Modulation of chlorpyrifos-induced genotoxicty and oxidative stress by cow urine supplementation in male wistar rats

Abstract

Due to extensive use of chemical fertilisers, pesticides and hormone injections our food is becoming poisonous and to eliminate such poisons from our body natural products can prove to be very effective. The therapeutic use of cow urine has a long history in India. And recently being considered as potent bio pesticide, the cow urine has been considered in the present study to evaluate its ameliorative potential against the oxidative stress and genotoxicity induced in the brain tissue of rat by intoxication of chlorpyrifos (CPF). Chlorpyrifos is a broad spectrum conventional organophosphate agrochemical used to control a variety of pests in agriculture and for domestic purposes. For the experiment the antioxidant potential of cow urine was measured by DPPH assay. Further in present inquisition ratswere divided into two groups, one exposed to1/8 th , 1/4 th and 1/2 of LD 50 of CPF and other group of rats were treated with cow urine consecutively for ten days prior to exposure of CPF. Brain tissue was collected after 24hrs, 48hrs and 72hrs of treatment. Symbolic elevation in %tail DNA, tail moment and MDA levels while reduction in SOD, CAT and GST activities was noted in chlorpyrifos treated groups as compared to control groups. The effect of dose was also ascertained for all parameters. On the other hand, pre-treatment with cow urine significantly abridged the effect of CPF intoxication. Thus, it appears from the study that cow urine ameliorates pesticide induced cancer insurgence in rat because of its high antioxidant levels and can be used as a viable potential substitute for anti-cancer therapy.