Regulation of Foxp3-mRNA expression and Th1/Th2 immune response in patients with chronic obstructive pulmonary disease

Abstract

Objective To analyze the expression of Foxp3-mRNA and the regulation of Th1/Th2 immune response in patients with chronic obstructive pulmonary disease (COPD), and to provide a theoretical basis for the monitoring of immune status and its related mechanisms in patients with COPD. Methods A group sampling method was used to select 95 cases of patients with COPD who received a follow-up treatment in a third-grade hospital in the same hospital and 85 controls were conducted at the same time, from March 2016 to December 2017.The Foxp3-mRNA fragment was amplified by PCR and the expression of Foxp3-mRNA in serum was detected by Western blot.The expression of Th1/Th2 immune response system expression assay (including IFN-γ, IL-2, IL-4, IL-10), the obtained data were entered into SPSS 16.0 for statistical analysis. Results The level of IFN-γ, IL-2, IL-4, IL-10 in COPD group were higher than those of in the control group and there were statistical differences(t=7.282, 5.996, 7.380, 4.835, all P<0.05). In the case group, patients of different ages in IFN-γ, IL-2, IL-4, IL-10, were statistically different (t=22.237, 16.943, 45.386, 31.831, all P<0.05). In the case group, patients of different disease durations in IFN-γ, IL-2, IL-4, IL-10 were statistically different (F=33.226, 30.096, 29.353, 34.300, all P<0.05). In the case group, clear bands were seen at about 592 bp (group 1 to group 5), while the control group (group 6) and the DNA marker group (group 7) had no bands.The levels of IL-2, IL-4 and IL-10 were positively correlated and the correlation coefficients were statistically significant (r=0.614, 0.493, 0.529, 0.501, all P<0.05). Conclusions The theoretical basis for the specific expression of Foxp3-mRNA in patients with COPD is established.There is a statistically positive correlation between the expression of Foxp3-mRNA and the characteristics of Th1/Th2 immune response, but considering the epidemic of COPD in China.Characteristics, this conclusion also needs to be combined with the environmental epidemiological factors of patients with COPD to further verify the relationship between Foxp3-mRNA expression characteristics and Th1/Th2 immune response characteristics at the mechanism level. Key words: Pulmonary disease, chronic obstructive; Foxp3-mRNA; Immune response