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Remediaton of pesticide-polluted River using Anabaena and Nostoc spp. 利用水蚤和褐藻修复农药污染河流。
Pub Date : 2017-07-01 DOI: 10.9790/2402-1107017176
J. Williams, V. Amaechi
Cyanobacteria are phototrophic aquatic microbes that are found in various environments and can reproduce rapidly either heterotrophically or chemoheterotrophically. Pesticide-pollutants are released into water bodies as a method of disposal. Bioremediation is considered an efficient and environmentally safe technology for inexpensive decontamination of such environments. Cyanobacteria species investigated in this study were highly beneficial in the remediation of the pesticide-polluted River. The Cyanobacteria isolated were identified as Anabaena and Nostoc species using Abattoir effluent and Aquaculture water from Ogoniland. Temperature, pH, phosphate and nitrate enhanced the growth of these organisms in the polluted environments with varying concentrations of pesticide {Nostoc(N14%, N240%, N380%), Anabaena (A14%, A240%, A380%), Anabaena and Nostoc (AN14%, AN240%, AN380%). The microbiological analysis showed that 80% mixed culture (AN3) had higher microbial counts especially on day 7, which could be due to the presence of a mixed consortium of organisms and nutrients (Nitrogen and Phosphorus). The effectiveness of the Cyanobacteria used in the analysis was in the following descending order: 80%AN3> 40%AN2> 4%AN1> 80%N3> 80%A3> 40%N2> 40%A2> 4%N1> 4%A1. From the student T test table, AN3 (80% mixed culture) was more effective and capable of remediating the pesticide-polluted environment. The analysis of variance (ANOVA) showed that at 95% confident level the significant differences among the samples varied in their ability to remediate the environment. Pollutant was removed by all the species, either as individuals or in a mixed consortium at all concentrations. Results established high removal efficiencies of the investigated species for the removal of the target contaminant which were species and contaminant-dependent. The contaminants removal efficiency (RE) percentage of cyanobacterial species ranged between 76.3 and 100% for 4% pesticide concentration, 83.9% and 99.9% for 40% concentration. Mixed culture ( Anabaena and Nostoc (AN)) RE percentages ranged between 99.5 and 100% at 4% while at 40%, the RE percentage ranged between 95.9 and 99.9% and at 80% pesticide concentration, it ranged between 91.7 and 100%. Results obtained stipulate the potential of natural resources as proficient mediators for pollution control.
蓝藻是在各种环境中发现的光养水生微生物,可以快速繁殖,无论是异养还是化学异养。作为一种处理方法,农药污染物被释放到水体中。生物修复被认为是对这种环境进行廉价净化的一种有效和环境安全的技术。本研究调查的蓝藻种类对农药污染河流的修复非常有益。利用Ogoniland屠宰场废水和水产养殖水分离得到的蓝藻分别为Anabaena和Nostoc两种。温度、pH、磷酸盐和硝酸盐在不同浓度农药{Nostoc(N14%、N240%、N380%)、水藻(A14%、A240%、A380%)、水藻和Nostoc(AN14%、AN240%、AN380%)的污染环境中促进了这些生物的生长。微生物学分析表明,80%混合培养(AN3)的微生物数量较高,特别是在第7天,这可能是由于微生物和营养物质(氮和磷)的混合联合体的存在。分析中使用的蓝藻菌的有效性依次为:80%AN3> 40%AN2> 4%AN1> 80%N3> 80%A3> 40%N2> 40%A2> 4%N1> 4%A1。从学生T检验表来看,AN3(80%混合培养)对农药污染环境的修复效果更好,修复能力更强。方差分析(ANOVA)显示,在95%的置信水平下,样本之间的显著差异在其修复环境的能力方面有所不同。污染物被所有物种去除,无论是作为个体还是在所有浓度的混合财团中。结果表明,所研究的菌种对目标污染物的去除率较高,且具有菌种和污染物依赖性。当农药浓度为4%时,蓝藻对污染物的去除率为76.3 ~ 100%,当农药浓度为40%时,蓝藻对污染物的去除率为83.9% ~ 99.9%。混合培养(Anabaena and Nostoc (AN))在4%和40%浓度下的回收率为99.5 ~ 100%,在95.9 ~ 99.9%之间,在80%浓度下的回收率为91.7 ~ 100%。所得结果表明,自然资源作为污染控制的熟练媒介的潜力。
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引用次数: 2
Chef’s Perception on Nutrition and Health 厨师对营养与健康的认知
Pub Date : 2017-07-01 DOI: 10.9790/2402-1107015661
D. Vandana, D. L. Kusuma
Of late there is a tremendous increase in the eating out episodes of our population. In order to meet the demand there is an increase in the establishment of Food service units the throughout the country. In the western world the consumers are habituated to eating out. In view of the increase in the incidence of obesity and associated chronic health problem such as CVD, CHD, diabetes mellitus etc. a focus is being given to healthy eating out practices. The study surveyed chef’s working in select hotels and restaurants at Tirupati and Tirumala, which is a world renowned Pilgrim centre located in ChittoorDistrict in the state of Andhra Pradesh. The chef’s perception on the four aspects vizhealth, nutrition, practices and consumer concerns were assessed in the context of menu planning and food service. The results revealed that in both locations the chef’s of hotels showed a better Perception in all four areas when compared to the Chef’s of restaurants. The differences were significant out 0.01 percent. The findings also revealed that chefs of restaurants at Tirumala showed good perceptions in the areas of nutrition and health. A low level of perceptions was evident in practicing nutrition and consumer concern. Chef’s in general believed that nutrition is important in menu planning. Effortshowever, need to be to facilitate nutrition related practices in the Food cateringunits and increase consumer awareness and healthy eating out habits of consumers. Chefs thus, in strongly believed that nutrition is important in menu planning in food catering institutions, there is a great need to create facilitating environments to convert perceptions into sustaining practices.
最近,我们的人口中外出就餐的事件急剧增加。为了满足需求,全国各地建立的食品服务单位有所增加。在西方世界,消费者习惯于外出就餐。鉴于肥胖和与之相关的慢性健康问题,如心血管疾病、冠心病、糖尿病等的发病率有所增加,我们正重点关注健康的外出饮食习惯。这项研究调查了蒂鲁帕蒂和蒂鲁马拉的一些酒店和餐馆的厨师,蒂鲁马拉是世界著名的朝圣者中心,位于安得拉邦的奇图尔区。在菜单规划和食品服务的背景下,评估了厨师对健康、营养、实践和消费者关注四个方面的看法。结果显示,在这两个地区,酒店的厨师在这四个方面都比餐厅的厨师表现得更好。差异在0.01%以内显著。调查结果还显示,蒂鲁马拉餐馆的厨师在营养和健康方面表现得很好。在实践营养和消费者关注方面,认知水平很低。厨师们普遍认为营养在菜单设计中很重要。然而,需要努力促进食品餐饮单位的营养相关实践,提高消费者的意识和健康的外出就餐习惯。因此,厨师们坚信营养在食品餐饮机构的菜单规划中很重要,因此非常需要创造便利的环境,将观念转化为可持续的实践。
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引用次数: 1
Quality Evaluation of Pasta Fortified With Roasted Sesame Flour 烤芝麻粉强化面食的质量评价
Pub Date : 2017-07-01 DOI: 10.9790/2402-1107012934
Oluwatoyin Habibat Animashaun, O. Olorode, K. S. Sofunde, M. Idowu
Pasta is a stable food product that is eaten by both young and old in many parts of the world. The quality characteristics of pasta fortified with roasted sesame flour were evaluated. Sesame seeds were processed into flour after roasting and incorporated into wheat flour in ratio of 100:0, 90:10, 80:20, 70:30, 60:40 and 50:50 (wheat: roasted sesame flour). The functional, proximate and mineral composition of flour blends and sensory attribute of spaghetti produced from the blends were carried out according to standard methods. The result of functional properties revealed that values for flour blend with 50% sesame flour had the highest value for water absorption capacity, solubility and gelatin and least value for bulk density BD and swelling capacity. The result of proximate composition showed that values for protein, fat, fibre, ash, moisture and carbohydrate ranged from 6.78 to 15.16%, 1.05 to 1.33%, 0.23 to 0.68%, 1.29 to 1.98%, 10.50 to 13.11% and 70.27 to 76.50 respectively. From the result obtained, it can be concluded that acceptable spaghetti with high nutritional and sensory attributes can be produced from the blends of wheat-sesame at 20% inclusion level, which can help in solving the lingering problem of protein malnutrition.
意大利面是一种稳定的食品,在世界许多地方,年轻人和老年人都吃它。对烤芝麻粉强化面食的品质特性进行了评价。芝麻经烘烤后加工成面粉,并按100:0、90:10、80:20、70:30、60:40和50:50的比例加入小麦粉中(小麦:烤芝麻粉)。按照标准方法测定了共混面粉的功能成分、近似成分和矿物成分,以及由共混面粉制成的意面的感官特性。结果表明,添加50%芝麻粉的面粉的吸水率、溶解度和明胶性能最高,容重BD和溶胀性能最低。近似组成结果表明,蛋白质、脂肪、纤维、灰分、水分和碳水化合物含量分别为6.78 ~ 15.16%、1.05 ~ 1.33%、0.23 ~ 0.68%、1.29 ~ 1.98%、10.50 ~ 13.11%和70.27 ~ 76.50。综上所述,20%添加量的小麦-芝麻混合料可生产出高营养和感官属性的可接受意面,有助于解决长期存在的蛋白质营养不良问题。
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引用次数: 2
Food Adulteration and Contamination-A Catastrophe 食品掺假和污染——一场灾难
Pub Date : 2017-07-01 DOI: 10.9790/2402-1107016270
G. Vyralakshmi, G. Jayasheela
In Chennai, Tamil Nadu food were contaminated and adulterated which are consumed by the consumers a those-hold level, food service firms such as hotels,catering service, etc. and especially when the street food are consumed. Food colouring are done by using highly chemical mixed colours which are not permitted to use in food items which are not good for our health and it may lead to food-borne diseases. Contamination of mycotoxins, metals and pesticides in food items which we consume daily such as milk,rice,etc which were found with highly toxic and carcinogenic. More number of people die every year due to food borne diseases. In order to prevent such things, food safety measures are prominent with an aim to prevent the consumers from health hazards and empowering the regulatory system.It is desirable to prevent adulteration and contamination of food items, if the consumers are aware of health hazards.
在金奈,泰米尔纳德邦的食品被污染和掺假,这些食品被消费者消费,食品服务公司,如酒店,餐饮服务等,特别是当街头食品被消费时。食用色素是使用高度化学混合的色素制成的,不允许在食物中使用,因为这些色素对我们的健康有害,并可能导致食源性疾病。我们日常食用的食物,例如牛奶、大米等,受到霉菌毒素、金属和杀虫剂的污染,这些食物被发现具有剧毒和致癌性。每年有越来越多的人死于食源性疾病。为了防止这样的事情,食品安全措施是突出的,目的是防止消费者的健康危害,并赋予监管系统权力。如果消费者意识到对健康的危害,防止食品掺假和污染是可取的。
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引用次数: 4
Available Molybdenum Forms Distribution in some Soil Profiles of Different Locations of Southwestern Nigeria. 尼日利亚西南部不同地区土壤剖面中有效钼形态的分布。
Pub Date : 2017-07-01 DOI: 10.9790/2402-1106034245
O. Oseni, A. Taiwo, O. IjaolaT., O. S. Shoyemi
Available molybdenum in soil profiles of twelve (12) different locations of Ogun and Oyo States in South Western Nigeria was investigated to know its status with respect to soil depths.Soil samples derived from sedimentary and igneous/metamorphic rockscomprising of Alfisols (8 profiles) and Ultisols (4 profiles) were collected at four (4) different soil depths of 0-20 cm, 20-40 cm, 40-60 cm and 60-80 cm respectively from savanna and forest vegetational zones. Soil physic-chemical properties of pH, organic carbon, total nitrogen, CEC, clay, silt, and sand were determined using standard methods and correlated with the available molybdenum. The results showed that available molybdenum was positively correlated with pH, silt and clay (r = 0.403**, r = 0.072** and r = 0.025**) and also negatively correlated organic carbon, with total nitrogen, CEC and sand (r = -0.092**, r = 0.114**, r = 0.253** and r = -0.061**) respectively. The status of available Mo fell above the critical limits of 0.05 and 0.1ppm and as a result, Farmers are advised not to apply molybdenum fertilizers in these soils so as not to cause environmental pollution and soil toxicity.
对尼日利亚西南部奥贡州和奥约州12个不同地点的土壤剖面中可用钼进行了调查,以了解其与土壤深度的关系。在热带稀树草原和森林植被带分别采集了0-20 cm、20-40 cm、40-60 cm和60-80 cm 4种不同土壤深度的沉积和火成岩/变质岩样品,包括Alfisols(8个剖面)和Ultisols(4个剖面)。采用标准方法测定了土壤pH、有机碳、全氮、CEC、粘土、粉土和砂的理化性质,并与有效钼进行了相关性分析。结果表明:有效钼与pH、粉土和粘土呈显著正相关(r = 0.403**、r = 0.072**和r = 0.025**),与有机碳、总氮、CEC和砂呈显著负相关(r = -0.092**、r = 0.114**、r = 0.253**和r = -0.061**)。有效钼含量高于0.05和0.1ppm的临界限值,建议农民不要在这些土壤中施用钼肥,以免造成环境污染和土壤毒性。
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引用次数: 0
Optimization and Physico-Chemical Characterization of a Bacteriocin Produced By Marine Lactobacillus Rhamnosus L43 海洋鼠李糖乳杆菌L43产细菌素的优化及理化特性研究
Pub Date : 2017-07-01 DOI: 10.9790/2402-1107012128
Bharti Prakash Wadekar, S. M. Dharmadhikari
Objective: To optimize bacteriocin producing medium and their characterization. Methods: Lactobacillus strain was isolated from marine water, Thirumullavaram beach, Kerala using MRS broth (Hi-Media, India) at 37 0 C for 48 hrs. The isolate was identified by 16S rRNA sequencing and phylogenetic analysis. Optimization of physiological parameters and carbon, nitrogen sources. Produced crude bacteriocin and antagonistic characteristics of bacteriocin against Klebsiella pneumoniae MTCC535 were studied by agar well diffusion method. Bacteriocin stability in different temperature and enzyme. Result: Lactobacillus rhamnosus strain isolated from marine water. Lactobacillus isolate was sequenced which shown high similarity with reference strain Lactobacillus rhamnosus ZY Accession number KC012630.1. Bacteriocin produced at 30 0 C, pH 7, 18 hrs. incubation time with 2% inoculum size and 2% NaCl concentration. It produced in presence of lactose and peptone. All the data validated by statistical analysis using paired t-test by MINITAB 14. This bacteriocin was stable at 100 0 C for 20 min, and it is in proteinaceous nature. Conclusion: These results indicated the potent strain produce bacteriocin. Maximum bacteriocin production was observed at 30 o C, 18 hrs. incubation, pH 7.0, 2% NaCl solution.
目的:优化细菌素生产培养基及其性能。方法:从喀拉拉邦Thirumullavaram海滩的海水中分离出一株乳酸菌,采用MRS肉汤(Hi-Media, India)在37℃下培养48 h。通过16S rRNA测序和系统发育分析对分离物进行了鉴定。生理参数优化及碳、氮源优化。采用琼脂孔扩散法研究了细菌素粗产物及其对肺炎克雷伯菌MTCC535的拮抗特性。细菌素在不同温度和酶下的稳定性。结果:从海水中分离到鼠李糖乳杆菌。对分离得到的乳酸菌进行了测序,结果表明该菌株与参考菌株鼠李糖乳杆菌ZY具有较高的相似性,测序号为KC012630.1。细菌素在30℃,pH 7, 18小时下产生。接种量为2%,NaCl浓度为2%。它在乳糖和蛋白胨存在的情况下产生。所有数据均采用MINITAB 14的配对t检验进行统计分析。该细菌素在100℃下稳定保存20 min,具有蛋白质性质。结论:本实验结果表明该菌株能产生细菌素。在30℃、18小时时细菌素产量最大。孵育,pH 7.0, 2% NaCl溶液。
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引用次数: 1
The Permanent Agriculture as a Means of Harmony between Nature Cycle and Human Being 永续农业:实现自然循环与人类和谐的手段
Pub Date : 2017-07-01 DOI: 10.9790/2402-1106034655
G. Akabane, José Roberto Kassai, António César Galhardi
The transformational spirit of evolution made us as human beings that shaping both the landscapes we inhabit and the universe of thought we entertain; it moves along our experiences, everything that we observe, reflect and engage. This research in exploratory case format was carried out on a farm in Bolivia through the research project used to detect the perceptions, opinions and feelings of the participants based on their experiences for decades with permanent agriculture. It tried to get a thorough understanding essences of the central phenomenon that follows the teaching guide modified the principle of permaculture Brown (2009) describing the essential properties of permanent agriculture. As result reflections, the agricultural system fundamental transformation requires a change of perspective and cultural awareness around human interrelations with the natural and industrial world. It also challenges people to take action in their communities to adopt sustainable agricultural practices that meet a human needs on a scale practice can transform way we view our relationship for both earth and each other’s. And this success case with favorable performances especially in economic aspects shows a transition path for models that address the social, environmental and good governance.
进化的变革精神使我们成为人类,它既塑造了我们居住的景观,也塑造了我们思考的宇宙;它伴随着我们的经历,我们观察、思考和参与的一切。本研究以探索性案例的形式在玻利维亚的一个农场进行,通过研究项目来检测参与者基于他们几十年来从事永久性农业的经验的看法、意见和感受。它试图得到一个彻底的理解的核心现象的本质,遵循教学指南修改的原则的永久农业布朗(2009)描述永久农业的基本属性。因此,农业系统的根本转型需要改变人类与自然和工业世界相互关系的观点和文化意识。它还要求人们在他们的社区采取行动,采用可持续的农业做法,以满足人类的大规模需求,这种做法可以改变我们看待地球和彼此关系的方式。这个在经济方面表现良好的成功案例为解决社会、环境和良好治理的模式指明了一条过渡之路。
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引用次数: 1
Quality Assessment of Sweetmeat (Rosogolla) Of Dhaka and Tangail Region of Bangladesh 孟加拉国达卡和坦盖尔地区甜肉(Rosogolla)质量评价
Pub Date : 2017-07-01 DOI: 10.9790/2402-1107010611
U. Prodhan, Md. jahangir Alam, A. Sultana, Md Harun Rashid, T. Das, N. Akter, M. M. Rahman
An experimental study was design to assess the nutritional quality of the Rosogolla available in Dhaka and Tangail region of Bangladesh. Sweetmeats were collected from a branded shop in Mirpur road and non-branded Rosogolla in Dhaka city area and a branded shop in pachani bazar and non-branded Rosogolla available at old bus-stand of Tangail district area. Quality of the Rosogolla was evaluated by chemical, microbial and hazard analysis. It was found that quality differed according to the manufacturers of Rosogolla. The chemical analysis score implies that moisture, fat, protein, total sugar and ash content varied significantly (p<0.05) among four different types of Rosogolla. The mineral content i.e. Na, K, Mn, Fe, Zn and Ca in all samples were statistically significant (p<0.05). The toxic heavy metals such as As, Cr, Hg and Pb were not detected in all sample. It was found that the standard plate count in all sample were higher than the maximum permissible level of Bangladesh Standard and Testing Institute (BSTI) standard except R2 sample. Total coliform and total fungal count in all samples also found in unsatisfactory level. About 50% sample contained Salmonella and Staphylococcus aureus which may be a hazard to consumer as they are pathogenic bacteria.
设计了一项实验研究,以评估孟加拉国达卡和坦盖尔地区可用的Rosogolla的营养质量。糖果是从达卡市区Mirpur路的一家品牌商店和非品牌Rosogolla以及Tangail区旧公交站的pachani bazaar的一家品牌商店和非品牌Rosogolla中收集的。采用化学分析、微生物学分析和危害分析等方法对其质量进行评价。研究发现,不同厂家的Rosogolla的质量不同。水分、脂肪、蛋白质、总糖和灰分含量在4种不同品种间差异显著(p<0.05)。各样品中Na、K、Mn、Fe、Zn、Ca的矿物质含量均有统计学意义(p<0.05)。所有样品均未检出砷、铬、汞、铅等有毒重金属。结果发现,除R2样品外,所有样品中标准板计数均高于孟加拉国标准与检测协会(BSTI)标准的最大允许水平。所有样品的总大肠菌群和总真菌数量也未达到不合格水平。大约50%的样本含有沙门氏菌和金黄色葡萄球菌,它们是致病菌,可能对消费者造成危害。
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引用次数: 5
Biodegradation of Azo Dyes Using Bacillus Megaterium and Its Phytotoxicity Study 巨型芽孢杆菌降解偶氮染料及其植物毒性研究
Pub Date : 2017-07-01 DOI: 10.9790/2402-1107011220
Lekha R, Dr.Maleeka Begum, Dr.R. Ragunathan
The release of azo dyes from various textile industries contributes a serious problem to the environment. The biological method of degrading these dyes is preferred over chemical and physical methods. In the present study, the potentiality of using Bacillus megaterium (MTCC 8371) for degrading four different azo dyes, namely methylene blue, methyl red, congo red, and orange –G, under optimum conditions were studied. Kirk’s media was used for the degradation study and it was found out that the organism degraded the dye best in Kirk’s media on the fifth day of incubation under neutral pH at 40oC. The degradation was estimated by using UV-VIS spectrophotometer. FTIR study was also carried out to find out the degraded compounds in the samples. TLC was performed to find out the Rƒ value of the degraded dyes. The molecular weight of laccase enzyme was identified by SDS-PAGE and the degradation was further conformed by laccase assay. GC –MS analysis was also performed for the further confirmation of degradation.
各种纺织工业释放的偶氮染料对环境造成了严重的问题。生物降解染料的方法优于化学和物理方法。在最佳条件下,研究了巨芽孢杆菌(MTCC 8371)对亚甲基蓝、甲基红、刚果红和橙-G四种偶氮染料的降解潜力。采用Kirk’s培养基进行降解研究,发现在中性pH、40℃条件下,培养第5天,微生物在Kirk’s培养基中对染料的降解效果最好。用紫外-可见分光光度计对其降解进行了评价。并用FTIR研究了样品中降解的化合物。采用薄层色谱法测定降解染料的rf值。通过SDS-PAGE鉴定了漆酶的分子量,并通过漆酶测定进一步证实了漆酶的降解。GC -MS分析进一步证实了降解。
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引用次数: 9
Several “Organic” Vineyards, Manuered And Fertilized With Special Cover Crops, Produce Organic Wine: Its Habitual Consumption Can Result Letal To Man 几个“有机”葡萄园,用特殊的覆盖作物施肥和施肥,生产有机葡萄酒:它的习惯性消费可能导致致命的人
Pub Date : 2017-07-01 DOI: 10.9790/2402-1107018386
L. Martini
It is my specific intention to demonstrate that “Organic wine”, that has imposed a crucial fashion in several countries of all over the world, letting its cost grow hawful, may be severally perilous for Human Health, since the cover crops used to manure roots of vine can contain, beside hay or trifolium, even vetch, lupins and faba vicia minor (field beans), that contain vicine, a glycoside that can drive to anemia, favism and finally, if not tempestively treated, death of the habitué of wine tasting or drinking. I have detected great percentages of vicine in the fresh young leaves, in the first unripe grapes and finally in the Organic wine itself that was manured with lupins, vetch and field beans.
我的具体意图是要证明,“有机葡萄酒”在世界上几个国家成为一种至关重要的时尚,让其成本飙升,可能对人类健康构成多重危险,因为用于给葡萄藤根部施肥的覆盖作物,除了干草或三叶草外,甚至包括紫薇、羽扇豆和蚕豆(菜豆),都含有疫苗,一种能导致贫血的糖苷,蚕豆病,最后,如果不经过迅速处理,品酒或饮酒习惯的消亡。我在新鲜的嫩叶中,在第一批未成熟的葡萄中,最后在用羽扇豆、紫薇和菜豆施肥的有机葡萄酒中,都发现了很大比例的疫苗。
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引用次数: 0
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IOSR Journal of Environmental Science, Toxicology and Food Technology
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