Pub Date : 2020-04-05DOI: 10.3760/CMA.J.CN131368-20190429-00659
Chuxin Huang, Xiaona Zhan, J. Su, Changxi Miao, Jiamin Yao, Yuechun Lin, Luqian Zhou
Objective Reviewing and integrating published literature at home and abroad by meta-analysis to compare the efficacy and safety of aerosolized amikacin for treatment of ventilator-associated pneumonia (VAP). Methods Comprehensive searches were performed from the PubMed, Embase, Cochrane Central Register of Controlled Trials, Web of Science, VIP, China national knowledge internet and Wanfang database to acquire randomized controlled trials (RCTs) comparing intravenous antibiotics combined with inhalation of amikacin treatment with intravenous antibiotics and the data were collected from the time of establishment of databases to November 2018.The methodological quality of the included studies were assessed following the preferred reporting items for systematic reviews and meta-analysis guidelines.Statistical analysis on the data was performed by RevMan 5.3 software. Results Totally eight RCTs were included in the meta-analysis.Pooled analysis showed that compared to the intravenous antibiotics, intravenous antibiotics combined with inhalation of amikacin treatment significantly improved the clinical cure rate (OR=2.59, 95% CI: 1.87-3.59, P<0.000 01), bacterial clearance rate (OR=2.87, 95% CI: 1.93-4.27, P<0.000 01), and weaning rate (OR=1.88, 95%CI: 1.16-3.04, P=0.01). The death rate (OR=1.39, 95%CI: 0.86-2.24, P=0.18), the happening of bronchospasm (OR=2.30, 95%CI: 1.00-5.30, P=0.05) and renal dysfunction rate (OR=0.61, 95%CI: 0.34-1.11, P=0.10) were not significantly different for intravenous antibiotics combined with inhalation of amikacin versus intravenous antibiotics. Conclusions Patients with VAP received higher rate of clinical cure rate, bacterial clearance rate, and weaning rate and by using intravenous antibiotics combined with inhalation of amikacin treatment, whereas could not reduce the rate of mortality and didn′t increase the happening of bronchospasm and renal dysfunction rate. Key words: Pneumonia, ventilator-associated; Meta-analysis; Inhalation; Amikacin; Intravenous antibiotics
{"title":"Efficacy and safety of intravenous antibiotics combined with inhalation of amikacin versus intravenous antibiotics for treatment of ventilator-associated pneumonia: a systematic review and meta-analysis","authors":"Chuxin Huang, Xiaona Zhan, J. Su, Changxi Miao, Jiamin Yao, Yuechun Lin, Luqian Zhou","doi":"10.3760/CMA.J.CN131368-20190429-00659","DOIUrl":"https://doi.org/10.3760/CMA.J.CN131368-20190429-00659","url":null,"abstract":"Objective \u0000Reviewing and integrating published literature at home and abroad by meta-analysis to compare the efficacy and safety of aerosolized amikacin for treatment of ventilator-associated pneumonia (VAP). \u0000 \u0000 \u0000Methods \u0000Comprehensive searches were performed from the PubMed, Embase, Cochrane Central Register of Controlled Trials, Web of Science, VIP, China national knowledge internet and Wanfang database to acquire randomized controlled trials (RCTs) comparing intravenous antibiotics combined with inhalation of amikacin treatment with intravenous antibiotics and the data were collected from the time of establishment of databases to November 2018.The methodological quality of the included studies were assessed following the preferred reporting items for systematic reviews and meta-analysis guidelines.Statistical analysis on the data was performed by RevMan 5.3 software. \u0000 \u0000 \u0000Results \u0000Totally eight RCTs were included in the meta-analysis.Pooled analysis showed that compared to the intravenous antibiotics, intravenous antibiotics combined with inhalation of amikacin treatment significantly improved the clinical cure rate (OR=2.59, 95% CI: 1.87-3.59, P<0.000 01), bacterial clearance rate (OR=2.87, 95% CI: 1.93-4.27, P<0.000 01), and weaning rate (OR=1.88, 95%CI: 1.16-3.04, P=0.01). The death rate (OR=1.39, 95%CI: 0.86-2.24, P=0.18), the happening of bronchospasm (OR=2.30, 95%CI: 1.00-5.30, P=0.05) and renal dysfunction rate (OR=0.61, 95%CI: 0.34-1.11, P=0.10) were not significantly different for intravenous antibiotics combined with inhalation of amikacin versus intravenous antibiotics. \u0000 \u0000 \u0000Conclusions \u0000Patients with VAP received higher rate of clinical cure rate, bacterial clearance rate, and weaning rate and by using intravenous antibiotics combined with inhalation of amikacin treatment, whereas could not reduce the rate of mortality and didn′t increase the happening of bronchospasm and renal dysfunction rate. \u0000 \u0000 \u0000Key words: \u0000Pneumonia, ventilator-associated; Meta-analysis; Inhalation; Amikacin; Intravenous antibiotics","PeriodicalId":10004,"journal":{"name":"Chinese Journal of Asthma","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84548095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-04-05DOI: 10.3760/CMA.J.CN131368-20190524-00774
Mingli Wei, N. Huang
Objective To investigate the effect of GRHL2 silencing on proliferation and apoptosis of lung cancer A549 cells. Methods Using RNA interference technology, the expression vector of GRHL2 silenced lentivirus was constructed to infect lung cancer A549 cells, the experiment was divided into the experimental group (GRHL2 silences the A549 cell line infected by lentivirus), the negative control group (the A549 cell line infected by no-load lentivirus), the blank control group (the A549 cell line without any treatment). The intensity of green fluorescent protein was observed by inverted fluorescence microscope, and the expression levels of GRHL2 mRNA and protein in lung cancer A549 cells were detected by quantitative real-time polymerase chain reaction and Western blot.Cell counting kit-8 was used to detect the effect of GRHL2 silencing on A549 cell proliferation.Annexin V-APC /PI reagents and flow analysis were used to analyze the effect of GRHL2 silencing on the apoptosis of A549 cells. Results The expression levels of GRHL2 mRNA and protein in the experimental group were significantly decreased than the negative control groupand blank control group (F=48.13, 42.57, both P<0.05), the proliferation ability of A549 cells was significantly decreased (F=65.64, P<0.05), and apoptosis was significantly increased (F=56.76, P<0.05). Conclusions Targeted silencing of GRHL2 can significantly inhibit the proliferation and promote apoptosis of lung cancer A549 cells, and GRHL2 is expected to become a new target for clinical lung cancer targeted therapy. Key words: Lentivirus infections; Cell proliferation; Apoptosis; GRHL2; A549
{"title":"Effect of silencing of GRHL2 on proliferation and apoptosis of lung cancer A549 cells","authors":"Mingli Wei, N. Huang","doi":"10.3760/CMA.J.CN131368-20190524-00774","DOIUrl":"https://doi.org/10.3760/CMA.J.CN131368-20190524-00774","url":null,"abstract":"Objective \u0000To investigate the effect of GRHL2 silencing on proliferation and apoptosis of lung cancer A549 cells. \u0000 \u0000 \u0000Methods \u0000Using RNA interference technology, the expression vector of GRHL2 silenced lentivirus was constructed to infect lung cancer A549 cells, the experiment was divided into the experimental group (GRHL2 silences the A549 cell line infected by lentivirus), the negative control group (the A549 cell line infected by no-load lentivirus), the blank control group (the A549 cell line without any treatment). The intensity of green fluorescent protein was observed by inverted fluorescence microscope, and the expression levels of GRHL2 mRNA and protein in lung cancer A549 cells were detected by quantitative real-time polymerase chain reaction and Western blot.Cell counting kit-8 was used to detect the effect of GRHL2 silencing on A549 cell proliferation.Annexin V-APC /PI reagents and flow analysis were used to analyze the effect of GRHL2 silencing on the apoptosis of A549 cells. \u0000 \u0000 \u0000Results \u0000The expression levels of GRHL2 mRNA and protein in the experimental group were significantly decreased than the negative control groupand blank control group (F=48.13, 42.57, both P<0.05), the proliferation ability of A549 cells was significantly decreased (F=65.64, P<0.05), and apoptosis was significantly increased (F=56.76, P<0.05). \u0000 \u0000 \u0000Conclusions \u0000Targeted silencing of GRHL2 can significantly inhibit the proliferation and promote apoptosis of lung cancer A549 cells, and GRHL2 is expected to become a new target for clinical lung cancer targeted therapy. \u0000 \u0000 \u0000Key words: \u0000Lentivirus infections; Cell proliferation; Apoptosis; GRHL2; A549","PeriodicalId":10004,"journal":{"name":"Chinese Journal of Asthma","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87352556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-04-05DOI: 10.3760/CMA.J.CN131368-20190528-00782
Wei-wei Shi, Fuhui Chen
Objective To explore the relationship between 9194A/G polymorphism of survivin gene and lung cancer, and whether it is related to smoking factors. Methods From March 2011 to April 2012, 133 lung cancer patients from the Second Affiliated Hospital of Harbin Medical University were selected as the lung cancer group and 150 healthy people as the control group.By the case-control study method, polymerase chain reaction-restriction fragment length polymorphism method was used for analyzing the 9194A/G polymorphism of survivin gene in peripheral blood samples.Further stratified analysis according to smoking degree. Results The genotype frequencies of 9194A/G of survivin gene for 9194AA, 9194AG, and 9194GG were 61.33%, 33.33%, and 5.33% in the control group, and 64.66%, 30.83%, and 4.51% in the lung cancer group.There was no statistical difference between the two groups (χ2=0.358, P=0.836). There was no significant difference in the composition of survivin gene 9194A/G between lung cancer group and control group under different smoking conditions (all P>0.05). Conclusions There may be no association between the 9194A/G polymorphism of survivin gene and the lung cancer.There was no significant association between the 9194A/G polymorphism of survivin gene and smoking. Key words: Lung neoplasms; Smoking; Gene polymorphism; survivin
{"title":"Polymorphisms in the survivin gene and the risk of lung cancer","authors":"Wei-wei Shi, Fuhui Chen","doi":"10.3760/CMA.J.CN131368-20190528-00782","DOIUrl":"https://doi.org/10.3760/CMA.J.CN131368-20190528-00782","url":null,"abstract":"Objective \u0000To explore the relationship between 9194A/G polymorphism of survivin gene and lung cancer, and whether it is related to smoking factors. \u0000 \u0000 \u0000Methods \u0000From March 2011 to April 2012, 133 lung cancer patients from the Second Affiliated Hospital of Harbin Medical University were selected as the lung cancer group and 150 healthy people as the control group.By the case-control study method, polymerase chain reaction-restriction fragment length polymorphism method was used for analyzing the 9194A/G polymorphism of survivin gene in peripheral blood samples.Further stratified analysis according to smoking degree. \u0000 \u0000 \u0000Results \u0000The genotype frequencies of 9194A/G of survivin gene for 9194AA, 9194AG, and 9194GG were 61.33%, 33.33%, and 5.33% in the control group, and 64.66%, 30.83%, and 4.51% in the lung cancer group.There was no statistical difference between the two groups (χ2=0.358, P=0.836). There was no significant difference in the composition of survivin gene 9194A/G between lung cancer group and control group under different smoking conditions (all P>0.05). \u0000 \u0000 \u0000Conclusions \u0000There may be no association between the 9194A/G polymorphism of survivin gene and the lung cancer.There was no significant association between the 9194A/G polymorphism of survivin gene and smoking. \u0000 \u0000 \u0000Key words: \u0000Lung neoplasms; Smoking; Gene polymorphism; survivin","PeriodicalId":10004,"journal":{"name":"Chinese Journal of Asthma","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88305167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-04-05DOI: 10.3760/CMA.J.CN131368-20190226-00253
Lei Wang, Xinxiao Wang, Zhong Chen, Bin Shen, Zongxing Ou
Objective To investigate the expression of long-chain non-coding RNA (lncRNA) linc00261 in non-small cell lung cancer (NSCLC) and its clinical significance. Methods Included in 100 patients with NSCLC were admitted to Haikou Hospital Affiliated to Xiangya School of Medicine, Central South University from February 2017 to September 2018 were enrolled in this study.Real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to detect the expression of lncRNA linc00261 in cancer tissues and adjacent tissues of patients, and to analyze the relationship between the expression of lncRNA linc00261 and clinicopathological characteristics of patients.The expression plasmid of lncRNA linc00261 was designed and synthesized and transfected into A549 cells.The expression of lncRNA linc00261 in transfected cells was detected by qRT-PCR, the proliferation of transfected cells was detected by MTT, and the apoptosis of transfected cells was detected by flow cytometry. Results The results of qRT-PCR showed that the average relative expression of lncRNA linc0026 1 in cancer tissues of 100 NSCLC patients was significantly lower than that in adjacent tissues (1.19±0.23), with statistical significance (t=7.753, P 0.05), but the relative expression of lncRNA linc00261 in cancer tissues of NSCLC patients with different tissue differentiation degree, clinical stage and lymph node metastasis had statistical significance (all P 0.05). After 48 hours, 72 hours and 96 hours of transfection, the cell proliferation of A549 cells transfected with the expression plasmid of lncRNA linc00261 was significantly lower than that of the negative control group and the blank control group (t=3.187, 5.549, both P<0.05). The results of flow cytometry showed that the apoptotic rate of A549 cells transfected with the expression plasmid of lncRNA linc00261 was significantly higher than that of the negative control group and the blank control group after 48 hours of transfection (χ2=4.175, 6.093, both P<0.05). Conclusions lncRNA linc00261 is down-regulated in cancer tissues of NSCLC patients and is related to the development of NSCLC.It may play an anti-oncogene role in NSCLC.Overexpression of lncRNA linc0026 can inhibit the proliferation and induce apoptosis of NSCLC cells, which may become a potential therapeutic target of NSCLC. Key words: Carcinoma, non-small-cell lung; Cell proliferation; Apoptosis; Long-chain non-coding RNA; linc00261
{"title":"The expression and significance of long-chain non-coding RNA linc00261 in non-small cell lung cancer","authors":"Lei Wang, Xinxiao Wang, Zhong Chen, Bin Shen, Zongxing Ou","doi":"10.3760/CMA.J.CN131368-20190226-00253","DOIUrl":"https://doi.org/10.3760/CMA.J.CN131368-20190226-00253","url":null,"abstract":"Objective \u0000To investigate the expression of long-chain non-coding RNA (lncRNA) linc00261 in non-small cell lung cancer (NSCLC) and its clinical significance. \u0000 \u0000 \u0000Methods \u0000Included in 100 patients with NSCLC were admitted to Haikou Hospital Affiliated to Xiangya School of Medicine, Central South University from February 2017 to September 2018 were enrolled in this study.Real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to detect the expression of lncRNA linc00261 in cancer tissues and adjacent tissues of patients, and to analyze the relationship between the expression of lncRNA linc00261 and clinicopathological characteristics of patients.The expression plasmid of lncRNA linc00261 was designed and synthesized and transfected into A549 cells.The expression of lncRNA linc00261 in transfected cells was detected by qRT-PCR, the proliferation of transfected cells was detected by MTT, and the apoptosis of transfected cells was detected by flow cytometry. \u0000 \u0000 \u0000Results \u0000The results of qRT-PCR showed that the average relative expression of lncRNA linc0026 1 in cancer tissues of 100 NSCLC patients was significantly lower than that in adjacent tissues (1.19±0.23), with statistical significance (t=7.753, P 0.05), but the relative expression of lncRNA linc00261 in cancer tissues of NSCLC patients with different tissue differentiation degree, clinical stage and lymph node metastasis had statistical significance (all P 0.05). After 48 hours, 72 hours and 96 hours of transfection, the cell proliferation of A549 cells transfected with the expression plasmid of lncRNA linc00261 was significantly lower than that of the negative control group and the blank control group (t=3.187, 5.549, both P<0.05). The results of flow cytometry showed that the apoptotic rate of A549 cells transfected with the expression plasmid of lncRNA linc00261 was significantly higher than that of the negative control group and the blank control group after 48 hours of transfection (χ2=4.175, 6.093, both P<0.05). \u0000 \u0000 \u0000Conclusions \u0000lncRNA linc00261 is down-regulated in cancer tissues of NSCLC patients and is related to the development of NSCLC.It may play an anti-oncogene role in NSCLC.Overexpression of lncRNA linc0026 can inhibit the proliferation and induce apoptosis of NSCLC cells, which may become a potential therapeutic target of NSCLC. \u0000 \u0000 \u0000Key words: \u0000Carcinoma, non-small-cell lung; Cell proliferation; Apoptosis; Long-chain non-coding RNA; linc00261","PeriodicalId":10004,"journal":{"name":"Chinese Journal of Asthma","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91528111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-04-05DOI: 10.3760/CMA.J.CN131368-20190530-00795
Yuanyuan Fang, Hui-guo Liu
Obstructive sleep apnea hypopnea syndrome (OSAHS) is a common respiratory disease.In recent years, a growing number of studies reported on the OSAHS associated with optic nerve disease, including primary open-angle glaucoma, non-arterial anterior ischemic optic neuropathy, papilledema, etc.This paper reviews the relationship between OSAHS and optic neuropathy and the pathogenesis, as well as the treatment.The purpose is to prompt OSAHS patients to pay more attention to eye symptoms, and to provide new ideas for respiratory and ophthalmic diagnosis and treatment. Key words: Sleep apnea, obstructive; Optic nerve diseases; Continuous positive airway pressure
{"title":"The relationship between obstructive sleep apnea and optic neuropathy","authors":"Yuanyuan Fang, Hui-guo Liu","doi":"10.3760/CMA.J.CN131368-20190530-00795","DOIUrl":"https://doi.org/10.3760/CMA.J.CN131368-20190530-00795","url":null,"abstract":"Obstructive sleep apnea hypopnea syndrome (OSAHS) is a common respiratory disease.In recent years, a growing number of studies reported on the OSAHS associated with optic nerve disease, including primary open-angle glaucoma, non-arterial anterior ischemic optic neuropathy, papilledema, etc.This paper reviews the relationship between OSAHS and optic neuropathy and the pathogenesis, as well as the treatment.The purpose is to prompt OSAHS patients to pay more attention to eye symptoms, and to provide new ideas for respiratory and ophthalmic diagnosis and treatment. \u0000 \u0000Key words: \u0000Sleep apnea, obstructive; Optic nerve diseases; Continuous positive airway pressure","PeriodicalId":10004,"journal":{"name":"Chinese Journal of Asthma","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76839195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-04-05DOI: 10.3760/CMA.J.CN131368-20190725-01055
Huiyan Wei, Qing Zhang
Objective To investigate the selective cyclooxygenase-2 (COX-2) inhibitor nimesulide (NIM) and in combination with cisplatin (DDP) on A549 cells in nude mice transplanted tumor angiogenesis and its mechanism for the treatment oflung cancer in vivo. Methods Lung cancer A549 cells and established A549 lung xenografts in nude mice model.According to the method of random number table, the subjects were divided into four groups for drug intervention: control group, NIM group, DDP group, NIM+ DDP group, each group of 8, and the general state was observed.Mice were killed by cervical dislocation on 22nd days, then obtained tumor tissues, weighed tumor, measured tumor diameter and calculated tumor inhibition rate.To clarify the influence of nimesulide alone or in combination with cisplatin in A549 lung cancer xenograft tumor neovascularization, labeled microvessel with CD31 and counted microvessel density using microvessel counts.Immunohistochemistry was used to detect the expression of endovascular endothelial growth factor in lung cancer A549 in nude mice. Results In this study, a nude mouse model of lung cancer A549 was successfully constructed.The tumor volume of NIM+ DDP group increased more slowly than the other three groups on the 9th, 13th, 17th and 21st day (all P<0.001). Nim+ DDP group had the largest tumor inhibition rate.The microvessel density of NIM+ DDP group was lower than that of the other three groups (F=27.861, P<0.001). Conclusions The selective COX-2 inhibitors could inhibit angiogenesis, suggesting that selective COX-2 inhibitors have anti-tumor capabilities and may be used as a lung cancer targeted therapy.The selective COX-2 inhibitor combined with cisplatin had a more significant inhibitory effect on the expression of neovascularization factors and the growth of transplanted tumor in nude mice with lung cancer A549 cells. Key words: Lung neoplasms; Vascular endothelial growth factors; Inhibition of cyclooxygenase-2
{"title":"Cox-2 inhibitors in combination with cisplatin for lung cancer research progress of angiogenesis","authors":"Huiyan Wei, Qing Zhang","doi":"10.3760/CMA.J.CN131368-20190725-01055","DOIUrl":"https://doi.org/10.3760/CMA.J.CN131368-20190725-01055","url":null,"abstract":"Objective \u0000To investigate the selective cyclooxygenase-2 (COX-2) inhibitor nimesulide (NIM) and in combination with cisplatin (DDP) on A549 cells in nude mice transplanted tumor angiogenesis and its mechanism for the treatment oflung cancer in vivo. \u0000 \u0000 \u0000Methods \u0000Lung cancer A549 cells and established A549 lung xenografts in nude mice model.According to the method of random number table, the subjects were divided into four groups for drug intervention: control group, NIM group, DDP group, NIM+ DDP group, each group of 8, and the general state was observed.Mice were killed by cervical dislocation on 22nd days, then obtained tumor tissues, weighed tumor, measured tumor diameter and calculated tumor inhibition rate.To clarify the influence of nimesulide alone or in combination with cisplatin in A549 lung cancer xenograft tumor neovascularization, labeled microvessel with CD31 and counted microvessel density using microvessel counts.Immunohistochemistry was used to detect the expression of endovascular endothelial growth factor in lung cancer A549 in nude mice. \u0000 \u0000 \u0000Results \u0000In this study, a nude mouse model of lung cancer A549 was successfully constructed.The tumor volume of NIM+ DDP group increased more slowly than the other three groups on the 9th, 13th, 17th and 21st day (all P<0.001). Nim+ DDP group had the largest tumor inhibition rate.The microvessel density of NIM+ DDP group was lower than that of the other three groups (F=27.861, P<0.001). \u0000 \u0000 \u0000Conclusions \u0000The selective COX-2 inhibitors could inhibit angiogenesis, suggesting that selective COX-2 inhibitors have anti-tumor capabilities and may be used as a lung cancer targeted therapy.The selective COX-2 inhibitor combined with cisplatin had a more significant inhibitory effect on the expression of neovascularization factors and the growth of transplanted tumor in nude mice with lung cancer A549 cells. \u0000 \u0000 \u0000Key words: \u0000Lung neoplasms; Vascular endothelial growth factors; Inhibition of cyclooxygenase-2","PeriodicalId":10004,"journal":{"name":"Chinese Journal of Asthma","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91216944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-04-05DOI: 10.3760/CMA.J.CN131368-20191015-01416
Ji-Zhong Yin, Bing Li
Neutrophils are the most common inflammation cells in the human body and the most abundant cells in the tumor microenvironment.Normal neutrophils are derived from bone marrow hematopoietic stem cells, which have the function of clearing pathogens and tissue repair.However, In the tumor background, the subpopulations of neutrophils can transform to Myeloid-derived suppressor cells (MDSCs), which is a kind of immature neutrophils with strong immunosuppressive effects.Circulating MDSCs inhibit the anti-tumor immunity of T lymphocytes by producing reactive oxygen species and arginase, and release neutrophil extracellular traps to promote tumor cell transport in blood.MDSCs in tumor tissues inhibit T lymphocytes function by expressing PD-L1, and can also release vascular endothelial growth factor to promote angiogenesis, release metal matrix protease to induce epithelial-mesenchymal transition, thereby aggravating tumor progression.Neutrophils play an important role in the development of tumorigenesis.This article reviews the origin of neutrophils and the mechanism by which neutrophils promote tumor progression in circulation and tumor tissues. Key words: Tumor microenvironment; Biological markers; Neutrophils; Myeloid-derived suppressor cells; Immunity inhibition
{"title":"The tumor promoting mechanism of circulating and tumor tissue neutrophils","authors":"Ji-Zhong Yin, Bing Li","doi":"10.3760/CMA.J.CN131368-20191015-01416","DOIUrl":"https://doi.org/10.3760/CMA.J.CN131368-20191015-01416","url":null,"abstract":"Neutrophils are the most common inflammation cells in the human body and the most abundant cells in the tumor microenvironment.Normal neutrophils are derived from bone marrow hematopoietic stem cells, which have the function of clearing pathogens and tissue repair.However, In the tumor background, the subpopulations of neutrophils can transform to Myeloid-derived suppressor cells (MDSCs), which is a kind of immature neutrophils with strong immunosuppressive effects.Circulating MDSCs inhibit the anti-tumor immunity of T lymphocytes by producing reactive oxygen species and arginase, and release neutrophil extracellular traps to promote tumor cell transport in blood.MDSCs in tumor tissues inhibit T lymphocytes function by expressing PD-L1, and can also release vascular endothelial growth factor to promote angiogenesis, release metal matrix protease to induce epithelial-mesenchymal transition, thereby aggravating tumor progression.Neutrophils play an important role in the development of tumorigenesis.This article reviews the origin of neutrophils and the mechanism by which neutrophils promote tumor progression in circulation and tumor tissues. \u0000 \u0000Key words: \u0000Tumor microenvironment; Biological markers; Neutrophils; Myeloid-derived suppressor cells; Immunity inhibition","PeriodicalId":10004,"journal":{"name":"Chinese Journal of Asthma","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88516844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-04-05DOI: 10.3760/CMA.J.CN131368-20190604-00827
S. Gu, Yu-neng Lin, Shang-zhi Wu, Zhanhang Huang, Xiaowen Chen, Danhong Su, Y. Deng, Ying-ying Gu, De-hui Chen
Objective To explore the clinical manifestations, treatment and prognosis of pulmonaryinvasive fungal infection in children. Methods The clinical, imaging, laboratory examination, treatment and prognosis of 93 children with pulmonary invasive fungal infection that were to be diagnosed or confirmed in the Department of Pediatrics, the First Affiliated Hospital of Guangzhou Medical University, from January 2012 to August 2018 were analyzed retrospectively. Results Among the 93 fungal patients, 64 were male and 29 were female, with an age of (55.18±5.38) months, ranging from one month to 15 years, and a median age of 32 months.The average length of stay was 25.4 days, and the median length of stay was 23 days.The clinical manifestations of all patients were nonspecific, including fever, cough, expectoration, shortness of breath and dyspnea.The pulmonary imaging features were diverse, mainly including slightly thickened / increased lung texture, spot or patchy fuzzy shadow between the textures, accompanied by pulmonary consolidation, diffuse nodular change, etc.Bronchiectasis and mediastinal lymph node were rare.Among the 93 patients, 41 cases (44.1%) were positive for fungal culture in blood / deep sputum, and 22 cases (53.6%) were positive for Candida, two kinds of fungi were cultured in some patients at the same time.72 cases(77.4%) were effective in antifungal therapy, eight cases (8.6%) were abandoned because of basic diseases, six cases (6.5%) were transferred to other hospital, senven cases (7.5%) died.Among them, 41 cases (44.1%) were treated with voriconazole alone or in combination, 38 cases (92.7%) were effective, and three cases (7.3%) died. Conclusions Children with pulmonary invasive fungal infection are more likely to occur in children with serious underlying diseases.Their clinical and imaging manifestations are nonspecific.The mortality and prognosis are closely related to host factors, environmental factors and antifungal treatment.The use of voriconazole antifungal treatment can effectively reduce the mortality of children with pulmonary fungal infection. Key words: Lung diseases, fungal; Pneumonia; Child; Clinical analysis; Treatment
{"title":"Retrospective analysis of 93 cases of invasive fungal infection in children′s lung","authors":"S. Gu, Yu-neng Lin, Shang-zhi Wu, Zhanhang Huang, Xiaowen Chen, Danhong Su, Y. Deng, Ying-ying Gu, De-hui Chen","doi":"10.3760/CMA.J.CN131368-20190604-00827","DOIUrl":"https://doi.org/10.3760/CMA.J.CN131368-20190604-00827","url":null,"abstract":"Objective \u0000To explore the clinical manifestations, treatment and prognosis of pulmonaryinvasive fungal infection in children. \u0000 \u0000 \u0000Methods \u0000The clinical, imaging, laboratory examination, treatment and prognosis of 93 children with pulmonary invasive fungal infection that were to be diagnosed or confirmed in the Department of Pediatrics, the First Affiliated Hospital of Guangzhou Medical University, from January 2012 to August 2018 were analyzed retrospectively. \u0000 \u0000 \u0000Results \u0000Among the 93 fungal patients, 64 were male and 29 were female, with an age of (55.18±5.38) months, ranging from one month to 15 years, and a median age of 32 months.The average length of stay was 25.4 days, and the median length of stay was 23 days.The clinical manifestations of all patients were nonspecific, including fever, cough, expectoration, shortness of breath and dyspnea.The pulmonary imaging features were diverse, mainly including slightly thickened / increased lung texture, spot or patchy fuzzy shadow between the textures, accompanied by pulmonary consolidation, diffuse nodular change, etc.Bronchiectasis and mediastinal lymph node were rare.Among the 93 patients, 41 cases (44.1%) were positive for fungal culture in blood / deep sputum, and 22 cases (53.6%) were positive for Candida, two kinds of fungi were cultured in some patients at the same time.72 cases(77.4%) were effective in antifungal therapy, eight cases (8.6%) were abandoned because of basic diseases, six cases (6.5%) were transferred to other hospital, senven cases (7.5%) died.Among them, 41 cases (44.1%) were treated with voriconazole alone or in combination, 38 cases (92.7%) were effective, and three cases (7.3%) died. \u0000 \u0000 \u0000Conclusions \u0000Children with pulmonary invasive fungal infection are more likely to occur in children with serious underlying diseases.Their clinical and imaging manifestations are nonspecific.The mortality and prognosis are closely related to host factors, environmental factors and antifungal treatment.The use of voriconazole antifungal treatment can effectively reduce the mortality of children with pulmonary fungal infection. \u0000 \u0000 \u0000Key words: \u0000Lung diseases, fungal; Pneumonia; Child; Clinical analysis; Treatment","PeriodicalId":10004,"journal":{"name":"Chinese Journal of Asthma","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91335779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-04-05DOI: 10.3760/CMA.J.CN131368-20200316-01594
Jijie Chen, Huaying Cui, Chunhua Wei, jingyu zhao
Objective To investigate the possible associations of oxidative damage and serum chemokines, IFN-γ-inducible protein-10 (IP-10), Regulated on activation normal T-cell-expressed and secreted (Rantes) in lung cancer patients. Methods Blood samples were collected from 52 lung cancer patients as lung cancer group, 34 chronic bronchitis patients as the ratio of group and 20 healthy controls as healthy control group Who were admitted to the department of geriatrics of Dongying district people′s hospital from February 2018 to February 2019.The activities of serum superoxide dismutase (SOD) were measured by xanthine oxidase method, the levels of malondialdehyde (MDA) were detected by thiobarbituric acid colorimetric method (TBA) and the serum levels of IFN-γ-inducible protein-10 (IP-10), regulated on activation normal T-cell-expressed and secreted (Rantes) were measured by enzyme-linked immunosorbent assay (ELISA). Linear correlation analysis was used to analyze the correlationship. Results The activities of serum SOD were significantly lower in lung cancer group compared to the ratio of group and healthy control group (F=29.681, P<0.05). while the levels of MDA were significantly higher in lung cancer group compared to the ratio of group and healthy control group (F=61.151, P<0.05). In addition, the activities of SOD were correlated to clinical stages in patients with lung cancerⅠ~Ⅳ period, the difference was statistically significant (F=11.734, P<0.05). The levels of IP-10 and Rantes were higher in lung cancer group than the ratio of group and healthy control group (F=22.115, 13.181, P<0.05). The levels of IP-10 and Rantes were negatively correlated with the activities of SOD (r=-0.309, -0.376, P=0.017, 0.034), while positively correlated with the levels of MDA (r=0.357, P=0.021). Conclusions Strong associations were found between chemokines and oxidative stress which participated in the pathogenesis of lung cancer. Key words: Lung neoplasms; Superoxide dismutase; Malondialdehyde; IFN-γ-inducible protein-10; Regulated on activation normal T-cell-expressed and secreted
{"title":"Correlationship between oxidative damage and IP-10, Rantes in lung cancer patients","authors":"Jijie Chen, Huaying Cui, Chunhua Wei, jingyu zhao","doi":"10.3760/CMA.J.CN131368-20200316-01594","DOIUrl":"https://doi.org/10.3760/CMA.J.CN131368-20200316-01594","url":null,"abstract":"Objective \u0000To investigate the possible associations of oxidative damage and serum chemokines, IFN-γ-inducible protein-10 (IP-10), Regulated on activation normal T-cell-expressed and secreted (Rantes) in lung cancer patients. \u0000 \u0000 \u0000Methods \u0000Blood samples were collected from 52 lung cancer patients as lung cancer group, 34 chronic bronchitis patients as the ratio of group and 20 healthy controls as healthy control group Who were admitted to the department of geriatrics of Dongying district people′s hospital from February 2018 to February 2019.The activities of serum superoxide dismutase (SOD) were measured by xanthine oxidase method, the levels of malondialdehyde (MDA) were detected by thiobarbituric acid colorimetric method (TBA) and the serum levels of IFN-γ-inducible protein-10 (IP-10), regulated on activation normal T-cell-expressed and secreted (Rantes) were measured by enzyme-linked immunosorbent assay (ELISA). Linear correlation analysis was used to analyze the correlationship. \u0000 \u0000 \u0000Results \u0000The activities of serum SOD were significantly lower in lung cancer group compared to the ratio of group and healthy control group (F=29.681, P<0.05). while the levels of MDA were significantly higher in lung cancer group compared to the ratio of group and healthy control group (F=61.151, P<0.05). In addition, the activities of SOD were correlated to clinical stages in patients with lung cancerⅠ~Ⅳ period, the difference was statistically significant (F=11.734, P<0.05). The levels of IP-10 and Rantes were higher in lung cancer group than the ratio of group and healthy control group (F=22.115, 13.181, P<0.05). The levels of IP-10 and Rantes were negatively correlated with the activities of SOD (r=-0.309, -0.376, P=0.017, 0.034), while positively correlated with the levels of MDA (r=0.357, P=0.021). \u0000 \u0000 \u0000Conclusions \u0000Strong associations were found between chemokines and oxidative stress which participated in the pathogenesis of lung cancer. \u0000 \u0000 \u0000Key words: \u0000Lung neoplasms; Superoxide dismutase; Malondialdehyde; IFN-γ-inducible protein-10; Regulated on activation normal T-cell-expressed and secreted","PeriodicalId":10004,"journal":{"name":"Chinese Journal of Asthma","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77230124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-04-05DOI: 10.3760/CMA.J.CN131368-20190514-00739
Chunlei Shao, Wenjiao Jiang, Linna Jiang, H. Yue
Obstructive sleep apnea-hypopnea syndrome (OSAHS) is a disorder of recurrent hypoxia and daytime sleepiness caused by airway collapse during sleep.Obstructive sleep apnea-hypopnea syndrome is closely related to cardiovascular diseases such as hypertension, arrhythmia, coronary atherosclerotic heart disease and heart failure.OSAHS plays an important role in promoting the occurrence and development of cardiovascular diseases.Currently, OSAHS as an important risk factor of coronary atherosclerotic heart disease is gradually paid attention to.This article reviews the pathogenesis of OSAHS induced coronary heart disease, the relationship between OSAHS and coronary artery stenosis, the adverse prognosis of OSAHS combined with coronary heart disease and the effect of continuous positive airway pressure on the prognosis of these patients. Key words: Sleep apnea, obstructive; Coronary artery disease; Coronary stenosis; Continuous positive airway pressure; Pathogenesis
{"title":"Research progress of obstructive sleep apneahypopnea syndrome and coronary heart disease","authors":"Chunlei Shao, Wenjiao Jiang, Linna Jiang, H. Yue","doi":"10.3760/CMA.J.CN131368-20190514-00739","DOIUrl":"https://doi.org/10.3760/CMA.J.CN131368-20190514-00739","url":null,"abstract":"Obstructive sleep apnea-hypopnea syndrome (OSAHS) is a disorder of recurrent hypoxia and daytime sleepiness caused by airway collapse during sleep.Obstructive sleep apnea-hypopnea syndrome is closely related to cardiovascular diseases such as hypertension, arrhythmia, coronary atherosclerotic heart disease and heart failure.OSAHS plays an important role in promoting the occurrence and development of cardiovascular diseases.Currently, OSAHS as an important risk factor of coronary atherosclerotic heart disease is gradually paid attention to.This article reviews the pathogenesis of OSAHS induced coronary heart disease, the relationship between OSAHS and coronary artery stenosis, the adverse prognosis of OSAHS combined with coronary heart disease and the effect of continuous positive airway pressure on the prognosis of these patients. \u0000 \u0000Key words: \u0000Sleep apnea, obstructive; Coronary artery disease; Coronary stenosis; Continuous positive airway pressure; Pathogenesis","PeriodicalId":10004,"journal":{"name":"Chinese Journal of Asthma","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75462588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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