Existence and Virulence Designation of Listeria Monocytogenes in Retail Chilled Pork Byproducts in Cairo Porcine Markets with Trials of Using Lactobacillus Probiotic as Anti-Listerial Meat Perservative

Abstract

Background: It is well known that Listeria monocytogenes is a substantial foodborne pathogen that can boost in cooling temperature and causes serious human infections. Materials and methods: The study investigated 60 chilled pork byproducts purchased from Egyptian markets and PCR assayed for the presence of certain virulence genes answerable for the pathogenicity, besides the antilisterial activity inquiry of probiotic bacteria. Results: eleven Listeria monocytogenes isolates (18.3%) were obtained and the survey exposed that 6 isolates harbored hly gene, 7 carried inlA gene, and 7 loaded prfA gene in incidence of 54.5, 63.6, and 63.6% respectively in which the three genes were identified in three isolates (27.3%). The isolates were sensitive to Lactobacillus acidophilus more than Lactobacillus plantarum even diluted to10 -5 . Conclusion: The study revealed there were a considerable isolation percent of Listeria monocytogenes with their virulence genes in Egyptian pork byproducts. Probiotic bacteria can combat the microbe as modern and safe protection.