THE ROLE OF RLM AND AVRLM GENES IN THE REALIZATION OF RAPE SPECIFIC RESISTANCE TO BLACKLEG

V. Lemesh, G. Mozgova, Yadviga E. Pilyuk, N. E. Horuzhiy, E. S. Byk, A. Burakova
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Abstract

As part of the study, DNA markers for local races L. maculans were identified and found that the AvrLm4-7 sequence in the selected fungus population is specifically recognized by the resistance Rlm4 and Rlm7 genes. The SCAR marker BN204 was identified, which allows us to identify homozygous and heterozygous plants carrying the Rlm4 gene. The work collection, including 22 varieties and 39 hybrids of rape, was analyzed using the DNA marker BN204. PCR results allowed us to establish that the number of individual plants with the resistance Rlm4 gene was higher than the number of stable forms characterized by the infection of leaf explants with pathogen races carrying the AvrLm4-7 sequence. It is supposed that these individual plants contain the Rlm4 gene, but they lack the Rlm7 gene.
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RLM和avrlm基因在油菜黑腿特异性抗性实现中的作用
作为研究的一部分,我们对当地菌种L. maculans的DNA标记进行了鉴定,发现所选真菌群体中的avrl4 -7序列被抗性基因Rlm4和Rlm7特异性识别。鉴定出SCAR标记BN204,使我们能够鉴定携带Rlm4基因的纯合子和杂合子植物。利用DNA标记BN204对油菜22个品种和39个杂交种进行分析。PCR结果表明,具有抗性Rlm4基因的单株数量高于携带avrl4 -7序列的病原菌小种侵染叶片外植体的稳定株数量。据推测,这些单株植物含有Rlm4基因,但缺乏Rlm7基因。
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