Validation and development of UV-Vis spectrophotometer analysis methods for alpha-tocopherol acetate

Arif Al Iman, Winni Auli, S. Sukrasno
{"title":"Validation and development of UV-Vis spectrophotometer analysis methods for alpha-tocopherol acetate","authors":"Arif Al Iman, Winni Auli, S. Sukrasno","doi":"10.20885/jif.vol19.iss1.art8","DOIUrl":null,"url":null,"abstract":"Background: The COVID-19 pandemic had caused the consumption of vitamins in the community to increase with the aim of maintaining immunity in the body. Objective: This study aims to develop an analysis method with a UV-Vis spectrophotometer, which is considered an alternative analysis method that is easier, more practical, and cheaper. Method: Methods for analyzing alpha-tocopherol acetate are validated through specificity tests, linearity measurements, precision tests, accuracy tests, determination of detection limits, determination of quantitation limits, and determination of range using ethanol p.a solvent and UV-Vis Spectrophotometer instruments. Then, examine the alpha tocopherol acetate content of vitamin E dietary supplements. Results: The specificity test showed that matrix had no significant effect on the analysis, the linearity test met the requirements with a concentration range of 20–60 ug/mL (r = 0.9991), the accuracy test was 99.897-100.315%, intraday and interday precision tests were obtained with %RSD less than 2%, the LOD value was 1.859 ug/mL and the LOQ value was 5.635 ug/mL. The determination of levels of vitamin E in dietary supplements showed levels for brand A of 100.508% and brand B of 100.089%. Conclusion: The analytical method developed had met the validation requirements, and the levels of vitamin E food supplements had met the USP requirements. Keywords: method validation, UV-Vis spectrophotometer, alpha tocopherol acetate","PeriodicalId":32369,"journal":{"name":"Kartika Jurnal Ilmiah Farmasi","volume":"76 1 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2023-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Kartika Jurnal Ilmiah Farmasi","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.20885/jif.vol19.iss1.art8","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

Background: The COVID-19 pandemic had caused the consumption of vitamins in the community to increase with the aim of maintaining immunity in the body. Objective: This study aims to develop an analysis method with a UV-Vis spectrophotometer, which is considered an alternative analysis method that is easier, more practical, and cheaper. Method: Methods for analyzing alpha-tocopherol acetate are validated through specificity tests, linearity measurements, precision tests, accuracy tests, determination of detection limits, determination of quantitation limits, and determination of range using ethanol p.a solvent and UV-Vis Spectrophotometer instruments. Then, examine the alpha tocopherol acetate content of vitamin E dietary supplements. Results: The specificity test showed that matrix had no significant effect on the analysis, the linearity test met the requirements with a concentration range of 20–60 ug/mL (r = 0.9991), the accuracy test was 99.897-100.315%, intraday and interday precision tests were obtained with %RSD less than 2%, the LOD value was 1.859 ug/mL and the LOQ value was 5.635 ug/mL. The determination of levels of vitamin E in dietary supplements showed levels for brand A of 100.508% and brand B of 100.089%. Conclusion: The analytical method developed had met the validation requirements, and the levels of vitamin E food supplements had met the USP requirements. Keywords: method validation, UV-Vis spectrophotometer, alpha tocopherol acetate
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
紫外-可见分光光度法测定α -生育酚醋酸酯方法的验证与发展
背景:COVID-19大流行导致社区维生素消费增加,目的是维持体内免疫力。目的:本研究旨在建立一种简便、实用、经济的紫外可见分光光度计分析方法。方法:通过特异性试验、线性测量、精密度试验、准确度试验、检出限测定、定量限测定、范围测定等方法,对α -生育酚醋酸酯的分析方法进行了验证。然后,检测维生素E膳食补充剂中α -生育酚醋酸酯的含量。结果:特异性试验表明基质对分析无显著影响,线性试验满足要求,浓度范围为20 ~ 60 ug/mL (r = 0.9991),准确度试验为99.897 ~ 100.315%,日内、日间精密度试验RSD < 2%, LOD值为1.859 ug/mL, LOQ值为5.635 ug/mL。对膳食补充剂中维生素E含量的测定显示,A牌维生素E含量为100.508%,B牌维生素E含量为100.089%。结论:所建立的分析方法符合验证要求,维生素E食品补充剂的含量符合美国药典的要求。关键词:方法验证,紫外可见分光光度计,醋酸α生育酚
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
自引率
0.00%
发文量
0
审稿时长
16 weeks
期刊最新文献
Pengetahuan, sikap, dan perilaku konsumsi suplemen dan obat tradisional mahasiswa Universitas Jember saat pandemi COVID-19 Hubungan Tingkat Pengetahuan dengan Perilaku Swamedikasi Obat Golongan NSAID di Apotek X Banjarmasin EFEKTIVITAS KOMBINASI EKSTRAK ETANOL 70% DAUN RAMANIA (Bouea macrophylla Griffith) dan GLIBENKLAMID DENGAN METODE INTOLERANSI GLUKOSA PERBANDINGAN KADAR GLUKOSA DARAH DAN HBA1C PADA INSULIN GLARGINE DAN DETEMIR PASIEN DIABETES MELITUS TYPE 2 DI RUMAH SAKIT UMUM DAERAH ULIN BANJARMASIN The study of conformity of the pharmaceutical service in Puskesmas Bogor City towards PMK No. 74, 2016
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1