UV-Spectrophotometric Estimation and Forced Degradation Studies of Tenofovir Alafenamide Fumarate (TAF) in its Bulk and Tablet Dosage Form

Abstract

A simple, sensitive and reproducible Spectrophotometric method is developed for estimation of Tenofovir Alafenamide Fumarate (TAF) in bulk and its tablet dosage form. Forced degradation studies are also conducted on standard TAF and % degradation of drug under various stress conditions is reported. Different concentrations of TAF are prepared using distilled water: acetonitrile as diluent. The absorption maximum of TAF is found to be at 261nm. Linearity was established in the range of 6.25-37.5 μg/ml with regression coefficient of 0.999. The drug was subjected to acid and alkali hydrolysis, oxidation degradation, thermal and photo degradation conditions. © 2020 iGlobal Research and Publishing Foundation. All rights reserved. Cite this article as: Bhavyasri, K.; Manisha, M.; Rambabu, D. UV-spectrophotometric estimation and forced degradation studies of tenofovir alafenamide fumarate (TAF) in its bulk and tablet dosage form. Indo Global J. Pharm. Sci., 2020; 10(4): 1-5. DOI: http://doi.org/10.35652/IGJPS.2020.10401 . Indo Global Journal of Pharmaceutical Sciences, 2020; 10(4): 1-5 2 Simultaneous estimation of TAF and Emtricitabine by UV spectroscopic method is found in a literature. The method involving determination of TAF and Emtricitabine at 260nm and 280nm over the concentration ranges of 5-30μg/ml for both drugs has been described [6]. Most of the reported methods are often time consuming, expensive, use multi or expensive reagents, cumbersome and required expertise operational personnel. UV spectrophotometry, because of simplicity, reproducibility and speed and also it requires minimum solvent/reagent system and less analysis time, is widely used for the assay of the therapeutic compounds used as medications. MATERIALS AND METHODS Apparatus The Spectrophotometric measurements were carried out using Elico SL 210 UV/Visible spectrophotometer.