EFFICIENCY OF OILY AND POLYELECTROLYTE ADJUVANTS IN CASE OF RABBIT HYPERIMMUNIZATION WITH DIMETHYLSULFOXIDE ANTIGEN OF PSEUDOTUBERCULOSIS MICROORGANISM

Abstract

A comparative evaluation of two adjuvants: polyazolidinammonium modified with iodine hydrate ions (PAAH) and complete Freud's adjuvant (CFA) was conducted. The assessment was carried out in the process of hyperimmunization of rabbits with a mixture of an adjuvant and dimethyl sulfoxide antigen (DA) of a pseudotuberculous microbe. Both adjuvants stimulated antibody genesis and allowed to obtain hyperimmune sera with high antibody titers. However, CFA showed a slightly higher activity (1:819200) than PAAH (1:409600). The most pronounced stimulating effect of adjuvants was expressed at low doses of DA (0.2-1 mg/rabbit). Large doses of the antigen (16.0 mg/rabbit) enabled to obtain sera with a high antibody titer without adjuvants. The stimulating effect of DA-PAAH and DA-CFA complexes on antibody genesis is associated with the rate and quality of lymphocyte differentiation, and to a lesser extent with an increase of their number. PAAH showed a weak irritating effect on body tissues at the injection spot, and CFA had a strong irritating effect. The application of CFA led to formation of many painful indurations in the subcutaneous tissue of rabbits, which did not resolve between immunizations. Thus, usage of PAAH as an adjuvant in hyperimmunization of rabbits with DA of the pseudotuberculous microbe is potentially productive and without CFA disadvantages.