Comparison of ultrasound and maceration methods on antioxidant and antimicrobial efficacy of phenolic compounds extracted from Cynodon dactylon L. of Algeria

IF 1 Q4 CHEMISTRY, MULTIDISCIPLINARY Ovidius University Annals of Chemistry Pub Date : 2022-07-01 DOI:10.2478/auoc-2022-0018
M. Tlili, Chaima Benine
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Abstract

Abstract An investigation was carried out to extract the polyphenols from Cynodon dactylon L. by ultrasound-assisted extraction (UAE) and maceration assisted extraction (MAE), and to assess the antioxidant and antimicrobial activities, as well as the evaluation of the cytotoxic effect of ethanolic extracts. The yields of crude extracts were 9.40 % for the MAE extract and 12.52 % for the UAE extract. The results showed that the extract obtained by MAE contains a high level of polyphenols and flavonoids estimated by 42.14 ± 0.75 mg EAG/gE and 23.57 ± 0.78 mg EQ/gE. In contrast, the content of condensed tannins in the extract of UAE (19.34 ± 0.48 mg EC/gE) is higher. The evaluation of the antioxidant activity revealed a considerable antioxidant response, the MAE extract represents the most active extract, with an IC50 =7.52 ± 0.037 mg/mL for the DPPH test, and 15.83 ± 0.37 mg EAA/gE for the FRAP test. The results of antimicrobial activity showed that all the strains targeted have high susceptibility to the two ethanolic extracts of Cynodon dactylon L. The evaluation of cytotoxicity against RBCs was carried, the results shown the non-toxic effect of C. dactylon extracts and hence support its ethnomedicinal application.
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超声波和浸渍法对阿尔及利亚短爪蟹酚类化合物抗氧化和抗菌效果的比较
摘要采用超声辅助提取(UAE)和浸渍辅助提取(MAE)两种方法提取大爪蟹多酚,并对乙醇提取物的抗氧化、抑菌活性和细胞毒作用进行了研究。MAE粗提物得率为9.40%,UAE粗提物得率为12.52%。结果表明,提取液中多酚类和黄酮类化合物含量较高,分别为42.14±0.75 mg EAG/gE和23.57±0.78 mg EQ/gE。UAE提取物中缩合单宁含量较高(19.34±0.48 mg EC/gE)。抗氧化活性评价显示,MAE提取物具有较强的抗氧化作用,其抗氧化活性最高,DPPH试验IC50 =7.52±0.037 mg/mL, FRAP试验IC50 = 15.83±0.37 mg EAA/gE。抑菌活性结果表明,所有目标菌株对两种短爪草乙醇提取物均有较高的敏感性,并进行了对红细胞的细胞毒性评价,结果表明短爪草提取物具有无毒作用,支持其民族医药应用。
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来源期刊
Ovidius University Annals of Chemistry
Ovidius University Annals of Chemistry CHEMISTRY, MULTIDISCIPLINARY-
自引率
11.10%
发文量
20
审稿时长
5 weeks
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