Analysis of Plasma Fluorescence Spectra Mechanism

Shumei Gao, Jun Lu, L. Ge
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Abstract

the UV-induced plasma fluorescence spectra are obtained by FLS920 Spectrometer. There is a stronger and wider peak from 300nm to 410nm in the plasma fluorescence spectrum. Using soft analysis, it is found that the peak is actually consisted of two Gaussion curves, or two fluorophores, which are albumin and immunoglobulin (Ig). And their rate is about 58% and 37% completed. We are thought that the Albumin fluorescence is most, but the fluorescence information of Ig is more important for medical diagnoses. Abnormity of immune system may change Ig construction, and its spectral characteristics is altered. Therefore, based on UV-induced plasma spectra, Ig spectra can be indirectly studied by mathematical analysis. KeywordsUV-fluorescence, plasma, albumin, immunoglobulin (Ig)
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等离子体荧光光谱机理分析
紫外诱导等离子体荧光光谱由FLS920光谱仪获得。等离子体荧光光谱在300nm ~ 410nm处有一个更强、更宽的峰。通过软分析发现,该峰实际上是由两条高斯曲线或两个荧光团组成的,它们是白蛋白和免疫球蛋白(Ig)。它们的完成率约为58%和37%。我们认为白蛋白荧光是最重要的,但Ig的荧光信息对医学诊断更重要。免疫系统异常可改变Ig结构,改变其光谱特征。因此,基于紫外诱导等离子体光谱,可以通过数学分析间接研究Ig光谱。关键词:紫外荧光,血浆,白蛋白,免疫球蛋白
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