Study On the Biogas Yielding Potential of a Portable Bioreactor Using a Blend of Corn Cob and Rice Chaff Mixed With Goat and Dog Dungs

N. P. Okolie, A. Onifade, K. OladunmoyeM., D. Adegunloye
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Abstract

The study is to investigate the viability of corncob and rice chaff co-digested with goat and dog dungs in the production of biogas and the PCR detection of the methanogenic bacteria involved. The study was carried out at mesophillic condition between (29.5 –33 0 C) in a mini laboratory digester fabricated using guage 16 metal sheets with 80L capacity for a 90 days retention time. Corn cob and rice chaff were shredded and mixed with water with ratio 4:1 and 3:1 (waste to water) respectively and mixed with goat and dog dungs with ratio 2:1 (waste to water) and digested anaerobically. The proximate analysis of all the substratesused were carried out and the result shows a C:N (carbon, nitrogen ratio) that is appropriate for the biogas production. The result shows a yield of 15L after 20days at temperature 31 0 C and pH 6.2, there was a drop in the yield and then an increase from the 40th day of digestion. The cumulative production was 37L at temperature 31 0 C and pH 5.9. The bacteria isolated from the samples includes; E. coli, Proteusspp, Klebsiellapneumoniae,Serratia. spp, Flavimonas spp. The result revealed that this combination of substrates can yield significantly biogas.
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玉米芯、稻壳与羊粪、狗粪混合的便携式生物反应器产气潜力研究
本研究旨在探讨玉米芯和稻壳与羊粪和狗粪共消化在沼气生产中的生存能力及相关产甲烷菌的PCR检测。研究在(29.5 -33 0℃)的中温条件下,在一个小型实验室消化池中进行,该消化池使用容量为80L的16规格金属板制造,保留时间为90天。将玉米芯和稻壳切碎,分别以4:1和3:1(废水比)的比例与水混合,并以2:1(废水比)的比例与羊粪和狗粪混合厌氧消化。对所使用的所有基质进行了近似分析,结果表明C:N(碳氮比)适合沼气生产。结果表明,在温度为310℃、pH为6.2条件下,发酵20天后产量为15L,从消化第40天开始产量先下降后上升。在温度310℃、pH 5.9条件下,累计产量为37L。从样品中分离出的细菌包括;大肠杆菌,变形杆菌,克雷伯氏肺炎,沙雷氏菌。结果表明,这种底物组合可以产生显著的沼气。
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