Analysis of bioactive chemical compounds of Euphorbia lathyrus using gas chromatography-mass spectrometry and Fourier-transform infrared spectroscopy

Abstract

The aim of this study was determination the phytochemical composition of methanolic seeds extract of Euphorbia lathyrus. Gas chromatography-mass spectrometry (GC-MS) analysis of E. lathyrus revealed the existence of the Carbonic acid, (ethyl)(1,2,4-triazol-1-ylmethyl) diester, 1H-Pyrrole,2,5-dihydro-1-nitroso, Hexanal dimethyl acetal,  Isosorbide dinitrate, DL-Arabinose, Cyclopropane,1-fluoro-1-(2-bromoethenyl)-2,2,3,3-tetramethyl, α-D-Glucopyranoside, O-α-D-glucopyranosyl-(1.fwdarw.3)-s-d-fruc, Desulphosinigrin, D-Glucose, 6-O-α-D-galactopyranosyl, Octanoic acid, Benzofuran,2,3-dihydro, 6-Acetyl-s-d-mannose, Estragole, Ascaridole epoxide, 3-Allyl-6-methoxyphenol, 4-Amino-1,5,pentandioic acid, l-Gala-l-ido-octonic lactone, y-Sitosterol, Tetradecanoic acid, l-(+)-Ascorbic acid 2,6-dihexadecanoate, Estra -1,3,5(10)-trien-17s-ol, Propanoic acid,2-(3-acetoxy-4,4,14-trimethylandrost-8-en-17-yl), Cis-13-Eicosenoic acid, Eicosanoic acid, 3-Pyrinecarboxylic acid, 2,7,10-tris(acetyloxy)-1,1a,2,3,4,6,7,10, Oleic acid, eicosyl ester, Butanoic acid, 4-chloro-,1,1a,1b,4,4a,5,7a,7b,8,9-decahydro-4a, Ethyl iso–allocholate, Ethyl iso –allocholate, Olean-12-ene-3,15,16,21,22,28-hexol, (3s,15α,16α,21s,22α)- and 2,4,6-Decatrienoic  acid,1a,2,5,5a,6,9,10,10a-octahydro-5,5a-dihy. The Fourier-transform infrared spectroscopy (FTIR) analysis of E. lathyrus seeds proved the presence of alkenes, aliphatic fluoro compounds, alcohols, ethers, carboxlic acids, esters, nitro compounds, alkanes, hydrogen bonded alcohols, and phenols.   Key words: Gas chromatography-mass spectrometry (GC/MS), bioactive compounds, Fourier-transform infrared spectroscopy (FT-IR), Euphorbia lathyrus.