Multiplex detection of IgG class antibodies to Toxoplasma gondii, Rubella virus, Cytomegalovirus and Herpes virus using a novel multiplex flow immunoassay

S. Dolgushin, S. Selishchev, E. Loseva, E. Odintsova, A. Tronin
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引用次数: 1

Abstract

Preclinical tests of a novel multiplex immunoassay for the detection of TORCH infections in human serum using flow cytometry are presented. The immunoassay is intended for the qualitative detection of specific human IgG class antibodies to Toxoplasma gondii, Rubella virus Cytomegalovirus and Herpes virus in human serum. Multiplex analysis is performed in vitro on polystyrene microspheres encoded with organic dye. The diagnostic sensitivity of the kit is evaluated to be not less than 95%.
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用一种新的多重流免疫分析法检测刚地弓形虫、风疹病毒、巨细胞病毒和疱疹病毒IgG类抗体
一种新型的多重免疫分析法用于检测TORCH感染在人血清中使用流式细胞术的临床前试验。本方法适用于人血清中弓形虫、风疹病毒、巨细胞病毒和疱疹病毒特异性IgG类抗体的定性检测。多重分析进行了体外与有机染料编码聚苯乙烯微球。经评估,试剂盒的诊断灵敏度不低于95%。
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