Novel method of hydrolysed collagen extraction from hide trimming waste

IF 0.7 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY international food research journal Pub Date : 2023-04-28 DOI:10.47836/ifrj.30.2.08
F. Purba, O. Suparno, M. S. Rusli, I. Fatimah
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Abstract

Hide trimming waste is a by-product of tannery. Collagen is the main structural protein in hide trimming waste (70%), and extensively utilised in numerous industries including food, non-food, cosmetics, and medical. Research related to the development of hydrolysed collagen (h-collagen) extraction methods from cowhide, especially from the hide trimming waste, is still limited. The present work thus aimed to develop a four-step method for extracting h-collagen from hide trimming waste, and examine the product's properties. The present work successfully developed a method for extracting h-collagen from trimming waste with a 20.35% yield. Analysis of molecular weight, FTIR, and amino acid composition confirmed that the product was h-collagen with a molecular weight of 16 - 23 kDa. This h-collagen had higher antioxidant activity than commercial h-collagen, with an IC50 value of 238.5 ppm.
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从剥皮废料中提取水解胶原蛋白的新方法
削皮废料是制革厂的副产品。胶原蛋白是削皮废料的主要结构蛋白(占70%),广泛应用于食品、非食品、化妆品、医疗等众多行业。从牛皮中提取水解胶原蛋白(h-胶原蛋白)的方法,特别是从削皮废料中提取胶原蛋白的研究仍然有限。因此,本研究旨在开发一种四步法从削皮废料中提取h-胶原蛋白,并研究产品的性质。本工作成功地开发了一种从切屑中提取h-胶原蛋白的方法,收率为20.35%。分子量、FTIR和氨基酸组成分析证实产物为h-胶原蛋白,分子量为16 - 23kda。该h-胶原具有较高的抗氧化活性,IC50值为238.5 ppm。
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来源期刊
international food research journal
international food research journal Agricultural and Biological Sciences-Food Science
CiteScore
1.40
自引率
0.00%
发文量
75
期刊介绍: The International Food Research Journal (IFRJ) publishes papers in English, six (6) issues a year with the coverage of: Food Science and Technology Nutrition and Dietetics Agriculture, multidisciplinary Chemistry, multidisciplinary The scope of the Journal includes: Food Science, Food Technology and Food Biotechnology Product Development and Sensory Evaluation Food Habits, Nutrition, and Health Food Safety and Quality Food Chemistry, Food Microbiology, Food Analysis and Testing Food Engineering Food Packaging Food Waste Management Food Entrepreneur Food Regulatory Post-Harvest Food Management Food Supply Chain Management Halal Food and Management
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