COMPARATIVE IN-VITRO ANTIOXIDANT POTENTIAL OF SOME HERBAL PLANTS

YMER Digital Pub Date : 2022-08-20 DOI:10.37896/ymer21.08/70
Beekesh Yadav, R. Mittal, N. Kumar
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Abstract

The use of plants in treatment of burns, dermatophytes and infectious diseases is common in traditional medicine. Based on ethno pharmacological and taxonomic information, antibacterial activities of aqueous and ethanolic extracts of some medicinal plants were determined by in vitro by agar diffusion-method against some human pathogenic bacteria. The leaves of five different plants, belonging to the different family and which have some ethnomedicinal applications were studied for antibacterial activity. Powdered leaf materials of all selected plants were extracted with aqueous and methanol. The solvent extracts were evaporated to dryness using rotary flash evaporator. Dry residue was dissolved in ethanol (1:10 w/v) and tested for antibacterial activity. The antibacterial screening of aqueous and methanol extract carried out in vitro on the following bacteria viz., Bacillus cereus, Bacillus megaterium, Bacillus subtilis, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Salmonella typhi, Staphylococcus aureus, Streptococcus faecalis and Yersinia enterocolitica. It has been showed that the methanol extracts had wider range of activity on these organisms than the aqueous extracts, which indicates that the methanol extracts of all selected plants may contain the active components. This study supports, the traditional medicines (herbal extracts) to cure many diseases like diarrhea, intestinal tract, throat, ear infections, fever and skin diseases. Key words: Antioxidant Medicinal plants Human pathogens
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几种植物的体外抗氧化能力比较
在传统医学中,利用植物治疗烧伤、皮肤病和传染病是很常见的。基于民族药理学和分类学资料,采用琼脂扩散法测定了部分药用植物水提液和醇提液对人致病菌的体外抑菌活性。对五种不同科的具有民族药用价值的植物的叶片进行了抑菌活性研究。所有选择的植物的粉末状叶物质用水和甲醇提取。溶剂萃取物用旋转闪蒸器蒸发至干燥。将干燥的残渣溶于乙醇(1:10 w/v)中,测试其抗菌活性。对蜡样芽孢杆菌、巨型芽孢杆菌、枯草芽孢杆菌、大肠杆菌、肺炎克雷伯菌、铜绿假单胞菌、伤寒沙门氏菌、金黄色葡萄球菌、粪链球菌和小肠结肠炎耶尔森菌进行了体外抗菌筛选。研究表明,甲醇提取物对这些生物的活性范围比水提取物大,这表明所有选定的植物的甲醇提取物都可能含有活性成分。这项研究支持,传统药物(草药提取物)治疗许多疾病,如腹泻,肠道,咽喉,耳部感染,发烧和皮肤病。关键词:抗氧化剂;药用植物;人体病原体
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