Integrated Management of Linseed Blight by Green Silver Nanoparticles Synthesized from Fruit and Leaves Extract of Morinda citrifolia L.

Abstract

In the present work, an attempt has been made to synthesize green silver nanoparticles by using medicinal plant Morinda citrifolia. Biologically synthesized nanoparticles have been used widely in the various fields. Morinda fruit and leaves extract was used to reduce silver nitrate to synthesized silver nanoparticles. The peak showed at 425nm for fruits and 425 to 450nm for leaves sample by Uv-vis spectrophotometer and FESEM analysis reveales the spherical shaped nanoparticles with size ranging between 25-60 nm and 40-60nm respectively. These synthesized nanoparticles showed inhibitory activity against Alternaria lini a linseed blight pathogen. The 3% carbendazim showed inhibition of growth of fungi A.lini, Whereas Carbendazim combined with silver nanoparticles significantly inhibit the growth of A.lini. This will help to reduce the percentage of carbendazim and also help to minimize the fungicide residues. Thus these nanoparticles synthesized from M. citrifolia fruits and leaves might be used in agricultural industries for the development of efficient fungicide. This will helps to reduce the amount of fungicide and residual biomagnifications in food chain. © 2019 iGlobal Research and Publishing Foundation. All rights reserved. Cite this article as: Koparde, S.B.; Gaikwad, D.K. Integrated management of linseed blight by green silver nanoparticles synthesized from fruit and leaves extract of Morinda citrifolia L. Indo Global J. Pharm. Sci., 2019; 9(2): 73-76. DOI: http://doi.org/10.35652/IGJPS.2019.9204 . Indo Global Journal of Pharmaceutical Sciences, 2019; 9(2): 73-76 74 minutes. The extract was cooled and filtered using whatman no. 1 filter paper and used for the further reaction. Silver nanoparticles synthesis For the synthesis of silver nanoparticles M. citrifolia fruit and leaves extract was reacted with silver nitrate solution. 10 ml extract added to the 90 ml silver nitrate solution. This reaction mixture containing conical flask was kept in dark condition at room temperature. The bioreduction of silver ions by fruit and leaves extract was scrutinized by measuring the Uv-vis spectra of the reaction medium at 200-800nm. Complete bioreduced sample was centrifuged at 10000 rpm for 15 minutes, redispersed the pellet in double distilled water and again centrifuged. This process was repeated for 2-3 times for obtaining silver nanoparticles free from any unwanted residue [8 and 13].