F. Pérez-Cota, Richard J. Smith, E. Moradi, Anaid Lugo Leija, K. Velickovic, L. Marques, K. Webb, M. Symonds, V. Sottile, Hanny Elsheikha, M. Clark
The mechanical properties of cells play an important role in cell function and behavior. This paper presents recent developments that have enabled the use of laser-generated phonons (ultrasound) with sub-optical wavelengths to look inside living cells. The phonons reveal contrast from changes in the elasticity of the cell and can provide high resolution three dimensional images.
{"title":"Cell imaging by phonon microscopy: sub-optical wavelength ultrasound for non-invasive imaging","authors":"F. Pérez-Cota, Richard J. Smith, E. Moradi, Anaid Lugo Leija, K. Velickovic, L. Marques, K. Webb, M. Symonds, V. Sottile, Hanny Elsheikha, M. Clark","doi":"10.1002/IMAGING.6031","DOIUrl":"https://doi.org/10.1002/IMAGING.6031","url":null,"abstract":"The mechanical properties of cells play an important role in cell function and behavior. This paper presents recent developments that have enabled the use of laser-generated phonons (ultrasound) with sub-optical wavelengths to look inside living cells. The phonons reveal contrast from changes in the elasticity of the cell and can provide high resolution three dimensional images.","PeriodicalId":100658,"journal":{"name":"Imaging & Microscopy","volume":"287 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75635202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
After the successful FOM2009 conference held in Krakow, Poland this year, the next conference Focus on Microscopy 2010 takes place in Shanghai, China. It will be in the week before Easter from Sunday March 28 to Wednesday March 31, 2010. Hosted by the Shanghai Jiao Tong University, it starts around 6 pm with a plenary opening session followed by a welcome reception.
{"title":"Focus on Microscopy 2010","authors":"Q. Ren, Fred Brakenhoff","doi":"10.1002/IMIC.200990074","DOIUrl":"https://doi.org/10.1002/IMIC.200990074","url":null,"abstract":"After the successful FOM2009 conference held in Krakow, Poland this year, the next conference Focus on Microscopy 2010 takes place in Shanghai, China. It will be in the week before Easter from Sunday March 28 to Wednesday March 31, 2010. Hosted by the Shanghai Jiao Tong University, it starts around 6 pm with a plenary opening session followed by a welcome reception.","PeriodicalId":100658,"journal":{"name":"Imaging & Microscopy","volume":"8 1","pages":"14-14"},"PeriodicalIF":0.0,"publicationDate":"2009-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77164185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
With the growing interest of consumers in a healthy and active lifestyle, high quality products are needed that will provide guilt-free eating experiences. As part of Unilever's Vitality mission, the company is committed to providing healthier products for its consumers. Because of this, they are actively looking for value added ingredients that will meet consumer acceptability.
{"title":"Imaging of Plant Material for Food Emulsion Structuring","authors":"M. V. Ruijven, G. Dalen, J. Nijsse, S. Regismond","doi":"10.1002/IMIC.200990083","DOIUrl":"https://doi.org/10.1002/IMIC.200990083","url":null,"abstract":"With the growing interest of consumers in a healthy and active lifestyle, high quality products are needed that will provide guilt-free eating experiences. As part of Unilever's Vitality mission, the company is committed to providing healthier products for its consumers. Because of this, they are actively looking for value added ingredients that will meet consumer acceptability.","PeriodicalId":100658,"journal":{"name":"Imaging & Microscopy","volume":"33 1","pages":"32-34"},"PeriodicalIF":0.0,"publicationDate":"2009-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88988891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Total Internal Reflection Fluorescence Microscopy (TIRFM) is a powerful technique for imaging dynamic membrane events in living cells. However the information gained from TIRFM may be limited by the intensity of the florescence signal and by background noise emanating from the inner part of the cell. Here we describe how Surface Plasmon Mediated Fluorescence Microscopy (SPMFM) can enhance TIRFM by increasing the fluorescence signal 5-6 fold and by reducing background noise dramatically. The imaging configuration is that for TIRFM except that the coverglass is coated on one side with a nanometre film of silver. For SPMFM (as in TIRFM), a high numerical aperture (N.A.) objective is essential for achieving required illumination angles.
{"title":"Surface Plasmon Enhanced TIRF Imaging","authors":"K. Balaa, E. Fort, Nikon Instruments","doi":"10.1002/IMIC.200990091","DOIUrl":"https://doi.org/10.1002/IMIC.200990091","url":null,"abstract":"Total Internal Reflection Fluorescence Microscopy (TIRFM) is a powerful technique for imaging dynamic membrane events in living cells. However the information gained from TIRFM may be limited by the intensity of the florescence signal and by background noise emanating from the inner part of the cell. Here we describe how Surface Plasmon Mediated Fluorescence Microscopy (SPMFM) can enhance TIRFM by increasing the fluorescence signal 5-6 fold and by reducing background noise dramatically. The imaging configuration is that for TIRFM except that the coverglass is coated on one side with a nanometre film of silver. For SPMFM (as in TIRFM), a high numerical aperture (N.A.) objective is essential for achieving required illumination angles.","PeriodicalId":100658,"journal":{"name":"Imaging & Microscopy","volume":"58 1","pages":"55-56"},"PeriodicalIF":0.0,"publicationDate":"2009-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79316047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Although the previous EMS newsletter was published not so very long ago, many important matters have evolved since then. First of all, the MC2009 congress, joining the 9th Multinational Congress on Microscopy and the Dreilandertagung, was organized in Graz, Austria, from August 30 till September 4, 2009. The conference was a great success, not only by counting the large number of attendants, in particular young researchers, but also judging from the many outstanding lectures and posters presenting the newest results in the field of microscopy. Also, plenary and invited lectures dedicated to the 100d birthday of Otto Scherzer and referring to the 50th anniversary of the famous Feynman lecture “There's plenty of room at the bottom” were very much appreciated. The success and the increasing importance of these large regional meetings in different parts of Europe are becoming more and more clear. At Graz, already several hosts for similar future joint meetings were decided, which we believe is a very promising trend.
{"title":"EMS Newsletter 28, October 2009","authors":"P. Midgley, N. Schryvers","doi":"10.1002/IMIC.200990069","DOIUrl":"https://doi.org/10.1002/IMIC.200990069","url":null,"abstract":"Although the previous EMS newsletter was published not so very long ago, many important matters have evolved since then. First of all, the MC2009 congress, joining the 9th Multinational Congress on Microscopy and the Dreilandertagung, was organized in Graz, Austria, from August 30 till September 4, 2009. The conference was a great success, not only by counting the large number of attendants, in particular young researchers, but also judging from the many outstanding lectures and posters presenting the newest results in the field of microscopy. Also, plenary and invited lectures dedicated to the 100d birthday of Otto Scherzer and referring to the 50th anniversary of the famous Feynman lecture “There's plenty of room at the bottom” were very much appreciated. The success and the increasing importance of these large regional meetings in different parts of Europe are becoming more and more clear. At Graz, already several hosts for similar future joint meetings were decided, which we believe is a very promising trend.","PeriodicalId":100658,"journal":{"name":"Imaging & Microscopy","volume":"12 1","pages":"10-10"},"PeriodicalIF":0.0,"publicationDate":"2009-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88883713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
With the potential to develop into any cell type in the body, stem cells promise new therapeutic possibilities for the treatment of tissues damaged by age-related degeneration, injury or disease.
{"title":"New Developments in Stem Cell Research","authors":"M. Herbert, K. Nayernia, C. Keogh","doi":"10.1002/IMIC.200990082","DOIUrl":"https://doi.org/10.1002/IMIC.200990082","url":null,"abstract":"With the potential to develop into any cell type in the body, stem cells promise new therapeutic possibilities for the treatment of tissues damaged by age-related degeneration, injury or disease.","PeriodicalId":100658,"journal":{"name":"Imaging & Microscopy","volume":"125 1","pages":"29-31"},"PeriodicalIF":0.0,"publicationDate":"2009-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84953290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The human eye is a powerful and sensitive instrument which excels in pattern recognition, by using mental templates to identify and categorize objects. Unfortunately, its exact strength may also be its weakness, for it implies a certain subjectivity to the interpretation of images. Furthermore, our distinctive power and vocabulary for describing objects in a quantitative manner are limited. Accurate visualization and characterization of cellular features thus calls for a more objective approach, which can now be provided by the fast growing discipline of high content image cytometry (HCIC).
{"title":"High Content Image Cytometry: A Versatile Tool for Mapping Subnuclear Organization","authors":"W. D. Vos, P. Oostveldt, E. Manders","doi":"10.1002/IMIC.200990089","DOIUrl":"https://doi.org/10.1002/IMIC.200990089","url":null,"abstract":"The human eye is a powerful and sensitive instrument which excels in pattern recognition, by using mental templates to identify and categorize objects. Unfortunately, its exact strength may also be its weakness, for it implies a certain subjectivity to the interpretation of images. Furthermore, our distinctive power and vocabulary for describing objects in a quantitative manner are limited. Accurate visualization and characterization of cellular features thus calls for a more objective approach, which can now be provided by the fast growing discipline of high content image cytometry (HCIC).","PeriodicalId":100658,"journal":{"name":"Imaging & Microscopy","volume":"115 1","pages":"46-48"},"PeriodicalIF":0.0,"publicationDate":"2009-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80137127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Microscopy and Microanalysis 2009","authors":"David J. Smith","doi":"10.1002/IMIC.200990078","DOIUrl":"https://doi.org/10.1002/IMIC.200990078","url":null,"abstract":"","PeriodicalId":100658,"journal":{"name":"Imaging & Microscopy","volume":"64 1","pages":"20-20"},"PeriodicalIF":0.0,"publicationDate":"2009-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91145277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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