Studies on inhibition activity of chitosan against bacteria from drinking water

Q. Cheng, Wen-lin Wang
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Abstract

The effects of the inhibition activity of chitosan on the bacteria isolated from drinking water were studied. The bacteriostasis circle sizes of chitosan were observed, and the inhibitory effects with different concentration of chitosan (0.1, 0.2, 0.5 mg/l) and different cell concentration were also studied respectively. The results showed that chitosan could inhibit the growth of the bacteria isolated, and the ability was directly proportional to its concentrations. Though the bactericidal effect of chitosan with 0.2 mg/l concentration was after that of 0.5 mg/l, and longer settling time was needed, the turbidity was lower for the full flocculation of the residual bacteria. 0.2 mg/l concentration of chitosan could completely inhibit the growth of bacillus with low concentration; the holding time was extended with higher concentration of bacterial suspension and the same effect was also achieved. The actual use of chitosan as a disinfectant agent should be based on the bacterial contamination of water; tests were needed to find the optimal concentration of chitosan; and the flocculation time should be adjusted to achieve satisfactory results.
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壳聚糖对饮用水细菌抑制活性的研究
研究了壳聚糖对饮用水中分离细菌的抑制作用。观察了壳聚糖的抑菌圈大小,并分别研究了不同浓度的壳聚糖(0.1、0.2、0.5 mg/l)和不同细胞浓度的抑菌效果。结果表明,壳聚糖对分离细菌的生长具有抑制作用,且抑制作用与其浓度成正比。虽然0.2 mg/l浓度的壳聚糖的杀菌效果优于0.5 mg/l浓度,且沉淀时间较长,但其浊度较低,可使残留菌充分絮凝。0.2 mg/l浓度的壳聚糖能完全抑制低浓度芽孢杆菌的生长;菌悬液浓度越高,保温时间越长,效果也越好。壳聚糖作为消毒液的实际使用应根据水的细菌污染情况而定;需要通过试验来确定壳聚糖的最佳浓度;并对絮凝时间进行调整,以达到满意的效果。
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