{"title":"Total Internal Reflection Fluorescence (TIRF) Microscopy","authors":"S. Denham, Deborah Cutchey","doi":"10.1002/IMIC.200990043","DOIUrl":null,"url":null,"abstract":"TIRF microscopy is a technique for imaging the surface of cells that utilizes a thin field of illumination to image fluorophores on or near to the coverslip. TIRF is a highly sensitive widefield camera-based technique providing an excellent signal-to-noise ratio compared with epi-fluorescence or confocal imaging methods. TIRF can be used alone or in combination with other imaging techniques to give an “inside and out” view of a specimen. This technical note describes the theoretical basis of TIRF microscopy, the hardware required and examples of TIRF applications.","PeriodicalId":100658,"journal":{"name":"Imaging & Microscopy","volume":"201 1","pages":"54-55"},"PeriodicalIF":0.0000,"publicationDate":"2009-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Imaging & Microscopy","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1002/IMIC.200990043","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1
Abstract
TIRF microscopy is a technique for imaging the surface of cells that utilizes a thin field of illumination to image fluorophores on or near to the coverslip. TIRF is a highly sensitive widefield camera-based technique providing an excellent signal-to-noise ratio compared with epi-fluorescence or confocal imaging methods. TIRF can be used alone or in combination with other imaging techniques to give an “inside and out” view of a specimen. This technical note describes the theoretical basis of TIRF microscopy, the hardware required and examples of TIRF applications.
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