Inhibitory effect of Dalbergia latifoia extract on acetylcholinesterase enzyme by invitro methods

YMER Digital Pub Date : 2022-08-17 DOI:10.37896/ymer21.08/60

D. P

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Abstract

The present study was conducted to evaluate acetylcholinesterase (AChE) inhibitory effects of Dalbergia latifoia. The hexane, ethylacetate and ethanolic extract of Dalbergia latifoia were tested for their invitro anticholinesterase inhibitory effect by spectrophotometeric and by TLC bioassay method.The result revealed that ethanolic extract of Dalbergia latifoia showed better anticholinesterase inhibition compared to other extracts. The most active one was found to be ethanolic extract of Dalbergia latifoia having IC50 value at 81±1.56 µg/ml. Hexane extract of Dalbergia latifoia has not shown any anticholinesterase inhibitory effect. Ethylacetate extract of Dalbergia latifoia was found to have IC50 value at 625±2.65 µg/ml. TLC bioassay is an easier and rapid means for detection of enzyme inhibition. The hexane, ethylacetate and ethanolic extract of Dalbergia latifoia were tested for their anticholinesterase inhibition by TLC bioassay TLC bioassay is an easier and rapid means for detection of enzyme inhibition. The active spots appearedas white spots on yellow background. Ethanolic extract of Dalbergia latifoia showed more than one active spots compared to other extracts. The TLC assay also demonstrated AchE inhibitory activity for ethanolic extract of Dalbergia latifoia. Keywords: Dalbergia latifoia, acetylcholinesterase, Alzheimer’s disease, TLC bioautography

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黄檀提取物对乙酰胆碱酯酶的体外抑制作用

本文研究了黄檀对乙酰胆碱酯酶(AChE)的抑制作用。采用分光光度法和薄层色谱法测定了黄檀正己烷、乙酸乙酯和乙醇提取物的体外抗胆碱酯酶抑制作用。结果表明，黄檀乙醇提取物具有较好的抗胆碱酯酶抑制作用。其中活性最强的是黄檀醇提物，IC50值为81±1.56µg/ml。黄檀己烷提取物对抗胆碱酯酶无明显抑制作用。黄檀乙酸乙酯提取物的IC50值为625±2.65µg/ml。薄层色谱法是一种简便、快速的酶抑制检测方法。采用薄层色谱法测定了黄檀正己烷、乙酸乙酯和乙醇提取物的抗胆碱酯酶抑制作用，是一种简便、快速的酶抑制检测方法。活动的斑点在黄色的背景上表现为白色的斑点。黄檀醇提物与其他提取物相比，有一个以上的活性点。薄层色谱法也证实了黄檀醇提物对乙酰胆碱酯酶的抑制作用。关键词:黄檀;乙酰胆碱酯酶;阿尔茨海默病

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