H. Noritomi, Jumpei Nishigami, N. Endo, Satoru Kato, S. Takagi
{"title":"Effect of Solvent on Catalysis of Protease Adsorbed on Biochar in Organic Media","authors":"H. Noritomi, Jumpei Nishigami, N. Endo, Satoru Kato, S. Takagi","doi":"10.5539/JMSR.V7N4P46","DOIUrl":null,"url":null,"abstract":"We have found that the adsorption immobilization of the serine protease α-chymotrypsin (Alpha-CT) onto bamboo charcoal powder (BCP), which is a kind of biochar, improves the transesterification rate of N-acetyl-L-tyrosine ethyl ester (N-Ac-Tyr-OEt) with n-butanol (BuOH) in 9 organic solvents. Organic solvents strongly affected the catalysis of BCP-adsorbed Alpha-CT. The transesterification rate of BCP-adsorbed Alpha-CT was much superior to that of free Alpha-CT in every organic solvent. Especially, the transesterification rate of BCP-adsorbed Alpha-CT was about 760 times higher than that of free α-CT in n-butyl acetate.","PeriodicalId":16111,"journal":{"name":"Journal of Materials Science Research","volume":"150 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2018-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"5","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Materials Science Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5539/JMSR.V7N4P46","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 5
Abstract
We have found that the adsorption immobilization of the serine protease α-chymotrypsin (Alpha-CT) onto bamboo charcoal powder (BCP), which is a kind of biochar, improves the transesterification rate of N-acetyl-L-tyrosine ethyl ester (N-Ac-Tyr-OEt) with n-butanol (BuOH) in 9 organic solvents. Organic solvents strongly affected the catalysis of BCP-adsorbed Alpha-CT. The transesterification rate of BCP-adsorbed Alpha-CT was much superior to that of free Alpha-CT in every organic solvent. Especially, the transesterification rate of BCP-adsorbed Alpha-CT was about 760 times higher than that of free α-CT in n-butyl acetate.
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