Neurospheroid array on a flexible substrate for cortical microstimulation

Abstract

This paper describes a centimeter-sized flexible multi-electrode array (MEA) integrated with a neuronal spheroid (neurospheroid) array. The MEA has a number of neurospheroids and can be applied to extensively curved surfaces (e.g., brain). We demonstrated that MEA successfully activated neurons within a neurospheroid on the microelectrode array. In addition, the MEA was used to activate 2D culture of cortical cells through the neurospheroids. These results mark an important step toward achieving minimally-invasive cortical microstimulation via neurospheroid-based stimulating systems.