Simultaneous determination of moxifloxacin and cefixime by first and ratio first derivative ultraviolet spectrophotometry

M. Attimarad, Bander E. Al-Dhubiab, I. Alhaider, Anroop B Nair, N. Sreeharsha, K. MueenAhmed
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引用次数: 1

Abstract

Background: The new combination of moxifloxacin HCl and cefixime trihydrate is approved for the treatment of lower respiratory tract infections in adults. At initial formulation development and screening stage a fast and reliable method for the dissolution and release testing of moxifloxacin and cefixime were highly desirable. The zero order overlaid UV spectra of moxifloxacin and cefixime showed >90% overlapping. Hence, simple, accurate precise and validated two derivative spectrophotometric methods have been developed for the determination of moxifloxacin and cefixime. Methods: In the first derivative spectrophotometric method varying concentration of moxifloxacin and cefixime were prepared and scanned in the range of 200 to 400 nm and first derivative spectra were calculated (n = 1). The zero crossing wavelengths 287 nm and 317.9 nm were selected for determination of moxifloxacin and cefixime, respectively. In the second method the first derivative of ratio spectra was calculated and used for the determination of moxifloxacin and cefixime by measuring the peak intensity at 359.3 nm and 269.6 nm respectively. Results: Calibration graphs were established in the range of 1–16 μg /mL and 1–15 μg /mL for both the drugs by first and ratio first derivative spectroscopic methods respectively with good correlation coefficients. Average accuracy of assay of moxifloxacin and cefixime were found to be 100.68% and 98 93%, respectively. Relative standard deviations of both inter and intraday assays were less than 1.8%. Moreover, recovery of moxifloxacin and cefixime was more than 98.7% and 99.1%, respectively. Conclusions: The described derivative spectrophotometric methods are simple, rapid, accurate, precise and excellent alternative to sophisticated chromatographic techniques. Hence, the proposed methods can be used for the quality control of the cited drugs and can be extended for routine analysis of the drugs in formulations.
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一阶导数和比例一阶导数紫外分光光度法同时测定莫西沙星和头孢克肟的含量
背景:盐酸莫西沙星和三水合头孢克肟的新组合被批准用于治疗成人下呼吸道感染。在最初的处方开发和筛选阶段,迫切需要一种快速可靠的方法来检测莫西沙星和头孢克肟的溶出和释放。莫西沙星和头孢克肟的零阶重叠紫外光谱重叠度大于90%。因此,建立了两种导数分光光度法测定莫西沙星和头孢克肟的方法。方法:采用一阶导数分光光度法制备不同浓度的莫西沙星和头孢克肟,在200 ~ 400 nm范围内扫描,计算一阶导数光谱(n = 1),分别选择零交叉波长287 nm和317.9 nm测定莫西沙星和头孢克肟。第二种方法计算比值光谱的一阶导数,分别在359.3 nm和269.6 nm处测量峰强度,用于莫西沙星和头孢克肟的测定。结果:采用一阶导数法和比例一阶导数法分别在1 ~ 16 μg /mL和1 ~ 15 μg /mL范围内建立了两种药物的标度图,相关系数较好。莫西沙星和头孢克肟测定的平均准确度分别为100.68%和98.93%。日间和日间测定的相对标准偏差均小于1.8%。莫西沙星和头孢克肟的回收率分别大于98.7%和99.1%。结论:所建立的导数分光光度法简便、快速、准确、精密度高,可替代复杂的色谱技术。因此,所提出的方法可用于所引用药物的质量控制,并可扩展到制剂中药物的常规分析。
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