Endophytic fungi isolated from the root bark of sungkai (Peronema canescens) as Anti-bacterial and antioxidant

Abstract

Traditional medicine is still used by the community to prevent and treat various diseases, including infectious diseases and exposure the free radicals. The part of the medicinal plant that is often used as traditional medicine is root bark of sungkai. This study explored endophytic fungi from root bark of sungkai and determined their antibacterial and antioxidant activities. Endophytic fungi were isolated from fresh root bark tissue in PDA (Potato Dextrose Agar) media and identified morphologically. The DPPH (2,2-diphenyl-1-picrylhydrazyl) and diffused-disc paper methods were used for antioxidant and antibacterial. The endophytic fungi extracts showing the best bioactivity were identified molecularly. A total of 6 isolates of endophytic fungi were found in this study (RNA1-RNA6). Antibacterial and antioxidant activity test showed the best activity for RNA4 isolates. Molecular identification results describe it as Penicillium janczewskii. This research can be used as a basic reference for isolating pure compounds that have antibacterial and antioxidant properties through chromatography techniques