Spectrophotometric Determination of Thiamine hydrochloride Via Oxidative Coupling Reaction Using 4-Aminoantipyrine

R. Al-Luhaiby, Mohammed S. Al-Enizzi
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Abstract

A sensitive spectrophotometric method has been developed for the determination of thiamine hydrochloride by oxidative coupling reaction of thiamine with the reagent 4-aminoantipyrine (4-AAP) in the presence of copper sulphate as oxidizing agent in alkaine medium forming a reddish brown colour. The product show maximum absorption at 335 nm. The molar absorptivitiy is 26410.59 l/mol.cm for concentrations obeyed Beer’s law in the range 0.2-18 μg.ml. The recovery was 100.93 % with relative standard deviation < 2.0 % for thiamine hydrochloride.Thiamine and reagent 4-AAP product was formed in the ratio of 1:1. The stability constant of the product was 3.39×10 l.mol for thiamine hydrochloride indicating the good stability of this product. The optimum conditions for full colour development are described and the proposed method was applied successfully for determination of thiamine in the pharmaceutical preparation (Neurorubine). The common excipients used as additives in pharmaceutical do not interfere in the proposed method.
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4-氨基安替比林氧化偶联分光光度法测定盐酸硫胺素
以硫酸铜为氧化剂,在碱介质中,硫胺素与4-氨基安替比林(4-AAP)氧化偶联反应,形成红棕色,建立了测定盐酸硫胺素的灵敏分光光度法。该产品在335 nm处有最大吸收。摩尔吸光度为26410.59 l/mol。在0.2 ~ 18 μg.ml范围内,浓度符合比尔定律。盐酸硫胺素的回收率为100.93%,相对标准偏差< 2.0%。硫胺素与试剂4-AAP产物按1:1的比例生成。盐酸硫胺素的稳定常数为3.39×10 l.mol,表明该产品具有良好的稳定性。描述了全色显色的最佳条件,并成功地应用于药物制剂(neurorrubine)中硫胺素的测定。在制药中作为添加剂使用的常见赋形剂不干扰所提出的方法。
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