used as control sera to demonstrate specificity of each peptide in the ELISA. The results showed that the false positive results in control sera of OspC-I, -II and -III ELISA for immunoglobulin M (IgM) antibody were 8, 22 and 16%, and those for IgG antibody were 11, 43 and 35%, respectively. These results suggested that the ELISA using OspC-I was the most specific. Therefore, sera from patients with Lyme disease were tested OspC-I ELISA. Of the 21 patients, 12 were in the acute phase and nine in the convalescent phase, 17 (81%) were positive by IgM or IgG ELISA. The sensitivities of IgM and IgG ELISA were 83 and 33% for acute-phase sera, and 22 and 78% for convalescent-phase sera, respectively, suggesting that the IgM response to OspC-I peptide was often detectable in the early stage of infection. Our data demonstrated that OspC-I was one of the common epitopes among species of Lyme disease spirochetes, and therefore this is a suitable antigen for serodiagnosis of early stage Lyme disease with high specificity.</p></div>","PeriodicalId":100988,"journal":{"name":"Opportunistic Pathogens","volume":"9 1","pages":"Pages 21-25"},"PeriodicalIF":0.0,"publicationDate":"1997-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1386-2618(97)00003-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78572227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Genotyping fluconazole resistant Candida albicans from human immunodeficiency virus positive patients 人类免疫缺陷病毒阳性患者氟康唑耐药白色念珠菌基因分型
Pub Date : 1997-11-01 DOI: 10.1016/S1386-2618(97)00004-4
Sofia Mata-Essayag , J.P. Burnie , G. Bailey , B. Mandall

Pulsed field gel electrophoresis (PFGE) after restriction with the enzyme sfiI, restriction fragment length polymorphism (RFLP) with the enzyme EcoRI, random amplification of polymorphic DNA (RAPD) with the probes 5′−3′ GCTGGTGG and GCG CAC GG and Southern blot hybridization with the species specific DNA probe 27A were used to type 19 fluconazole resistant, MIC ≥ 12.5 μg/ml, isolates of Candida albicans. These were associated with ten HIV positive patients where there had been a clinical failure on fluconazole and who were present concurrently in Monsall Hospital. Seventeen of the isolates were biotype 1. This study demonstrated that each patient was infected by a strain with a unique genotype and there was no evidence of crossinfection. Toothbrushes and, for fluconazole sensitive isolates, bed cups were heavily contaminated emphasising the need for their safe disposal. Low discrimination was produced by PFGE (2 types) based on changes in band pattern after variation in the unstable ribosomal chromosome had been excluded. Better discrimination was obtained with RFLP (10 types) RAPD (10 types) PFGE incorporating changes in band width and position (11 types) of SfiI digestion (11 types) and by Southern blot hybridization (12 types).

采用sfiI酶切后的脉冲场凝胶电泳(PFGE)、EcoRI酶切后的限制性片段长度多态性(RFLP)、5′−3′GCTGGTGG和GCG CAC GG探针随机扩增的多态性DNA (RAPD)和27A种特异性DNA探针的Southern杂交技术对19株耐氟康唑、MIC≥12.5 μg/ml的白色念珠菌进行了分型。这些与氟康唑临床治疗失败的10名HIV阳性患者有关,这些患者同时在蒙萨尔医院就诊。其中17株为生物型1。本研究表明,每位患者都感染了具有独特基因型的菌株,没有交叉感染的证据。牙刷和对氟康唑敏感的分离株床杯受到严重污染,强调需要安全处理。在排除不稳定核糖体染色体变异后,PFGE(2型)基于带型的变化产生了低鉴别。采用RFLP(10型)、RAPD(10型)结合SfiI酶切条带宽度和位置变化的PFGE(11型)和Southern blot杂交(12型)获得较好的鉴别效果。
{"title":"Genotyping fluconazole resistant Candida albicans from human immunodeficiency virus positive patients","authors":"Sofia Mata-Essayag ,&nbsp;J.P. Burnie ,&nbsp;G. Bailey ,&nbsp;B. Mandall","doi":"10.1016/S1386-2618(97)00004-4","DOIUrl":"10.1016/S1386-2618(97)00004-4","url":null,"abstract":"<div><p>Pulsed field gel electrophoresis (PFGE) after restriction with the enzyme <em>sfi</em>I, restriction fragment length polymorphism (RFLP) with the enzyme <em>Eco</em>RI, random amplification of polymorphic DNA (RAPD) with the probes 5′−3′ GCTGGTGG and GCG CAC GG and Southern blot hybridization with the species specific DNA probe 27A were used to type 19 fluconazole resistant, MIC ≥ 12.5 <em>μ</em>g/ml, isolates of <em>Candida albicans</em>. These were associated with ten HIV positive patients where there had been a clinical failure on fluconazole and who were present concurrently in Monsall Hospital. Seventeen of the isolates were biotype 1. This study demonstrated that each patient was infected by a strain with a unique genotype and there was no evidence of crossinfection. Toothbrushes and, for fluconazole sensitive isolates, bed cups were heavily contaminated emphasising the need for their safe disposal. Low discrimination was produced by PFGE (2 types) based on changes in band pattern after variation in the unstable ribosomal chromosome had been excluded. Better discrimination was obtained with RFLP (10 types) RAPD (10 types) PFGE incorporating changes in band width and position (11 types) of <em>Sfi</em>I digestion (11 types) and by Southern blot hybridization (12 types).</p></div>","PeriodicalId":100988,"journal":{"name":"Opportunistic Pathogens","volume":"9 1","pages":"Pages 27-37"},"PeriodicalIF":0.0,"publicationDate":"1997-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1386-2618(97)00004-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86163589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Value of galactomannan detection by sandwich enzyme-linked immunosorbent assay in the early diagnosis and follow-up of invasive aspergillosis 夹心酶联免疫吸附法检测半乳甘露聚糖在侵袭性曲霉病早期诊断及随访中的价值
Pub Date : 1997-11-01 DOI: 10.1016/S1386-2618(97)01087-8
Marie-Dominique Tabone , Hoang Vu-Thien , Jean-Paul Latge , Judith Landman-Parker , Patricia Perez-Castiglioni , Didier Moissenet , Guy Leverger

A new sandwich enzyme-linked immunosorbent assay (ELISA), capable of detecting 1 ng of galactomannan (GM) per ml, was prospectively evaluated for use in the diagnosis of invasive aspergillosis (IA). In the study, 723 serum samples sequentially collected from 76 immunocompromised children were analysed. The detection of GM was positive in 15 patients, and IA developed in eight of them. For seven patients, the results were considered false positives. No case of IA was observed in patients negative for GM. In this pilot study, the sensitivity, specificity, positive and negative predictive values of the test were 100%, 89%, 53% and 100% respectively. In six patients with IA, antigen detection was positive before the onset of clinical or radiological signs. Seven infected patients who cleared their antigenemia survived to aspergillosis. One patient with continuously high levels of GM died of uncontrolled infection. In spite of the possibility of false positive results, sandwich ELISA is a highly sensitive method, useful for the early diagnosis of IA and for the follow-up of infected patients.

一种新的三明治酶联免疫吸附试验(ELISA)能够检测1 ng / ml半乳甘露聚糖(GM),用于诊断侵袭性曲霉病(IA)的前瞻性评估。在这项研究中,对从76名免疫功能低下儿童中依次收集的723份血清样本进行了分析。15例患者GM检测呈阳性,其中8例发生IA。有7名患者的检测结果被认为是假阳性。在GM阴性患者中未发现IA病例。在本初步研究中,该检测的敏感性、特异性、阳性预测值和阴性预测值分别为100%、89%、53%和100%。在6例IA患者中,抗原检测在出现临床或放射学征象之前呈阳性。7名被感染的患者清除了他们的抗原性贫血,并存活到曲霉病。一名持续高水平转基因的患者死于不受控制的感染。尽管存在假阳性结果的可能性,但夹心ELISA是一种高度敏感的方法,可用于IA的早期诊断和感染患者的随访。
{"title":"Value of galactomannan detection by sandwich enzyme-linked immunosorbent assay in the early diagnosis and follow-up of invasive aspergillosis","authors":"Marie-Dominique Tabone ,&nbsp;Hoang Vu-Thien ,&nbsp;Jean-Paul Latge ,&nbsp;Judith Landman-Parker ,&nbsp;Patricia Perez-Castiglioni ,&nbsp;Didier Moissenet ,&nbsp;Guy Leverger","doi":"10.1016/S1386-2618(97)01087-8","DOIUrl":"10.1016/S1386-2618(97)01087-8","url":null,"abstract":"<div><p>A new sandwich enzyme-linked immunosorbent assay (ELISA), capable of detecting 1 ng of galactomannan (GM) per ml, was prospectively evaluated for use in the diagnosis of invasive aspergillosis (IA). In the study, 723 serum samples sequentially collected from 76 immunocompromised children were analysed. The detection of GM was positive in 15 patients, and IA developed in eight of them. For seven patients, the results were considered false positives. No case of IA was observed in patients negative for GM. In this pilot study, the sensitivity, specificity, positive and negative predictive values of the test were 100%, 89%, 53% and 100% respectively. In six patients with IA, antigen detection was positive before the onset of clinical or radiological signs. Seven infected patients who cleared their antigenemia survived to aspergillosis. One patient with continuously high levels of GM died of uncontrolled infection. In spite of the possibility of false positive results, sandwich ELISA is a highly sensitive method, useful for the early diagnosis of IA and for the follow-up of infected patients.</p></div>","PeriodicalId":100988,"journal":{"name":"Opportunistic Pathogens","volume":"9 1","pages":"Pages 7-13"},"PeriodicalIF":0.0,"publicationDate":"1997-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1386-2618(97)01087-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85788339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 14
Validity of Chlamyfast test in urethral and cervical samples for detecting Chlamydia trachomatis in comparison with direct fluorescent assay 尿道和宫颈标本衣原体试验检测沙眼衣原体与直接荧光法的有效性比较
Pub Date : 1997-11-01 DOI: 10.1016/S1386-2618(97)00002-0
A. Agaçfidan, M. Önel, S. Badur, Ö. Ang
{"title":"Validity of Chlamyfast test in urethral and cervical samples for detecting Chlamydia trachomatis in comparison with direct fluorescent assay","authors":"A. Agaçfidan,&nbsp;M. Önel,&nbsp;S. Badur,&nbsp;Ö. Ang","doi":"10.1016/S1386-2618(97)00002-0","DOIUrl":"10.1016/S1386-2618(97)00002-0","url":null,"abstract":"","PeriodicalId":100988,"journal":{"name":"Opportunistic Pathogens","volume":"9 1","pages":"Pages 57-58"},"PeriodicalIF":0.0,"publicationDate":"1997-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1386-2618(97)00002-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80503123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A simple ELISA capable of distinguishing between IgG antibodies to Chlamydia trachomatis and Chlamydia pneumoniae 一个简单的ELISA能够区分沙眼衣原体和肺炎衣原体的IgG抗体
Pub Date : 1997-11-01 DOI: 10.1016/S1386-2618(97)00007-X
Maureen G. Friedman , Shlomo Ilan , Simona Kahane , Nani Kosashvili , Yona Bir , David Lieberman

Simple assays which reliably distinguish between past infection with Chlamydia pneumoniae and with Chlamydia trachomatis are needed. We developed an enzyme-linked immunosorbent assay (ELISA) for this purpose by reducing antigen cross reactive lipopolysaccharide epitopes with deoxycholate treatment and releasing antibodies of low affinity with a 6 M urea wash step. Paired serum samples from 212 patients with non-C. pneumoniae-associated community acquired pneumonia and single serum samples from 61 healthy students, 61 women with gynaecological complaints, and 100 blood donors were tested in the assay system. Excellent positive/negative correlation with the microimmunofluorescence (MIF) test was shown. This urea ELISA assay may be useful in assessment of past infection with these organisms, especially in epidemiological studies.

需要简单的测定方法来可靠地区分过去感染的肺炎衣原体和沙眼衣原体。为此,我们开发了一种酶联免疫吸附试验(ELISA),通过脱氧胆酸处理减少抗原交叉反应性脂多糖表位,并通过6米尿素洗涤步骤释放低亲和力的抗体。对212例非丙型肝炎患者的血清样本进行配对。对61名健康学生、61名妇科疾患妇女和100名献血者的肺炎相关社区获得性肺炎和单血清样本进行检测。与微免疫荧光(MIF)试验呈极好的正/负相关。尿素酶联免疫吸附试验可用于评估过去感染这些微生物,特别是在流行病学研究中。
{"title":"A simple ELISA capable of distinguishing between IgG antibodies to Chlamydia trachomatis and Chlamydia pneumoniae","authors":"Maureen G. Friedman ,&nbsp;Shlomo Ilan ,&nbsp;Simona Kahane ,&nbsp;Nani Kosashvili ,&nbsp;Yona Bir ,&nbsp;David Lieberman","doi":"10.1016/S1386-2618(97)00007-X","DOIUrl":"10.1016/S1386-2618(97)00007-X","url":null,"abstract":"<div><p>Simple assays which reliably distinguish between past infection with <em>Chlamydia pneumoniae</em> and with <em>Chlamydia trachomatis</em> are needed. We developed an enzyme-linked immunosorbent assay (ELISA) for this purpose by reducing antigen cross reactive lipopolysaccharide epitopes with deoxycholate treatment and releasing antibodies of low affinity with a 6 M urea wash step. Paired serum samples from 212 patients with non-<em>C. pneumoniae</em>-associated community acquired pneumonia and single serum samples from 61 healthy students, 61 women with gynaecological complaints, and 100 blood donors were tested in the assay system. Excellent positive/negative correlation with the microimmunofluorescence (MIF) test was shown. This urea ELISA assay may be useful in assessment of past infection with these organisms, especially in epidemiological studies.</p></div>","PeriodicalId":100988,"journal":{"name":"Opportunistic Pathogens","volume":"9 1","pages":"Pages 43-49"},"PeriodicalIF":0.0,"publicationDate":"1997-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1386-2618(97)00007-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75588357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Rapid differentiation of Microsporum dermatophytes based on arbitrarily primed PCR amplification 基于任意引物PCR扩增的皮肤小孢子菌快速分化
Pub Date : 1997-11-01 DOI: 10.1016/S1386-2618(97)01086-6
D. Liu, S. Coloe, R. Baird, J. Pedersen
{"title":"Rapid differentiation of Microsporum dermatophytes based on arbitrarily primed PCR amplification","authors":"D. Liu, S. Coloe, R. Baird, J. Pedersen","doi":"10.1016/S1386-2618(97)01086-6","DOIUrl":"https://doi.org/10.1016/S1386-2618(97)01086-6","url":null,"abstract":"","PeriodicalId":100988,"journal":{"name":"Opportunistic Pathogens","volume":"108 1","pages":"3-6"},"PeriodicalIF":0.0,"publicationDate":"1997-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89943116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
Application of polymerase chain reaction to the detection of cytomegalovirus in bronchoalveolar lavage from lung transplant recipients 聚合酶链反应在肺移植受者支气管肺泡灌洗液巨细胞病毒检测中的应用
Pub Date : 1997-11-01 DOI: 10.1016/S1386-2618(97)01088-X
T. Bourlet , J.J. Chomel , F. Gharabaghi , M. Aymard

The use of polymerase chain reaction (PCR) for the detection of cytomegalovirus (CMV) in bronchoalveolar lavages (BAL) of immunocompromised patients was evaluated. Firstly, PCR was compared to conventional cell culture (CCC) and shell vial assay (SVA) in 65 BAL received in our laboratory on a routine basis. Sensitivity and specificity of the PCR were 100% and 87.2% respectively. The PCR method was then applied to the monitoring of 55 BAL from nine lung transplant recipients. The data illustrate the excellent sensitivity of PCR. However the predictive value of the test for the occurrence of a symptomatic infection was weak.

应用聚合酶链反应(PCR)检测免疫功能低下患者支气管肺泡灌洗液(BAL)中巨细胞病毒(CMV)。首先,对我们实验室常规接收的65例BAL进行PCR与常规细胞培养(CCC)和壳瓶法(SVA)的比较。PCR的灵敏度为100%,特异性为87.2%。将PCR方法应用于9例肺移植受者55例BAL的监测。这些数据说明PCR具有良好的灵敏度。然而,该试验对出现症状性感染的预测价值较弱。
{"title":"Application of polymerase chain reaction to the detection of cytomegalovirus in bronchoalveolar lavage from lung transplant recipients","authors":"T. Bourlet ,&nbsp;J.J. Chomel ,&nbsp;F. Gharabaghi ,&nbsp;M. Aymard","doi":"10.1016/S1386-2618(97)01088-X","DOIUrl":"10.1016/S1386-2618(97)01088-X","url":null,"abstract":"<div><p>The use of polymerase chain reaction (PCR) for the detection of cytomegalovirus (CMV) in bronchoalveolar lavages (BAL) of immunocompromised patients was evaluated. Firstly, PCR was compared to conventional cell culture (CCC) and shell vial assay (SVA) in 65 BAL received in our laboratory on a routine basis. Sensitivity and specificity of the PCR were 100% and 87.2% respectively. The PCR method was then applied to the monitoring of 55 BAL from nine lung transplant recipients. The data illustrate the excellent sensitivity of PCR. However the predictive value of the test for the occurrence of a symptomatic infection was weak.</p></div>","PeriodicalId":100988,"journal":{"name":"Opportunistic Pathogens","volume":"9 1","pages":"Pages 15-19"},"PeriodicalIF":0.0,"publicationDate":"1997-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1386-2618(97)01088-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76230665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Rapid differentiation of Microsporum dermatophytes based on arbitrarily primed PCR amplification 基于任意引物PCR扩增的皮肤微孢子菌快速分化
Pub Date : 1997-11-01 DOI: 10.1016/S1386-2618(97)01086-6
D. Liu, S. Coloe, R. Baird, J. Pedersen

Several Microsporum species are common and important human pathogens capable of causing dermatophytosis (also known as tinea or ringworm). The current laboratory procedures involving microscopic examination and in vitro culture are either time-consuming or lacking specificity. In this report, we examined five Microsporum species (i.e., M. canis, M. audouinii, M. cookei, M. gypseum and M. nanum) together with a number of Trichophyton and Epidermophyton dermatophytes in arbitrarily primed PCR. The results obtained indicated that these five Microsporum species formed characteristic DNA band patterns, which were different from each other and also from other dermatophytes such as Trichophyton and Epidermophyton using the random primer 5′-ACGGGCCAGT-3′ in the AP-PCR, allowing their rapid differentiation. In addition, although T. rubrum and T. soudanense had similar bands and were not distinguishable by this primer, other Trichophyton species such as T. mentagrophytes, T. tonsurans, T. verrucosum and T. violaceum displayed distinct band patterns in the AP-PCR. Therefore, this primer is potentially useful for determination of Trichophyton dermatophytes as well.

几种微孢子虫是常见且重要的人类病原体,能够引起皮肤癣菌病(也称为癣或癣)。目前涉及显微镜检查和体外培养的实验室程序要么耗时,要么缺乏特异性。在本报告中,我们在任意引物的PCR中检测了五种微孢子菌(即犬分枝杆菌、奥氏分枝杆菌、库克分枝杆菌、埃及分枝杆菌和南分枝杆菌)以及一些毛癣菌和表皮癣菌。结果表明,利用AP-PCR中的随机引物5′-ACGGCCAGT-3′,这五种微孢子菌形成了特征性的DNA带型,这些带型彼此不同,也不同于其他皮肤癣菌,如毛癣菌和表皮癣菌,使它们能够快速分化。此外,尽管红色毛癣菌和苏丹塞毛癣菌具有相似的条带,并且不能通过该引物进行区分,但其他毛癣菌物种,如须发毛癣菌、通脊毛癣菌(T.tonsurans)、疣状毛癣菌以及紫外毛癣菌在AP-PCR中显示出不同的条带模式。因此,该引物也有可能用于毛癣菌皮癣菌的测定。
{"title":"Rapid differentiation of Microsporum dermatophytes based on arbitrarily primed PCR amplification","authors":"D. Liu,&nbsp;S. Coloe,&nbsp;R. Baird,&nbsp;J. Pedersen","doi":"10.1016/S1386-2618(97)01086-6","DOIUrl":"https://doi.org/10.1016/S1386-2618(97)01086-6","url":null,"abstract":"<div><p>Several <em>Microsporum</em> species are common and important human pathogens capable of causing dermatophytosis (also known as tinea or ringworm). The current laboratory procedures involving microscopic examination and in vitro culture are either time-consuming or lacking specificity. In this report, we examined five <em>Microsporum</em> species (i.e., <em>M. canis, M. audouinii, M. cookei, M. gypseum</em> and <em>M. nanum</em>) together with a number of <em>Trichophyton</em> and <em>Epidermophyton</em> dermatophytes in arbitrarily primed PCR. The results obtained indicated that these five <em>Microsporum</em> species formed characteristic DNA band patterns, which were different from each other and also from other dermatophytes such as <em>Trichophyton</em> and <em>Epidermophyton</em> using the random primer 5′-ACGGGCCAGT-3′ in the AP-PCR, allowing their rapid differentiation. In addition, although <em>T. rubrum</em> and <em>T. soudanense</em> had similar bands and were not distinguishable by this primer, other <em>Trichophyton</em> species such as <em>T. mentagrophytes, T. tonsurans, T. verrucosum</em> and <em>T. violaceum</em> displayed distinct band patterns in the AP-PCR. Therefore, this primer is potentially useful for determination of <em>Trichophyton</em> dermatophytes as well.</p></div>","PeriodicalId":100988,"journal":{"name":"Opportunistic Pathogens","volume":"9 1","pages":"Pages 3-6"},"PeriodicalIF":0.0,"publicationDate":"1997-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1386-2618(97)01086-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72281704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
Use of Roche Amplicor and multiplex PCR for diagnosis of human mycobacterial infections 罗氏扩增和多重PCR在人分枝杆菌感染诊断中的应用
Pub Date : 1997-11-01 DOI: 10.1016/S1386-2618(97)00008-1
D. Liu, S. Lloyd Jones, R. Baird, J. Pedersen

The ability to determine various mycobacterial species rapidly and precisely is important for the control and prevention of mycobacterial infections in the human population. In the present study, we evaluated the Roche Amplicor Mycobacterium tuberculosis test and the Amplicor M. avium/M. intracellulare test, as well as a multiplex PCR, for diagnosis of mycobacterial infections using 86 clinical (sputum and bronchial washing) and culture specimens from 50 human patients. The Roche Amplicor M. tuberculosis test and the Amplicor M. avium/M. intracellulare test appeared to be highly sensitive and specific for direct detection of M. tuberculosis and M. avium/M. intracellulare, respectively, from clinical specimens. The use of culture derivatives was not necessary. Nonetheless, the Roche Amplicor tests had limited value in identifying other mycobacterial species as these mycobacteria invariably displayed negative reactions in the assays. The multiplex PCR, on the other hand, was capable of differentiating M. tuberculosis, M. avium and M. intracellulare from other mycobacterial species using culture specimens at relatively low cost. However, the multiplex PCR generated inconsistent results when clinical samples were used. Only 42 (48.8%) of the 86 sputum and bronchial washing specimens showed clear, specific electrophoretic bands in the multiplex PCR. Future optimisation of a hybridisation-based detection system is essential for enhanced identification of mycobacteria directly from clinical specimens by the multiplex PCR.

快速准确地确定各种分枝杆菌种类的能力对于控制和预防人群中的分枝杆菌感染非常重要。在本研究中,我们评估了Roche Amplicor结核分枝杆菌试验和Amplicor M. avium/M。利用来自50名人类患者的86份临床(痰液和支气管洗涤)和培养标本进行细胞内试验和多重PCR诊断分枝杆菌感染。罗氏扩增结核分枝杆菌试验和扩增结核分枝杆菌/M。细胞内试验对直接检测结核分枝杆菌和鸟分枝杆菌具有高度的敏感性和特异性。细胞内,分别来自临床标本。没有必要使用文化衍生品。尽管如此,罗氏扩增试验在鉴定其他分枝杆菌种类方面的价值有限,因为这些分枝杆菌在检测中总是显示阴性反应。另一方面,多重PCR能够以相对较低的成本利用培养标本将结核分枝杆菌、鸟分枝杆菌和胞内分枝杆菌与其他分枝杆菌种类区分开来。然而,当使用临床样本时,多重PCR产生的结果不一致。86例痰和支气管洗涤标本中,仅有42例(48.8%)在多重PCR中显示清晰、特异的电泳条带。未来基于杂交的检测系统的优化对于通过多重PCR直接从临床标本中增强分枝杆菌的鉴定至关重要。
{"title":"Use of Roche Amplicor and multiplex PCR for diagnosis of human mycobacterial infections","authors":"D. Liu,&nbsp;S. Lloyd Jones,&nbsp;R. Baird,&nbsp;J. Pedersen","doi":"10.1016/S1386-2618(97)00008-1","DOIUrl":"10.1016/S1386-2618(97)00008-1","url":null,"abstract":"<div><p>The ability to determine various mycobacterial species rapidly and precisely is important for the control and prevention of mycobacterial infections in the human population. In the present study, we evaluated the Roche Amplicor <em>Mycobacterium tuberculosis</em> test and the Amplicor <em>M. avium/M. intracellulare</em> test, as well as a multiplex PCR, for diagnosis of mycobacterial infections using 86 clinical (sputum and bronchial washing) and culture specimens from 50 human patients. The Roche Amplicor <em>M. tuberculosis</em> test and the Amplicor <em>M. avium/M. intracellulare</em> test appeared to be highly sensitive and specific for direct detection of <em>M. tuberculosis</em> and <em>M. avium/M. intracellulare</em>, respectively, from clinical specimens. The use of culture derivatives was not necessary. Nonetheless, the Roche Amplicor tests had limited value in identifying other mycobacterial species as these mycobacteria invariably displayed negative reactions in the assays. The multiplex PCR, on the other hand, was capable of differentiating <em>M. tuberculosis, M. avium</em> and <em>M. intracellulare</em> from other mycobacterial species using culture specimens at relatively low cost. However, the multiplex PCR generated inconsistent results when clinical samples were used. Only 42 (48.8%) of the 86 sputum and bronchial washing specimens showed clear, specific electrophoretic bands in the multiplex PCR. Future optimisation of a hybridisation-based detection system is essential for enhanced identification of mycobacteria directly from clinical specimens by the multiplex PCR.</p></div>","PeriodicalId":100988,"journal":{"name":"Opportunistic Pathogens","volume":"9 1","pages":"Pages 51-55"},"PeriodicalIF":0.0,"publicationDate":"1997-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1386-2618(97)00008-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89112042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
期刊
Opportunistic Pathogens
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1